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The presence of SARS-CoV-2 in raw and treated wastewater in 3 cities of Iran: Tehran, Qom and Anzali during coronavirus disease 2019 (COVID-19) outbreak
This study aimed to identifying the presence of SARS-CoV-2 RNA in raw and treated wastewater during the COVID-19 outbreak in Tehran, Qom and Anzali cities (Iran). From three wastewater treatment plants (WWTPs), 28 treated and untreated wastewater composite samples were collected from April 4 to May...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903026/ https://www.ncbi.nlm.nih.gov/pubmed/33643658 http://dx.doi.org/10.1007/s40201-021-00629-6 |
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author | Nasseri, Simin Yavarian, Jila Baghani, Abbas Norouzian Azad, Talat Mokhtari Nejati, Ahmad Nabizadeh, Ramin Hadi, Mahdi Jandaghi, Nazanin Zahra Shafiei Vakili, Behnam Vaghefi, Seyed Koushyar Azam Baghban, Mahtab Yousefi, Somayeh Nazmara, Shahrokh Alimohammadi, Mahmood |
author_facet | Nasseri, Simin Yavarian, Jila Baghani, Abbas Norouzian Azad, Talat Mokhtari Nejati, Ahmad Nabizadeh, Ramin Hadi, Mahdi Jandaghi, Nazanin Zahra Shafiei Vakili, Behnam Vaghefi, Seyed Koushyar Azam Baghban, Mahtab Yousefi, Somayeh Nazmara, Shahrokh Alimohammadi, Mahmood |
author_sort | Nasseri, Simin |
collection | PubMed |
description | This study aimed to identifying the presence of SARS-CoV-2 RNA in raw and treated wastewater during the COVID-19 outbreak in Tehran, Qom and Anzali cities (Iran). From three wastewater treatment plants (WWTPs), 28 treated and untreated wastewater composite samples were collected from April 4 to May 2, 2020. In this study, polyethylene glycol 6000 (PEG 6000) was used through one-step real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) for identification of RNA viruses. SARS-CoV-2 RNA was elicited from wastewater composite samples in all inlet samples taken from the three above mentioned cities. The results of outlet samples were as follows: 1) Results from Qom and East Anzali outlets showed no trace of SARS-CoV-2 RNA despite the difference in treatment disinfection method used (chlorine vs. ultraviolet (UV) disinfection). 2. In Tehran, SARS-CoV-2 RNA was not detected in any of the outlet samples taken from the modules disinfected by UV. Out of the four samples taken from the modules disinfected by chlorine, two were positive for the SARS-CoV-2 RNA which could have been caused by deficiencies in operation and maintenance. It can be concluded that meeting the standards of operation and maintenance (O&M) in WWTPs can considerably ensure that wastewater does not act as one of the roots of transmission for the disease. |
format | Online Article Text |
id | pubmed-7903026 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-79030262021-02-24 The presence of SARS-CoV-2 in raw and treated wastewater in 3 cities of Iran: Tehran, Qom and Anzali during coronavirus disease 2019 (COVID-19) outbreak Nasseri, Simin Yavarian, Jila Baghani, Abbas Norouzian Azad, Talat Mokhtari Nejati, Ahmad Nabizadeh, Ramin Hadi, Mahdi Jandaghi, Nazanin Zahra Shafiei Vakili, Behnam Vaghefi, Seyed Koushyar Azam Baghban, Mahtab Yousefi, Somayeh Nazmara, Shahrokh Alimohammadi, Mahmood J Environ Health Sci Eng Research Article This study aimed to identifying the presence of SARS-CoV-2 RNA in raw and treated wastewater during the COVID-19 outbreak in Tehran, Qom and Anzali cities (Iran). From three wastewater treatment plants (WWTPs), 28 treated and untreated wastewater composite samples were collected from April 4 to May 2, 2020. In this study, polyethylene glycol 6000 (PEG 6000) was used through one-step real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) for identification of RNA viruses. SARS-CoV-2 RNA was elicited from wastewater composite samples in all inlet samples taken from the three above mentioned cities. The results of outlet samples were as follows: 1) Results from Qom and East Anzali outlets showed no trace of SARS-CoV-2 RNA despite the difference in treatment disinfection method used (chlorine vs. ultraviolet (UV) disinfection). 2. In Tehran, SARS-CoV-2 RNA was not detected in any of the outlet samples taken from the modules disinfected by UV. Out of the four samples taken from the modules disinfected by chlorine, two were positive for the SARS-CoV-2 RNA which could have been caused by deficiencies in operation and maintenance. It can be concluded that meeting the standards of operation and maintenance (O&M) in WWTPs can considerably ensure that wastewater does not act as one of the roots of transmission for the disease. Springer International Publishing 2021-02-24 /pmc/articles/PMC7903026/ /pubmed/33643658 http://dx.doi.org/10.1007/s40201-021-00629-6 Text en © Springer Nature Switzerland AG 2021 |
spellingShingle | Research Article Nasseri, Simin Yavarian, Jila Baghani, Abbas Norouzian Azad, Talat Mokhtari Nejati, Ahmad Nabizadeh, Ramin Hadi, Mahdi Jandaghi, Nazanin Zahra Shafiei Vakili, Behnam Vaghefi, Seyed Koushyar Azam Baghban, Mahtab Yousefi, Somayeh Nazmara, Shahrokh Alimohammadi, Mahmood The presence of SARS-CoV-2 in raw and treated wastewater in 3 cities of Iran: Tehran, Qom and Anzali during coronavirus disease 2019 (COVID-19) outbreak |
title | The presence of SARS-CoV-2 in raw and treated wastewater in 3 cities of Iran: Tehran, Qom and Anzali during coronavirus disease 2019 (COVID-19) outbreak |
title_full | The presence of SARS-CoV-2 in raw and treated wastewater in 3 cities of Iran: Tehran, Qom and Anzali during coronavirus disease 2019 (COVID-19) outbreak |
title_fullStr | The presence of SARS-CoV-2 in raw and treated wastewater in 3 cities of Iran: Tehran, Qom and Anzali during coronavirus disease 2019 (COVID-19) outbreak |
title_full_unstemmed | The presence of SARS-CoV-2 in raw and treated wastewater in 3 cities of Iran: Tehran, Qom and Anzali during coronavirus disease 2019 (COVID-19) outbreak |
title_short | The presence of SARS-CoV-2 in raw and treated wastewater in 3 cities of Iran: Tehran, Qom and Anzali during coronavirus disease 2019 (COVID-19) outbreak |
title_sort | presence of sars-cov-2 in raw and treated wastewater in 3 cities of iran: tehran, qom and anzali during coronavirus disease 2019 (covid-19) outbreak |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903026/ https://www.ncbi.nlm.nih.gov/pubmed/33643658 http://dx.doi.org/10.1007/s40201-021-00629-6 |
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