Identification of microalgae cultured in Bold’s Basal medium from freshwater samples, from a high-rise city

This study aimed at exploring microalgal heterogeneity from fresh water samples collected from inland water bodies in the heavily built city of Singapore. Culturable pure isolates (n = 94) were subject to an in-house microalgal DNA extraction method and LSU rDNA sequencing. Isolates were analysed fo...

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Detalles Bibliográficos
Autores principales: Lloyd, Charmaine, Tan, Kai Heng, Lim, Kar Leong, Valu, Vimala Gana, Fun, Sarah Mei Ying, Chye, Teng Rong, Mak, Hui Min, Sim, Wei Xiong, Musa, Sarah  Liyana, Ng, Joscelyn Jun Quan, Bte Nordin, Nazurah Syazana, Bte Md Aidzil, Nurhazlyn, Eng, Zephyr Yu Wen, Manickavasagam, Punithavathy, New, Jen Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904821/
https://www.ncbi.nlm.nih.gov/pubmed/33627771
http://dx.doi.org/10.1038/s41598-021-84112-0
Descripción
Sumario:This study aimed at exploring microalgal heterogeneity from fresh water samples collected from inland water bodies in the heavily built city of Singapore. Culturable pure isolates (n = 94) were subject to an in-house microalgal DNA extraction method and LSU rDNA sequencing. Isolates were analysed for their predominance and distribution. A total of 17 different algal genera were identified (H = 2.8, E(H) = 0.6), of which Scenedesmus spp. and Chlorella spp. constituted 27.5% and 21.3% of isolates respectively, followed by Micractinium spp. (18.8%) and Chlamydomonas spp. (12.5%). We also report 16 new microalgal strains from this region. The data is important from an ecological and biotechnological perspective.

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