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The Effects of Human Bone Marrow-Derived Mesenchymal Stem Cell Conditioned Media Produced with Fetal Bovine Serum or Human Platelet Lysate on Skin Rejuvenation Characteristics

BACKGROUND AND OBJECTIVES: Human mesenchymal stem cell-conditioned medium (MSC-CM) is produced using mesenchymal stem cell culture technology and has various benefits for the skin, including wrinkle removal, skin regeneration, and increased antioxidant activity. Its popularity is thus increasing in...

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Autores principales: Kim, Si-Na, Lee, Chan-Ju, Nam, JeeHoon, Choi, Byeol, Chung, Eunkyung, Song, Sun Uk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Stem Cell Research 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904527/
https://www.ncbi.nlm.nih.gov/pubmed/33377452
http://dx.doi.org/10.15283/ijsc20070
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author Kim, Si-Na
Lee, Chan-Ju
Nam, JeeHoon
Choi, Byeol
Chung, Eunkyung
Song, Sun Uk
author_facet Kim, Si-Na
Lee, Chan-Ju
Nam, JeeHoon
Choi, Byeol
Chung, Eunkyung
Song, Sun Uk
author_sort Kim, Si-Na
collection PubMed
description BACKGROUND AND OBJECTIVES: Human mesenchymal stem cell-conditioned medium (MSC-CM) is produced using mesenchymal stem cell culture technology and has various benefits for the skin, including wrinkle removal, skin regeneration, and increased antioxidant activity. Its popularity is thus increasing in the field of functional cosmetics. METHODS AND RESULTS: In this study, we analyzed the effects of fetal bovine serum-supplemented MSC-CM (FBS- MSC-CM) and human platelet lysate-supplemented MSC-CM (hPL-MSC-CM) on skin rejuvenation characteristics. We found that the concentrations of important growth factors (VEGF, TGF-β1, and HGF) and secretory proteins for skin regeneration were significantly higher in hPL-MSC-CM than in FBS-MSC-CM. Furthermore, the capacity for inducing proliferation of human dermal fibroblast (HDF) and keratinocytes, the migration ability of HDF, extracellular matrix (ECM) production such as collagen and elastin was higher in hPL-MSC-CM than that in FBS- MSC-CM. CONCLUSIONS: These results support the usefulness and high economic value of hPL-MSC-CM as an alternative source of FBS-MSC-CM in the cosmetic industry for skin rejuvenation.
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spelling pubmed-79045272021-03-03 The Effects of Human Bone Marrow-Derived Mesenchymal Stem Cell Conditioned Media Produced with Fetal Bovine Serum or Human Platelet Lysate on Skin Rejuvenation Characteristics Kim, Si-Na Lee, Chan-Ju Nam, JeeHoon Choi, Byeol Chung, Eunkyung Song, Sun Uk Int J Stem Cells Original Article BACKGROUND AND OBJECTIVES: Human mesenchymal stem cell-conditioned medium (MSC-CM) is produced using mesenchymal stem cell culture technology and has various benefits for the skin, including wrinkle removal, skin regeneration, and increased antioxidant activity. Its popularity is thus increasing in the field of functional cosmetics. METHODS AND RESULTS: In this study, we analyzed the effects of fetal bovine serum-supplemented MSC-CM (FBS- MSC-CM) and human platelet lysate-supplemented MSC-CM (hPL-MSC-CM) on skin rejuvenation characteristics. We found that the concentrations of important growth factors (VEGF, TGF-β1, and HGF) and secretory proteins for skin regeneration were significantly higher in hPL-MSC-CM than in FBS-MSC-CM. Furthermore, the capacity for inducing proliferation of human dermal fibroblast (HDF) and keratinocytes, the migration ability of HDF, extracellular matrix (ECM) production such as collagen and elastin was higher in hPL-MSC-CM than that in FBS- MSC-CM. CONCLUSIONS: These results support the usefulness and high economic value of hPL-MSC-CM as an alternative source of FBS-MSC-CM in the cosmetic industry for skin rejuvenation. Korean Society for Stem Cell Research 2020-12-31 /pmc/articles/PMC7904527/ /pubmed/33377452 http://dx.doi.org/10.15283/ijsc20070 Text en Copyright © 2021 by the Korean Society for Stem Cell Research This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Si-Na
Lee, Chan-Ju
Nam, JeeHoon
Choi, Byeol
Chung, Eunkyung
Song, Sun Uk
The Effects of Human Bone Marrow-Derived Mesenchymal Stem Cell Conditioned Media Produced with Fetal Bovine Serum or Human Platelet Lysate on Skin Rejuvenation Characteristics
title The Effects of Human Bone Marrow-Derived Mesenchymal Stem Cell Conditioned Media Produced with Fetal Bovine Serum or Human Platelet Lysate on Skin Rejuvenation Characteristics
title_full The Effects of Human Bone Marrow-Derived Mesenchymal Stem Cell Conditioned Media Produced with Fetal Bovine Serum or Human Platelet Lysate on Skin Rejuvenation Characteristics
title_fullStr The Effects of Human Bone Marrow-Derived Mesenchymal Stem Cell Conditioned Media Produced with Fetal Bovine Serum or Human Platelet Lysate on Skin Rejuvenation Characteristics
title_full_unstemmed The Effects of Human Bone Marrow-Derived Mesenchymal Stem Cell Conditioned Media Produced with Fetal Bovine Serum or Human Platelet Lysate on Skin Rejuvenation Characteristics
title_short The Effects of Human Bone Marrow-Derived Mesenchymal Stem Cell Conditioned Media Produced with Fetal Bovine Serum or Human Platelet Lysate on Skin Rejuvenation Characteristics
title_sort effects of human bone marrow-derived mesenchymal stem cell conditioned media produced with fetal bovine serum or human platelet lysate on skin rejuvenation characteristics
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904527/
https://www.ncbi.nlm.nih.gov/pubmed/33377452
http://dx.doi.org/10.15283/ijsc20070
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