Cargando…

The presence of SARS-CoV-2 in raw and treated wastewater in 3 cities of Iran: Tehran, Qom and Anzali during coronavirus disease 2019 (COVID-19) outbreak

This study aimed to identifying the presence of SARS-CoV-2 RNA in raw and treated wastewater during the COVID-19 outbreak in Tehran, Qom and Anzali cities (Iran). From three wastewater treatment plants (WWTPs), 28 treated and untreated wastewater composite samples were collected from April 4 to May...

Descripción completa

Detalles Bibliográficos
Autores principales: Nasseri, Simin, Yavarian, Jila, Baghani, Abbas Norouzian, Azad, Talat Mokhtari, Nejati, Ahmad, Nabizadeh, Ramin, Hadi, Mahdi, Jandaghi, Nazanin Zahra Shafiei, Vakili, Behnam, Vaghefi, Seyed Koushyar Azam, Baghban, Mahtab, Yousefi, Somayeh, Nazmara, Shahrokh, Alimohammadi, Mahmood
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903026/
https://www.ncbi.nlm.nih.gov/pubmed/33643658
http://dx.doi.org/10.1007/s40201-021-00629-6
_version_ 1783654656519438336
author Nasseri, Simin
Yavarian, Jila
Baghani, Abbas Norouzian
Azad, Talat Mokhtari
Nejati, Ahmad
Nabizadeh, Ramin
Hadi, Mahdi
Jandaghi, Nazanin Zahra Shafiei
Vakili, Behnam
Vaghefi, Seyed Koushyar Azam
Baghban, Mahtab
Yousefi, Somayeh
Nazmara, Shahrokh
Alimohammadi, Mahmood
author_facet Nasseri, Simin
Yavarian, Jila
Baghani, Abbas Norouzian
Azad, Talat Mokhtari
Nejati, Ahmad
Nabizadeh, Ramin
Hadi, Mahdi
Jandaghi, Nazanin Zahra Shafiei
Vakili, Behnam
Vaghefi, Seyed Koushyar Azam
Baghban, Mahtab
Yousefi, Somayeh
Nazmara, Shahrokh
Alimohammadi, Mahmood
author_sort Nasseri, Simin
collection PubMed
description This study aimed to identifying the presence of SARS-CoV-2 RNA in raw and treated wastewater during the COVID-19 outbreak in Tehran, Qom and Anzali cities (Iran). From three wastewater treatment plants (WWTPs), 28 treated and untreated wastewater composite samples were collected from April 4 to May 2, 2020. In this study, polyethylene glycol 6000 (PEG 6000) was used through one-step real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) for identification of RNA viruses. SARS-CoV-2 RNA was elicited from wastewater composite samples in all inlet samples taken from the three above mentioned cities. The results of outlet samples were as follows: 1) Results from Qom and East Anzali outlets showed no trace of SARS-CoV-2 RNA despite the difference in treatment disinfection method used (chlorine vs. ultraviolet (UV) disinfection). 2. In Tehran, SARS-CoV-2 RNA was not detected in any of the outlet samples taken from the modules disinfected by UV. Out of the four samples taken from the modules disinfected by chlorine, two were positive for the SARS-CoV-2 RNA which could have been caused by deficiencies in operation and maintenance. It can be concluded that meeting the standards of operation and maintenance (O&M) in WWTPs can considerably ensure that wastewater does not act as one of the roots of transmission for the disease.
format Online
Article
Text
id pubmed-7903026
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Springer International Publishing
record_format MEDLINE/PubMed
spelling pubmed-79030262021-02-24 The presence of SARS-CoV-2 in raw and treated wastewater in 3 cities of Iran: Tehran, Qom and Anzali during coronavirus disease 2019 (COVID-19) outbreak Nasseri, Simin Yavarian, Jila Baghani, Abbas Norouzian Azad, Talat Mokhtari Nejati, Ahmad Nabizadeh, Ramin Hadi, Mahdi Jandaghi, Nazanin Zahra Shafiei Vakili, Behnam Vaghefi, Seyed Koushyar Azam Baghban, Mahtab Yousefi, Somayeh Nazmara, Shahrokh Alimohammadi, Mahmood J Environ Health Sci Eng Research Article This study aimed to identifying the presence of SARS-CoV-2 RNA in raw and treated wastewater during the COVID-19 outbreak in Tehran, Qom and Anzali cities (Iran). From three wastewater treatment plants (WWTPs), 28 treated and untreated wastewater composite samples were collected from April 4 to May 2, 2020. In this study, polyethylene glycol 6000 (PEG 6000) was used through one-step real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) for identification of RNA viruses. SARS-CoV-2 RNA was elicited from wastewater composite samples in all inlet samples taken from the three above mentioned cities. The results of outlet samples were as follows: 1) Results from Qom and East Anzali outlets showed no trace of SARS-CoV-2 RNA despite the difference in treatment disinfection method used (chlorine vs. ultraviolet (UV) disinfection). 2. In Tehran, SARS-CoV-2 RNA was not detected in any of the outlet samples taken from the modules disinfected by UV. Out of the four samples taken from the modules disinfected by chlorine, two were positive for the SARS-CoV-2 RNA which could have been caused by deficiencies in operation and maintenance. It can be concluded that meeting the standards of operation and maintenance (O&M) in WWTPs can considerably ensure that wastewater does not act as one of the roots of transmission for the disease. Springer International Publishing 2021-02-24 /pmc/articles/PMC7903026/ /pubmed/33643658 http://dx.doi.org/10.1007/s40201-021-00629-6 Text en © Springer Nature Switzerland AG 2021
spellingShingle Research Article
Nasseri, Simin
Yavarian, Jila
Baghani, Abbas Norouzian
Azad, Talat Mokhtari
Nejati, Ahmad
Nabizadeh, Ramin
Hadi, Mahdi
Jandaghi, Nazanin Zahra Shafiei
Vakili, Behnam
Vaghefi, Seyed Koushyar Azam
Baghban, Mahtab
Yousefi, Somayeh
Nazmara, Shahrokh
Alimohammadi, Mahmood
The presence of SARS-CoV-2 in raw and treated wastewater in 3 cities of Iran: Tehran, Qom and Anzali during coronavirus disease 2019 (COVID-19) outbreak
title The presence of SARS-CoV-2 in raw and treated wastewater in 3 cities of Iran: Tehran, Qom and Anzali during coronavirus disease 2019 (COVID-19) outbreak
title_full The presence of SARS-CoV-2 in raw and treated wastewater in 3 cities of Iran: Tehran, Qom and Anzali during coronavirus disease 2019 (COVID-19) outbreak
title_fullStr The presence of SARS-CoV-2 in raw and treated wastewater in 3 cities of Iran: Tehran, Qom and Anzali during coronavirus disease 2019 (COVID-19) outbreak
title_full_unstemmed The presence of SARS-CoV-2 in raw and treated wastewater in 3 cities of Iran: Tehran, Qom and Anzali during coronavirus disease 2019 (COVID-19) outbreak
title_short The presence of SARS-CoV-2 in raw and treated wastewater in 3 cities of Iran: Tehran, Qom and Anzali during coronavirus disease 2019 (COVID-19) outbreak
title_sort presence of sars-cov-2 in raw and treated wastewater in 3 cities of iran: tehran, qom and anzali during coronavirus disease 2019 (covid-19) outbreak
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903026/
https://www.ncbi.nlm.nih.gov/pubmed/33643658
http://dx.doi.org/10.1007/s40201-021-00629-6
work_keys_str_mv AT nasserisimin thepresenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT yavarianjila thepresenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT baghaniabbasnorouzian thepresenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT azadtalatmokhtari thepresenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT nejatiahmad thepresenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT nabizadehramin thepresenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT hadimahdi thepresenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT jandaghinazaninzahrashafiei thepresenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT vakilibehnam thepresenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT vaghefiseyedkoushyarazam thepresenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT baghbanmahtab thepresenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT yousefisomayeh thepresenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT nazmarashahrokh thepresenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT alimohammadimahmood thepresenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT nasserisimin presenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT yavarianjila presenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT baghaniabbasnorouzian presenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT azadtalatmokhtari presenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT nejatiahmad presenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT nabizadehramin presenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT hadimahdi presenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT jandaghinazaninzahrashafiei presenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT vakilibehnam presenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT vaghefiseyedkoushyarazam presenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT baghbanmahtab presenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT yousefisomayeh presenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT nazmarashahrokh presenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak
AT alimohammadimahmood presenceofsarscov2inrawandtreatedwastewaterin3citiesofirantehranqomandanzaliduringcoronavirusdisease2019covid19outbreak