Cargando…

ABAP1 Plays a Role in the Differentiation of Male and Female Gametes in Arabidopsis thaliana

The correct development of a diploid sporophyte body and a haploid gametophyte relies on a strict coordination between cell divisions in space and time. During plant reproduction, these divisions have to be temporally and spatially coordinated with cell differentiation processes, to ensure a success...

Descripción completa

Detalles Bibliográficos
Autores principales: Cabral, Luiz M., Masuda, Hana P., Ballesteros, Helkin F., de Almeida-Engler, Janice, Alves-Ferreira, Márcio, De Toni, Karen L. G., Bizotto, Fernanda M., Ferreira, Paulo C. G., Hemerly, Adriana S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903899/
https://www.ncbi.nlm.nih.gov/pubmed/33643370
http://dx.doi.org/10.3389/fpls.2021.642758
_version_ 1783654823802961920
author Cabral, Luiz M.
Masuda, Hana P.
Ballesteros, Helkin F.
de Almeida-Engler, Janice
Alves-Ferreira, Márcio
De Toni, Karen L. G.
Bizotto, Fernanda M.
Ferreira, Paulo C. G.
Hemerly, Adriana S.
author_facet Cabral, Luiz M.
Masuda, Hana P.
Ballesteros, Helkin F.
de Almeida-Engler, Janice
Alves-Ferreira, Márcio
De Toni, Karen L. G.
Bizotto, Fernanda M.
Ferreira, Paulo C. G.
Hemerly, Adriana S.
author_sort Cabral, Luiz M.
collection PubMed
description The correct development of a diploid sporophyte body and a haploid gametophyte relies on a strict coordination between cell divisions in space and time. During plant reproduction, these divisions have to be temporally and spatially coordinated with cell differentiation processes, to ensure a successful fertilization. Armadillo BTB Arabidopsis protein 1 (ABAP1) is a plant exclusive protein that has been previously reported to control proliferative cell divisions during leaf growth in Arabidopsis. Here, we show that ABAP1 binds to different transcription factors that regulate male and female gametophyte differentiation, repressing their target genes expression. During male gametogenesis, the ABAP1-TCP16 complex represses CDT1b transcription, and consequently regulates microspore first asymmetric mitosis. In the female gametogenesis, the ABAP1-ADAP complex represses EDA24-like transcription, regulating polar nuclei fusion to form the central cell. Therefore, besides its function during vegetative development, this work shows that ABAP1 is also involved in differentiation processes during plant reproduction, by having a dual role in regulating both the first asymmetric cell division of male gametophyte and the cell differentiation (or cell fusion) of female gametophyte.
format Online
Article
Text
id pubmed-7903899
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-79038992021-02-25 ABAP1 Plays a Role in the Differentiation of Male and Female Gametes in Arabidopsis thaliana Cabral, Luiz M. Masuda, Hana P. Ballesteros, Helkin F. de Almeida-Engler, Janice Alves-Ferreira, Márcio De Toni, Karen L. G. Bizotto, Fernanda M. Ferreira, Paulo C. G. Hemerly, Adriana S. Front Plant Sci Plant Science The correct development of a diploid sporophyte body and a haploid gametophyte relies on a strict coordination between cell divisions in space and time. During plant reproduction, these divisions have to be temporally and spatially coordinated with cell differentiation processes, to ensure a successful fertilization. Armadillo BTB Arabidopsis protein 1 (ABAP1) is a plant exclusive protein that has been previously reported to control proliferative cell divisions during leaf growth in Arabidopsis. Here, we show that ABAP1 binds to different transcription factors that regulate male and female gametophyte differentiation, repressing their target genes expression. During male gametogenesis, the ABAP1-TCP16 complex represses CDT1b transcription, and consequently regulates microspore first asymmetric mitosis. In the female gametogenesis, the ABAP1-ADAP complex represses EDA24-like transcription, regulating polar nuclei fusion to form the central cell. Therefore, besides its function during vegetative development, this work shows that ABAP1 is also involved in differentiation processes during plant reproduction, by having a dual role in regulating both the first asymmetric cell division of male gametophyte and the cell differentiation (or cell fusion) of female gametophyte. Frontiers Media S.A. 2021-02-10 /pmc/articles/PMC7903899/ /pubmed/33643370 http://dx.doi.org/10.3389/fpls.2021.642758 Text en Copyright © 2021 Cabral, Masuda, Ballesteros, de Almeida-Engler, Alves-Ferreira, De Toni, Bizotto, Ferreira and Hemerly. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Cabral, Luiz M.
Masuda, Hana P.
Ballesteros, Helkin F.
de Almeida-Engler, Janice
Alves-Ferreira, Márcio
De Toni, Karen L. G.
Bizotto, Fernanda M.
Ferreira, Paulo C. G.
Hemerly, Adriana S.
ABAP1 Plays a Role in the Differentiation of Male and Female Gametes in Arabidopsis thaliana
title ABAP1 Plays a Role in the Differentiation of Male and Female Gametes in Arabidopsis thaliana
title_full ABAP1 Plays a Role in the Differentiation of Male and Female Gametes in Arabidopsis thaliana
title_fullStr ABAP1 Plays a Role in the Differentiation of Male and Female Gametes in Arabidopsis thaliana
title_full_unstemmed ABAP1 Plays a Role in the Differentiation of Male and Female Gametes in Arabidopsis thaliana
title_short ABAP1 Plays a Role in the Differentiation of Male and Female Gametes in Arabidopsis thaliana
title_sort abap1 plays a role in the differentiation of male and female gametes in arabidopsis thaliana
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903899/
https://www.ncbi.nlm.nih.gov/pubmed/33643370
http://dx.doi.org/10.3389/fpls.2021.642758
work_keys_str_mv AT cabralluizm abap1playsaroleinthedifferentiationofmaleandfemalegametesinarabidopsisthaliana
AT masudahanap abap1playsaroleinthedifferentiationofmaleandfemalegametesinarabidopsisthaliana
AT ballesteroshelkinf abap1playsaroleinthedifferentiationofmaleandfemalegametesinarabidopsisthaliana
AT dealmeidaenglerjanice abap1playsaroleinthedifferentiationofmaleandfemalegametesinarabidopsisthaliana
AT alvesferreiramarcio abap1playsaroleinthedifferentiationofmaleandfemalegametesinarabidopsisthaliana
AT detonikarenlg abap1playsaroleinthedifferentiationofmaleandfemalegametesinarabidopsisthaliana
AT bizottofernandam abap1playsaroleinthedifferentiationofmaleandfemalegametesinarabidopsisthaliana
AT ferreirapaulocg abap1playsaroleinthedifferentiationofmaleandfemalegametesinarabidopsisthaliana
AT hemerlyadrianas abap1playsaroleinthedifferentiationofmaleandfemalegametesinarabidopsisthaliana