Cargando…

Vasohibin 1 selectively regulates secondary sprouting and lymphangiogenesis in the zebrafish trunk

Previous studies have shown that Vasohibin 1 (Vash1) is stimulated by VEGFs in endothelial cells and that its overexpression interferes with angiogenesis in vivo. Recently, Vash1 was found to mediate tubulin detyrosination, a post-translational modification that is implicated in many cell functions,...

Descripción completa

Detalles Bibliográficos
Autores principales: de Oliveira, Marta Bastos, Meier, Katja, Jung, Simone, Bartels-Klein, Eireen, Coxam, Baptiste, Geudens, Ilse, Szymborska, Anna, Skoczylas, Renae, Fechner, Ines, Koltowska, Katarzyna, Gerhardt, Holger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904002/
https://www.ncbi.nlm.nih.gov/pubmed/33547133
http://dx.doi.org/10.1242/dev.194993
_version_ 1783654844041527296
author de Oliveira, Marta Bastos
Meier, Katja
Jung, Simone
Bartels-Klein, Eireen
Coxam, Baptiste
Geudens, Ilse
Szymborska, Anna
Skoczylas, Renae
Fechner, Ines
Koltowska, Katarzyna
Gerhardt, Holger
author_facet de Oliveira, Marta Bastos
Meier, Katja
Jung, Simone
Bartels-Klein, Eireen
Coxam, Baptiste
Geudens, Ilse
Szymborska, Anna
Skoczylas, Renae
Fechner, Ines
Koltowska, Katarzyna
Gerhardt, Holger
author_sort de Oliveira, Marta Bastos
collection PubMed
description Previous studies have shown that Vasohibin 1 (Vash1) is stimulated by VEGFs in endothelial cells and that its overexpression interferes with angiogenesis in vivo. Recently, Vash1 was found to mediate tubulin detyrosination, a post-translational modification that is implicated in many cell functions, such as cell division. Here, we used the zebrafish embryo to investigate the cellular and subcellular mechanisms of Vash1 on endothelial microtubules during formation of the trunk vasculature. We show that microtubules within venous-derived secondary sprouts are strongly and selectively detyrosinated in comparison with other endothelial cells, and that this difference is lost upon vash1 knockdown. Vash1 depletion in zebrafish specifically affected secondary sprouting from the posterior cardinal vein, increasing endothelial cell divisions and cell number in the sprouts. We show that altering secondary sprout numbers and structure upon Vash1 depletion leads to defective lymphatic vessel formation and ectopic lymphatic progenitor specification in the zebrafish trunk.
format Online
Article
Text
id pubmed-7904002
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher The Company of Biologists Ltd
record_format MEDLINE/PubMed
spelling pubmed-79040022021-03-02 Vasohibin 1 selectively regulates secondary sprouting and lymphangiogenesis in the zebrafish trunk de Oliveira, Marta Bastos Meier, Katja Jung, Simone Bartels-Klein, Eireen Coxam, Baptiste Geudens, Ilse Szymborska, Anna Skoczylas, Renae Fechner, Ines Koltowska, Katarzyna Gerhardt, Holger Development Research Article Previous studies have shown that Vasohibin 1 (Vash1) is stimulated by VEGFs in endothelial cells and that its overexpression interferes with angiogenesis in vivo. Recently, Vash1 was found to mediate tubulin detyrosination, a post-translational modification that is implicated in many cell functions, such as cell division. Here, we used the zebrafish embryo to investigate the cellular and subcellular mechanisms of Vash1 on endothelial microtubules during formation of the trunk vasculature. We show that microtubules within venous-derived secondary sprouts are strongly and selectively detyrosinated in comparison with other endothelial cells, and that this difference is lost upon vash1 knockdown. Vash1 depletion in zebrafish specifically affected secondary sprouting from the posterior cardinal vein, increasing endothelial cell divisions and cell number in the sprouts. We show that altering secondary sprout numbers and structure upon Vash1 depletion leads to defective lymphatic vessel formation and ectopic lymphatic progenitor specification in the zebrafish trunk. The Company of Biologists Ltd 2021-02-19 /pmc/articles/PMC7904002/ /pubmed/33547133 http://dx.doi.org/10.1242/dev.194993 Text en © 2021. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
de Oliveira, Marta Bastos
Meier, Katja
Jung, Simone
Bartels-Klein, Eireen
Coxam, Baptiste
Geudens, Ilse
Szymborska, Anna
Skoczylas, Renae
Fechner, Ines
Koltowska, Katarzyna
Gerhardt, Holger
Vasohibin 1 selectively regulates secondary sprouting and lymphangiogenesis in the zebrafish trunk
title Vasohibin 1 selectively regulates secondary sprouting and lymphangiogenesis in the zebrafish trunk
title_full Vasohibin 1 selectively regulates secondary sprouting and lymphangiogenesis in the zebrafish trunk
title_fullStr Vasohibin 1 selectively regulates secondary sprouting and lymphangiogenesis in the zebrafish trunk
title_full_unstemmed Vasohibin 1 selectively regulates secondary sprouting and lymphangiogenesis in the zebrafish trunk
title_short Vasohibin 1 selectively regulates secondary sprouting and lymphangiogenesis in the zebrafish trunk
title_sort vasohibin 1 selectively regulates secondary sprouting and lymphangiogenesis in the zebrafish trunk
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904002/
https://www.ncbi.nlm.nih.gov/pubmed/33547133
http://dx.doi.org/10.1242/dev.194993
work_keys_str_mv AT deoliveiramartabastos vasohibin1selectivelyregulatessecondarysproutingandlymphangiogenesisinthezebrafishtrunk
AT meierkatja vasohibin1selectivelyregulatessecondarysproutingandlymphangiogenesisinthezebrafishtrunk
AT jungsimone vasohibin1selectivelyregulatessecondarysproutingandlymphangiogenesisinthezebrafishtrunk
AT bartelskleineireen vasohibin1selectivelyregulatessecondarysproutingandlymphangiogenesisinthezebrafishtrunk
AT coxambaptiste vasohibin1selectivelyregulatessecondarysproutingandlymphangiogenesisinthezebrafishtrunk
AT geudensilse vasohibin1selectivelyregulatessecondarysproutingandlymphangiogenesisinthezebrafishtrunk
AT szymborskaanna vasohibin1selectivelyregulatessecondarysproutingandlymphangiogenesisinthezebrafishtrunk
AT skoczylasrenae vasohibin1selectivelyregulatessecondarysproutingandlymphangiogenesisinthezebrafishtrunk
AT fechnerines vasohibin1selectivelyregulatessecondarysproutingandlymphangiogenesisinthezebrafishtrunk
AT koltowskakatarzyna vasohibin1selectivelyregulatessecondarysproutingandlymphangiogenesisinthezebrafishtrunk
AT gerhardtholger vasohibin1selectivelyregulatessecondarysproutingandlymphangiogenesisinthezebrafishtrunk