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Endosperm cell size reduction caused by osmotic adjustment during nighttime warming in rice
High night temperature (HNT) often reduces yield in field crops. In rice, HNT during the ripening stage diminishes endosperm cell size, resulting in a considerable reduction in final kernel weight; however, little is known about the underlying mechanisms at cell level. In this study, we performed pi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904791/ https://www.ncbi.nlm.nih.gov/pubmed/33627723 http://dx.doi.org/10.1038/s41598-021-83870-1 |
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author | Wada, Hiroshi Chang, Fang-Yu Hatakeyama, Yuto Erra-Balsells, Rosa Araki, Takuya Nakano, Hiroshi Nonami, Hiroshi |
author_facet | Wada, Hiroshi Chang, Fang-Yu Hatakeyama, Yuto Erra-Balsells, Rosa Araki, Takuya Nakano, Hiroshi Nonami, Hiroshi |
author_sort | Wada, Hiroshi |
collection | PubMed |
description | High night temperature (HNT) often reduces yield in field crops. In rice, HNT during the ripening stage diminishes endosperm cell size, resulting in a considerable reduction in final kernel weight; however, little is known about the underlying mechanisms at cell level. In this study, we performed picolitre pressure-probe-electrospray-ionization mass spectrometry to directly determine metabolites in growing inner endosperm cells of intact seeds produced under HNT conditions, combining with (13)C feeding and water status measurements including in situ turgor assay. Microscopic observation in the inner zone suggested that approximately 24.2% of decrease in cell expansion rate occurred under HNT at early ripening stage, leading to a reduction in cell volume. It has been shown that HNT-treated plants were subjected to mild shoot water deficit at night and endosperm cell turgor was sustained by a decline in osmotic potential. Cell metabolomics also suggests that active solute accumulation was caused by a partial inhibition of wall and starch biosynthesis under HNT conditions. Because metabolites were detected in the single cells, it is concluded that a partial arrest of cell expansion observed in the inner endosperms was caused by osmotic adjustment at mild water deficit during HNT conditions. |
format | Online Article Text |
id | pubmed-7904791 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-79047912021-02-25 Endosperm cell size reduction caused by osmotic adjustment during nighttime warming in rice Wada, Hiroshi Chang, Fang-Yu Hatakeyama, Yuto Erra-Balsells, Rosa Araki, Takuya Nakano, Hiroshi Nonami, Hiroshi Sci Rep Article High night temperature (HNT) often reduces yield in field crops. In rice, HNT during the ripening stage diminishes endosperm cell size, resulting in a considerable reduction in final kernel weight; however, little is known about the underlying mechanisms at cell level. In this study, we performed picolitre pressure-probe-electrospray-ionization mass spectrometry to directly determine metabolites in growing inner endosperm cells of intact seeds produced under HNT conditions, combining with (13)C feeding and water status measurements including in situ turgor assay. Microscopic observation in the inner zone suggested that approximately 24.2% of decrease in cell expansion rate occurred under HNT at early ripening stage, leading to a reduction in cell volume. It has been shown that HNT-treated plants were subjected to mild shoot water deficit at night and endosperm cell turgor was sustained by a decline in osmotic potential. Cell metabolomics also suggests that active solute accumulation was caused by a partial inhibition of wall and starch biosynthesis under HNT conditions. Because metabolites were detected in the single cells, it is concluded that a partial arrest of cell expansion observed in the inner endosperms was caused by osmotic adjustment at mild water deficit during HNT conditions. Nature Publishing Group UK 2021-02-24 /pmc/articles/PMC7904791/ /pubmed/33627723 http://dx.doi.org/10.1038/s41598-021-83870-1 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Wada, Hiroshi Chang, Fang-Yu Hatakeyama, Yuto Erra-Balsells, Rosa Araki, Takuya Nakano, Hiroshi Nonami, Hiroshi Endosperm cell size reduction caused by osmotic adjustment during nighttime warming in rice |
title | Endosperm cell size reduction caused by osmotic adjustment during nighttime warming in rice |
title_full | Endosperm cell size reduction caused by osmotic adjustment during nighttime warming in rice |
title_fullStr | Endosperm cell size reduction caused by osmotic adjustment during nighttime warming in rice |
title_full_unstemmed | Endosperm cell size reduction caused by osmotic adjustment during nighttime warming in rice |
title_short | Endosperm cell size reduction caused by osmotic adjustment during nighttime warming in rice |
title_sort | endosperm cell size reduction caused by osmotic adjustment during nighttime warming in rice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904791/ https://www.ncbi.nlm.nih.gov/pubmed/33627723 http://dx.doi.org/10.1038/s41598-021-83870-1 |
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