Cargando…
Effect of intravitreal conbercept injection on VEGF-A and -B levels in the aqueous and vitreous humor of patients with proliferative diabetic retinopathy
The present study aimed to investigate the mechanisms of anti-VEGF treatment prior to eye surgery to reduce intraoperative bleeding. A total of 30 patients with proliferative vitreoretinopathy after clinical diagnosis were enrolled in the present study as the surgical group. Furthermore, 30 patients...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903486/ https://www.ncbi.nlm.nih.gov/pubmed/33732305 http://dx.doi.org/10.3892/etm.2021.9763 |
_version_ | 1783654747828387840 |
---|---|
author | Zhang, Yunda Gao, Zhao Zhang, Ximei Yuan, Zhigang Ma, Tao Li, Gaiyun Gao, Xiaohong |
author_facet | Zhang, Yunda Gao, Zhao Zhang, Ximei Yuan, Zhigang Ma, Tao Li, Gaiyun Gao, Xiaohong |
author_sort | Zhang, Yunda |
collection | PubMed |
description | The present study aimed to investigate the mechanisms of anti-VEGF treatment prior to eye surgery to reduce intraoperative bleeding. A total of 30 patients with proliferative vitreoretinopathy after clinical diagnosis were enrolled in the present study as the surgical group. Furthermore, 30 patients underwent intravitreal injection of the anti-VEGF drug conbercept and were considered the drug pretreatment group. The aqueous and vitreous humors from the eyes of patients in the surgical group were drawn during pars plana vitrectomy surgery. The aqueous humor in the eyes of patients in the drug pretreatment group was drawn prior to conbercept treatment and seven days after conbercept treatment immediately prior to surgery. The vitreous humor in this group was only taken during surgery. Furthermore, ELISA was used to detect the levels of VEGF-A and -B in the aqueous and vitreous humors. Semi-quantitative determination of VEGF-A and VEGF-B levels in fibrovascular proliferative membranes was performed using immunohistochemical staining. The results indicated that in the drug group, the levels of VEGF-A in the aqueous humor of patients prior to and after conbercept injection were 197.66±48.00 and 3.39±2.54 pg/ml, respectively. The levels of VEGF-A in the vitreous humor of patients in the surgical and drug groups were 267.53±179.60 and 21.43±5.81 pg/ml after injection, respectively. The levels of VEGF-B in the aqueous humor of patients prior to and after conbercept injection were 13.66±3.30 (before injection) and 2.17±0.94 pg/ml (after injection), respectively. The levels of VEGF-B in the vitreous humor of patients in the surgical and drug groups were 127.36±16.72 and 18.56±9.82 pg/ml after injection, respectively (P<0.05). Furthermore, in the drug group, the surgery time, bleeding and capillary formation were significantly reduced compared with those in the surgical group. Taken together, these results suggested that the levels of VEGF-A and -B decreased significantly in the aqueous humor of patients with PDR after conbercept injection. Furthermore, the levels of VEGF-A and -B in the vitreous humor of patients dropped significantly in the drug group compared with those in the surgical group. These results provide theoretical clinical support for the preoperative application of conbercept for patients with PDR. |
format | Online Article Text |
id | pubmed-7903486 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-79034862021-03-16 Effect of intravitreal conbercept injection on VEGF-A and -B levels in the aqueous and vitreous humor of patients with proliferative diabetic retinopathy Zhang, Yunda Gao, Zhao Zhang, Ximei Yuan, Zhigang Ma, Tao Li, Gaiyun Gao, Xiaohong Exp Ther Med Articles The present study aimed to investigate the mechanisms of anti-VEGF treatment prior to eye surgery to reduce intraoperative bleeding. A total of 30 patients with proliferative vitreoretinopathy after clinical diagnosis were enrolled in the present study as the surgical group. Furthermore, 30 patients underwent intravitreal injection of the anti-VEGF drug conbercept and were considered the drug pretreatment group. The aqueous and vitreous humors from the eyes of patients in the surgical group were drawn during pars plana vitrectomy surgery. The aqueous humor in the eyes of patients in the drug pretreatment group was drawn prior to conbercept treatment and seven days after conbercept treatment immediately prior to surgery. The vitreous humor in this group was only taken during surgery. Furthermore, ELISA was used to detect the levels of VEGF-A and -B in the aqueous and vitreous humors. Semi-quantitative determination of VEGF-A and VEGF-B levels in fibrovascular proliferative membranes was performed using immunohistochemical staining. The results indicated that in the drug group, the levels of VEGF-A in the aqueous humor of patients prior to and after conbercept injection were 197.66±48.00 and 3.39±2.54 pg/ml, respectively. The levels of VEGF-A in the vitreous humor of patients in the surgical and drug groups were 267.53±179.60 and 21.43±5.81 pg/ml after injection, respectively. The levels of VEGF-B in the aqueous humor of patients prior to and after conbercept injection were 13.66±3.30 (before injection) and 2.17±0.94 pg/ml (after injection), respectively. The levels of VEGF-B in the vitreous humor of patients in the surgical and drug groups were 127.36±16.72 and 18.56±9.82 pg/ml after injection, respectively (P<0.05). Furthermore, in the drug group, the surgery time, bleeding and capillary formation were significantly reduced compared with those in the surgical group. Taken together, these results suggested that the levels of VEGF-A and -B decreased significantly in the aqueous humor of patients with PDR after conbercept injection. Furthermore, the levels of VEGF-A and -B in the vitreous humor of patients dropped significantly in the drug group compared with those in the surgical group. These results provide theoretical clinical support for the preoperative application of conbercept for patients with PDR. D.A. Spandidos 2021-04 2021-02-08 /pmc/articles/PMC7903486/ /pubmed/33732305 http://dx.doi.org/10.3892/etm.2021.9763 Text en Copyright: © Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zhang, Yunda Gao, Zhao Zhang, Ximei Yuan, Zhigang Ma, Tao Li, Gaiyun Gao, Xiaohong Effect of intravitreal conbercept injection on VEGF-A and -B levels in the aqueous and vitreous humor of patients with proliferative diabetic retinopathy |
title | Effect of intravitreal conbercept injection on VEGF-A and -B levels in the aqueous and vitreous humor of patients with proliferative diabetic retinopathy |
title_full | Effect of intravitreal conbercept injection on VEGF-A and -B levels in the aqueous and vitreous humor of patients with proliferative diabetic retinopathy |
title_fullStr | Effect of intravitreal conbercept injection on VEGF-A and -B levels in the aqueous and vitreous humor of patients with proliferative diabetic retinopathy |
title_full_unstemmed | Effect of intravitreal conbercept injection on VEGF-A and -B levels in the aqueous and vitreous humor of patients with proliferative diabetic retinopathy |
title_short | Effect of intravitreal conbercept injection on VEGF-A and -B levels in the aqueous and vitreous humor of patients with proliferative diabetic retinopathy |
title_sort | effect of intravitreal conbercept injection on vegf-a and -b levels in the aqueous and vitreous humor of patients with proliferative diabetic retinopathy |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903486/ https://www.ncbi.nlm.nih.gov/pubmed/33732305 http://dx.doi.org/10.3892/etm.2021.9763 |
work_keys_str_mv | AT zhangyunda effectofintravitrealconberceptinjectiononvegfaandblevelsintheaqueousandvitreoushumorofpatientswithproliferativediabeticretinopathy AT gaozhao effectofintravitrealconberceptinjectiononvegfaandblevelsintheaqueousandvitreoushumorofpatientswithproliferativediabeticretinopathy AT zhangximei effectofintravitrealconberceptinjectiononvegfaandblevelsintheaqueousandvitreoushumorofpatientswithproliferativediabeticretinopathy AT yuanzhigang effectofintravitrealconberceptinjectiononvegfaandblevelsintheaqueousandvitreoushumorofpatientswithproliferativediabeticretinopathy AT matao effectofintravitrealconberceptinjectiononvegfaandblevelsintheaqueousandvitreoushumorofpatientswithproliferativediabeticretinopathy AT ligaiyun effectofintravitrealconberceptinjectiononvegfaandblevelsintheaqueousandvitreoushumorofpatientswithproliferativediabeticretinopathy AT gaoxiaohong effectofintravitrealconberceptinjectiononvegfaandblevelsintheaqueousandvitreoushumorofpatientswithproliferativediabeticretinopathy |