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Analysis of microRNA expression profiles in porcine PBMCs after LPS stimulation
In the present study, we used microRNA (miRNA) sequencing to discover and explore the expression profiles of known and novel miRNAs in 1000 ng/ml LPS stimulated for 8 h vis-à-vis non-stimulated (i.e. control) PBMCs isolated from the blood of healthy pigs. A total of 291 known miRNAs were bio-computa...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903524/ https://www.ncbi.nlm.nih.gov/pubmed/31969027 http://dx.doi.org/10.1177/1753425920901560 |
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author | Zhang, Jing Xu, Xin Huang, Xingfa Zhu, Huiling Chen, Hongbo Wang, Wenjun Liu, Yulan |
author_facet | Zhang, Jing Xu, Xin Huang, Xingfa Zhu, Huiling Chen, Hongbo Wang, Wenjun Liu, Yulan |
author_sort | Zhang, Jing |
collection | PubMed |
description | In the present study, we used microRNA (miRNA) sequencing to discover and explore the expression profiles of known and novel miRNAs in 1000 ng/ml LPS stimulated for 8 h vis-à-vis non-stimulated (i.e. control) PBMCs isolated from the blood of healthy pigs. A total of 291 known miRNAs were bio-computationally identified in porcine PBMCs, and 228 novel miRNAs (not enlisted in the swine mirBase) were identified. Among these miRNAs, ssc-miR-148a-3p, ssc-let-7g, ssc-let-7f, 3_8760, ssc-miR-26a, ssc-miR-451, ssc-miR-21, ssc-miR-30d, ssc-miR-99a and ssc-miR-103 were the top 10 most abundant miRNAs in porcine PBMCs. Through miRNA differential analysis combined with quantitative PCR, we found the expressions of ssc-miR-122, ssc-miR-129b, ssc-miR-17-5p and ssc-miR-152 were significantly changed in porcine PBMCs after LPS stimulation. Furthermore, targets prediction and function analysis indicated a significant enrichment in gene ontology functional categories related to diseases, immunity and inflammation. In conclusion, this study on profiling of miRNAs expressed in LPS-stimulated PBMCs provides an important reference point for future studies on regulatory roles of miRNAs in porcine immune system. |
format | Online Article Text |
id | pubmed-7903524 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-79035242021-03-18 Analysis of microRNA expression profiles in porcine PBMCs after LPS stimulation Zhang, Jing Xu, Xin Huang, Xingfa Zhu, Huiling Chen, Hongbo Wang, Wenjun Liu, Yulan Innate Immun Original Articles In the present study, we used microRNA (miRNA) sequencing to discover and explore the expression profiles of known and novel miRNAs in 1000 ng/ml LPS stimulated for 8 h vis-à-vis non-stimulated (i.e. control) PBMCs isolated from the blood of healthy pigs. A total of 291 known miRNAs were bio-computationally identified in porcine PBMCs, and 228 novel miRNAs (not enlisted in the swine mirBase) were identified. Among these miRNAs, ssc-miR-148a-3p, ssc-let-7g, ssc-let-7f, 3_8760, ssc-miR-26a, ssc-miR-451, ssc-miR-21, ssc-miR-30d, ssc-miR-99a and ssc-miR-103 were the top 10 most abundant miRNAs in porcine PBMCs. Through miRNA differential analysis combined with quantitative PCR, we found the expressions of ssc-miR-122, ssc-miR-129b, ssc-miR-17-5p and ssc-miR-152 were significantly changed in porcine PBMCs after LPS stimulation. Furthermore, targets prediction and function analysis indicated a significant enrichment in gene ontology functional categories related to diseases, immunity and inflammation. In conclusion, this study on profiling of miRNAs expressed in LPS-stimulated PBMCs provides an important reference point for future studies on regulatory roles of miRNAs in porcine immune system. SAGE Publications 2020-01-22 2020-07 /pmc/articles/PMC7903524/ /pubmed/31969027 http://dx.doi.org/10.1177/1753425920901560 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Articles Zhang, Jing Xu, Xin Huang, Xingfa Zhu, Huiling Chen, Hongbo Wang, Wenjun Liu, Yulan Analysis of microRNA expression profiles in porcine PBMCs after LPS stimulation |
title | Analysis of microRNA expression profiles in porcine PBMCs after LPS
stimulation |
title_full | Analysis of microRNA expression profiles in porcine PBMCs after LPS
stimulation |
title_fullStr | Analysis of microRNA expression profiles in porcine PBMCs after LPS
stimulation |
title_full_unstemmed | Analysis of microRNA expression profiles in porcine PBMCs after LPS
stimulation |
title_short | Analysis of microRNA expression profiles in porcine PBMCs after LPS
stimulation |
title_sort | analysis of microrna expression profiles in porcine pbmcs after lps
stimulation |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903524/ https://www.ncbi.nlm.nih.gov/pubmed/31969027 http://dx.doi.org/10.1177/1753425920901560 |
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