Monitoring Schizosaccharomyces pombe genome stress by visualizing end-binding protein Ku

Studies of genome stability have exploited visualization of fluorescently tagged proteins in live cells to characterize DNA damage, checkpoint, and repair responses. In this report, we describe a new tool for fission yeast, a tagged version of the end-binding protein Pku70 which is part of the KU pr...

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Detalles Bibliográficos
Autores principales: Jones, Chance E., Forsburg, Susan L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2021
Materias:
Methods & Techniques
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904001/
https://www.ncbi.nlm.nih.gov/pubmed/33579693
http://dx.doi.org/10.1242/bio.054346
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author Jones, Chance E.
Forsburg, Susan L.
author_facet Jones, Chance E.
Forsburg, Susan L.
author_sort Jones, Chance E.
collection PubMed
description Studies of genome stability have exploited visualization of fluorescently tagged proteins in live cells to characterize DNA damage, checkpoint, and repair responses. In this report, we describe a new tool for fission yeast, a tagged version of the end-binding protein Pku70 which is part of the KU protein complex. We compare Pku70 localization to other markers upon treatment to various genotoxins, and identify a unique pattern of distribution. Pku70 provides a new tool to define and characterize DNA lesions and the repair response.
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spelling pubmed-79040012021-02-25 Monitoring Schizosaccharomyces pombe genome stress by visualizing end-binding protein Ku Jones, Chance E. Forsburg, Susan L. Biol Open Methods & Techniques Studies of genome stability have exploited visualization of fluorescently tagged proteins in live cells to characterize DNA damage, checkpoint, and repair responses. In this report, we describe a new tool for fission yeast, a tagged version of the end-binding protein Pku70 which is part of the KU protein complex. We compare Pku70 localization to other markers upon treatment to various genotoxins, and identify a unique pattern of distribution. Pku70 provides a new tool to define and characterize DNA lesions and the repair response. The Company of Biologists Ltd 2021-02-15 /pmc/articles/PMC7904001/ /pubmed/33579693 http://dx.doi.org/10.1242/bio.054346 Text en © 2021. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Methods & Techniques
Jones, Chance E.
Forsburg, Susan L.
Monitoring Schizosaccharomyces pombe genome stress by visualizing end-binding protein Ku
title Monitoring Schizosaccharomyces pombe genome stress by visualizing end-binding protein Ku
title_full Monitoring Schizosaccharomyces pombe genome stress by visualizing end-binding protein Ku
title_fullStr Monitoring Schizosaccharomyces pombe genome stress by visualizing end-binding protein Ku
title_full_unstemmed Monitoring Schizosaccharomyces pombe genome stress by visualizing end-binding protein Ku
title_short Monitoring Schizosaccharomyces pombe genome stress by visualizing end-binding protein Ku
title_sort monitoring schizosaccharomyces pombe genome stress by visualizing end-binding protein ku
topic Methods & Techniques
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904001/
https://www.ncbi.nlm.nih.gov/pubmed/33579693
http://dx.doi.org/10.1242/bio.054346
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