β-sitosterol Mediated Silver Nanoparticles Induce Cytotoxicity in Human Colon Cancer HT-29 Cells

BACKGROUND: Silver nanoparticles (AgNP) are commonly used metallic nanoparticles in health care systems. Colon cancer incidence is increasing worldwide. In this study, AgNP was synthesized using β-sitosterol and its cytotoxic potential was evaluated in human colon cancer (HT-29) cells. METHODS: Char...

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Autores principales: Shathviha, Palaniappan Chithambara, Ezhilarasan, Devaraj, Rajeshkumar, Shanmugam, Selvaraj, Jayaraman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Avicenna Research Institute 2021
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903430/
https://www.ncbi.nlm.nih.gov/pubmed/33680372
http://dx.doi.org/10.18502/ajmb.v13i1.4577
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author Shathviha, Palaniappan Chithambara
Ezhilarasan, Devaraj
Rajeshkumar, Shanmugam
Selvaraj, Jayaraman
author_facet Shathviha, Palaniappan Chithambara
Ezhilarasan, Devaraj
Rajeshkumar, Shanmugam
Selvaraj, Jayaraman
author_sort Shathviha, Palaniappan Chithambara
collection PubMed
description BACKGROUND: Silver nanoparticles (AgNP) are commonly used metallic nanoparticles in health care systems. Colon cancer incidence is increasing worldwide. In this study, AgNP was synthesized using β-sitosterol and its cytotoxic potential was evaluated in human colon cancer (HT-29) cells. METHODS: Characterization of AgNP was analyzed by TEM and spectrophotometry analysis. HT-29 cells were treated with different concentrations (2, 4, 6, 8 and 10 ng/ml) of AgNPs and cytotoxicity was evaluated by MTT assay. The apoptosis was analyzed by the flow cytometry. The expression of p53 protein was analyzed by western blotting. RESULTS: β-sitosterol mediated AgNP are spherical in shape and induced concentration-dependent cytotoxicity in HT-29 cells. AgNP caused apoptosis related morphological changes as evidenced by annexin positive staining. AgNP treatments also induced the p53 expression in HT-29 cells. CONCLUSION: Our present result suggests that β-sitosterol mediated AgNP induce apoptosis in colon cancer cells and this finding may pave the way for further experimental analysis in vivo.
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spelling pubmed-79034302021-03-04 β-sitosterol Mediated Silver Nanoparticles Induce Cytotoxicity in Human Colon Cancer HT-29 Cells Shathviha, Palaniappan Chithambara Ezhilarasan, Devaraj Rajeshkumar, Shanmugam Selvaraj, Jayaraman Avicenna J Med Biotechnol Short Communication BACKGROUND: Silver nanoparticles (AgNP) are commonly used metallic nanoparticles in health care systems. Colon cancer incidence is increasing worldwide. In this study, AgNP was synthesized using β-sitosterol and its cytotoxic potential was evaluated in human colon cancer (HT-29) cells. METHODS: Characterization of AgNP was analyzed by TEM and spectrophotometry analysis. HT-29 cells were treated with different concentrations (2, 4, 6, 8 and 10 ng/ml) of AgNPs and cytotoxicity was evaluated by MTT assay. The apoptosis was analyzed by the flow cytometry. The expression of p53 protein was analyzed by western blotting. RESULTS: β-sitosterol mediated AgNP are spherical in shape and induced concentration-dependent cytotoxicity in HT-29 cells. AgNP caused apoptosis related morphological changes as evidenced by annexin positive staining. AgNP treatments also induced the p53 expression in HT-29 cells. CONCLUSION: Our present result suggests that β-sitosterol mediated AgNP induce apoptosis in colon cancer cells and this finding may pave the way for further experimental analysis in vivo. Avicenna Research Institute 2021 /pmc/articles/PMC7903430/ /pubmed/33680372 http://dx.doi.org/10.18502/ajmb.v13i1.4577 Text en Copyright© 2021 Avicenna Research Institute http://creativecommons.org/licenses/by-nc/4.0/ This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
spellingShingle Short Communication
Shathviha, Palaniappan Chithambara
Ezhilarasan, Devaraj
Rajeshkumar, Shanmugam
Selvaraj, Jayaraman
β-sitosterol Mediated Silver Nanoparticles Induce Cytotoxicity in Human Colon Cancer HT-29 Cells
title β-sitosterol Mediated Silver Nanoparticles Induce Cytotoxicity in Human Colon Cancer HT-29 Cells
title_full β-sitosterol Mediated Silver Nanoparticles Induce Cytotoxicity in Human Colon Cancer HT-29 Cells
title_fullStr β-sitosterol Mediated Silver Nanoparticles Induce Cytotoxicity in Human Colon Cancer HT-29 Cells
title_full_unstemmed β-sitosterol Mediated Silver Nanoparticles Induce Cytotoxicity in Human Colon Cancer HT-29 Cells
title_short β-sitosterol Mediated Silver Nanoparticles Induce Cytotoxicity in Human Colon Cancer HT-29 Cells
title_sort β-sitosterol mediated silver nanoparticles induce cytotoxicity in human colon cancer ht-29 cells
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903430/
https://www.ncbi.nlm.nih.gov/pubmed/33680372
http://dx.doi.org/10.18502/ajmb.v13i1.4577
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