Cargando…

The effect of sodium selenite on apoptotic gene expression and development of in vitro cultured mouse oocytes in comparison with in vivo obtained oocytes

In vitro maturation (IVM) of oocytes is widely used in assisted reproduction technologies. The present study aimed to improve the in vitro oocyte maturation and its development through enriching the culture media with sodium selenite (SS). Moreover, the effects of SS on the expression of the oocytes...

Descripción completa

Detalles Bibliográficos
Autores principales: Malekzadeh Kebria, Maziar, Salehnia, Mojdeh, Zavareh, Saeed, Moazzeni, Seyyed Saeed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Urmia University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904118/
https://www.ncbi.nlm.nih.gov/pubmed/33643591
http://dx.doi.org/10.30466/vrf.2018.93471.2255
_version_ 1783654862561476608
author Malekzadeh Kebria, Maziar
Salehnia, Mojdeh
Zavareh, Saeed
Moazzeni, Seyyed Saeed
author_facet Malekzadeh Kebria, Maziar
Salehnia, Mojdeh
Zavareh, Saeed
Moazzeni, Seyyed Saeed
author_sort Malekzadeh Kebria, Maziar
collection PubMed
description In vitro maturation (IVM) of oocytes is widely used in assisted reproduction technologies. The present study aimed to improve the in vitro oocyte maturation and its development through enriching the culture media with sodium selenite (SS). Moreover, the effects of SS on the expression of the oocytes apoptosis-related genes were assessed. In this study, male and female NMRI mice were used and after collecting their germinal vesicle (GV) oocytes, they were cultured with SS (experimental group) and without SS (control group). Collected metaphase II oocytes (MII) from the fallopian tube were considered as in vivo group. After in vitro culture, the oocytes were assessed in terms of nuclear maturation. The MII oocytes were inseminated and the development was examined until the blastocyst stage. Also, oocytes were subjected to the molecular analysis for evaluating the expression of BAX, BCL2, P53, and BAD genes using the real-time RT-PCR. The maturation rate was significantly increased in the SS supplemented group compared to the control one. The developmental rate of the embryos was significantly higher for both of the in vivo and SS supplemented groups rather than the control one, however, no significant difference was seen between these rates of the experimental and in vivo groups. Real-time RT-PCR did not show any significant differences in the expression of the apoptosis-related genes for all of the studied groups. The p53 gene was not expressed in any of groups. Sodium selenite improved the oocyte developmental competence but did not change the expression of the apoptosis-related genes in MII oocytes.
format Online
Article
Text
id pubmed-7904118
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Urmia University Press
record_format MEDLINE/PubMed
spelling pubmed-79041182021-02-25 The effect of sodium selenite on apoptotic gene expression and development of in vitro cultured mouse oocytes in comparison with in vivo obtained oocytes Malekzadeh Kebria, Maziar Salehnia, Mojdeh Zavareh, Saeed Moazzeni, Seyyed Saeed Vet Res Forum Original Article In vitro maturation (IVM) of oocytes is widely used in assisted reproduction technologies. The present study aimed to improve the in vitro oocyte maturation and its development through enriching the culture media with sodium selenite (SS). Moreover, the effects of SS on the expression of the oocytes apoptosis-related genes were assessed. In this study, male and female NMRI mice were used and after collecting their germinal vesicle (GV) oocytes, they were cultured with SS (experimental group) and without SS (control group). Collected metaphase II oocytes (MII) from the fallopian tube were considered as in vivo group. After in vitro culture, the oocytes were assessed in terms of nuclear maturation. The MII oocytes were inseminated and the development was examined until the blastocyst stage. Also, oocytes were subjected to the molecular analysis for evaluating the expression of BAX, BCL2, P53, and BAD genes using the real-time RT-PCR. The maturation rate was significantly increased in the SS supplemented group compared to the control one. The developmental rate of the embryos was significantly higher for both of the in vivo and SS supplemented groups rather than the control one, however, no significant difference was seen between these rates of the experimental and in vivo groups. Real-time RT-PCR did not show any significant differences in the expression of the apoptosis-related genes for all of the studied groups. The p53 gene was not expressed in any of groups. Sodium selenite improved the oocyte developmental competence but did not change the expression of the apoptosis-related genes in MII oocytes. Urmia University Press 2020 2020-12-15 /pmc/articles/PMC7904118/ /pubmed/33643591 http://dx.doi.org/10.30466/vrf.2018.93471.2255 Text en © 2020 Urmia University. All rights reserved This is an open-access article distributed under the terms of the Creative Commons Attribution-noncommercial 4.0 International License, (https://creativecommons.org/licenses/by-nc/4.0/) which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
Malekzadeh Kebria, Maziar
Salehnia, Mojdeh
Zavareh, Saeed
Moazzeni, Seyyed Saeed
The effect of sodium selenite on apoptotic gene expression and development of in vitro cultured mouse oocytes in comparison with in vivo obtained oocytes
title The effect of sodium selenite on apoptotic gene expression and development of in vitro cultured mouse oocytes in comparison with in vivo obtained oocytes
title_full The effect of sodium selenite on apoptotic gene expression and development of in vitro cultured mouse oocytes in comparison with in vivo obtained oocytes
title_fullStr The effect of sodium selenite on apoptotic gene expression and development of in vitro cultured mouse oocytes in comparison with in vivo obtained oocytes
title_full_unstemmed The effect of sodium selenite on apoptotic gene expression and development of in vitro cultured mouse oocytes in comparison with in vivo obtained oocytes
title_short The effect of sodium selenite on apoptotic gene expression and development of in vitro cultured mouse oocytes in comparison with in vivo obtained oocytes
title_sort effect of sodium selenite on apoptotic gene expression and development of in vitro cultured mouse oocytes in comparison with in vivo obtained oocytes
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904118/
https://www.ncbi.nlm.nih.gov/pubmed/33643591
http://dx.doi.org/10.30466/vrf.2018.93471.2255
work_keys_str_mv AT malekzadehkebriamaziar theeffectofsodiumseleniteonapoptoticgeneexpressionanddevelopmentofinvitroculturedmouseoocytesincomparisonwithinvivoobtainedoocytes
AT salehniamojdeh theeffectofsodiumseleniteonapoptoticgeneexpressionanddevelopmentofinvitroculturedmouseoocytesincomparisonwithinvivoobtainedoocytes
AT zavarehsaeed theeffectofsodiumseleniteonapoptoticgeneexpressionanddevelopmentofinvitroculturedmouseoocytesincomparisonwithinvivoobtainedoocytes
AT moazzeniseyyedsaeed theeffectofsodiumseleniteonapoptoticgeneexpressionanddevelopmentofinvitroculturedmouseoocytesincomparisonwithinvivoobtainedoocytes
AT malekzadehkebriamaziar effectofsodiumseleniteonapoptoticgeneexpressionanddevelopmentofinvitroculturedmouseoocytesincomparisonwithinvivoobtainedoocytes
AT salehniamojdeh effectofsodiumseleniteonapoptoticgeneexpressionanddevelopmentofinvitroculturedmouseoocytesincomparisonwithinvivoobtainedoocytes
AT zavarehsaeed effectofsodiumseleniteonapoptoticgeneexpressionanddevelopmentofinvitroculturedmouseoocytesincomparisonwithinvivoobtainedoocytes
AT moazzeniseyyedsaeed effectofsodiumseleniteonapoptoticgeneexpressionanddevelopmentofinvitroculturedmouseoocytesincomparisonwithinvivoobtainedoocytes