Live imaging of microtubule dynamics at excitatory presynaptic boutons in primary hippocampal neurons and acute hippocampal slices

Analyses of microtubule (MT) plus end dynamics at glutamatergic en passant boutons can be carried out in cultured primary neurons isolated from mouse or rat embryos or ex vivo in acute slices isolated from mice that had been electroporated in utero. Here, we describe a protocol for setting up and an...

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Detalles Bibliográficos
Autores principales: Qu, Xiaoyi, Kumar, Atul, Bartolini, Francesca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903459/
https://www.ncbi.nlm.nih.gov/pubmed/33665622
http://dx.doi.org/10.1016/j.xpro.2021.100342
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author Qu, Xiaoyi
Kumar, Atul
Bartolini, Francesca
author_facet Qu, Xiaoyi
Kumar, Atul
Bartolini, Francesca
author_sort Qu, Xiaoyi
collection PubMed
description Analyses of microtubule (MT) plus end dynamics at glutamatergic en passant boutons can be carried out in cultured primary neurons isolated from mouse or rat embryos or ex vivo in acute slices isolated from mice that had been electroporated in utero. Here, we describe a protocol for setting up and analyzing live image recordings of primary neurons and acute hippocampal slices expressing tagged versions of the MT plus end binding protein EB3 and the presynaptic vesicle markers vGlut1 or VAMP2. For complete information on the use and execution of this protocol, please refer to Qu et al. (2019).
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spelling pubmed-79034592021-03-03 Live imaging of microtubule dynamics at excitatory presynaptic boutons in primary hippocampal neurons and acute hippocampal slices Qu, Xiaoyi Kumar, Atul Bartolini, Francesca STAR Protoc Protocol Analyses of microtubule (MT) plus end dynamics at glutamatergic en passant boutons can be carried out in cultured primary neurons isolated from mouse or rat embryos or ex vivo in acute slices isolated from mice that had been electroporated in utero. Here, we describe a protocol for setting up and analyzing live image recordings of primary neurons and acute hippocampal slices expressing tagged versions of the MT plus end binding protein EB3 and the presynaptic vesicle markers vGlut1 or VAMP2. For complete information on the use and execution of this protocol, please refer to Qu et al. (2019). Elsevier 2021-02-21 /pmc/articles/PMC7903459/ /pubmed/33665622 http://dx.doi.org/10.1016/j.xpro.2021.100342 Text en © 2021 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Qu, Xiaoyi
Kumar, Atul
Bartolini, Francesca
Live imaging of microtubule dynamics at excitatory presynaptic boutons in primary hippocampal neurons and acute hippocampal slices
title Live imaging of microtubule dynamics at excitatory presynaptic boutons in primary hippocampal neurons and acute hippocampal slices
title_full Live imaging of microtubule dynamics at excitatory presynaptic boutons in primary hippocampal neurons and acute hippocampal slices
title_fullStr Live imaging of microtubule dynamics at excitatory presynaptic boutons in primary hippocampal neurons and acute hippocampal slices
title_full_unstemmed Live imaging of microtubule dynamics at excitatory presynaptic boutons in primary hippocampal neurons and acute hippocampal slices
title_short Live imaging of microtubule dynamics at excitatory presynaptic boutons in primary hippocampal neurons and acute hippocampal slices
title_sort live imaging of microtubule dynamics at excitatory presynaptic boutons in primary hippocampal neurons and acute hippocampal slices
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903459/
https://www.ncbi.nlm.nih.gov/pubmed/33665622
http://dx.doi.org/10.1016/j.xpro.2021.100342
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AT bartolinifrancesca liveimagingofmicrotubuledynamicsatexcitatorypresynapticboutonsinprimaryhippocampalneuronsandacutehippocampalslices

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