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Purification of DNA repair protein complexes from mammalian cells

Cells possess multiple DNA repair pathways to tackle a variety of DNA lesions. Often, DNA repair proteins function as large protein complexes. Here, we describe a protocol to purify DNA repair protein complexes from nuclei of mammalian cells. The method permits purification of protein complexes cont...

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Detalles Bibliográficos
Autores principales: Liang, Chih-Chao, Cohn, Martin A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903465/
https://www.ncbi.nlm.nih.gov/pubmed/33665628
http://dx.doi.org/10.1016/j.xpro.2021.100348