Identification of microalgae cultured in Bold’s Basal medium from freshwater samples, from a high-rise city

This study aimed at exploring microalgal heterogeneity from fresh water samples collected from inland water bodies in the heavily built city of Singapore. Culturable pure isolates (n = 94) were subject to an in-house microalgal DNA extraction method and LSU rDNA sequencing. Isolates were analysed fo...

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Autores principales: Lloyd, Charmaine, Tan, Kai Heng, Lim, Kar Leong, Valu, Vimala Gana, Fun, Sarah Mei Ying, Chye, Teng Rong, Mak, Hui Min, Sim, Wei Xiong, Musa, Sarah  Liyana, Ng, Joscelyn Jun Quan, Bte Nordin, Nazurah Syazana, Bte Md Aidzil, Nurhazlyn, Eng, Zephyr Yu Wen, Manickavasagam, Punithavathy, New, Jen Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904821/
https://www.ncbi.nlm.nih.gov/pubmed/33627771
http://dx.doi.org/10.1038/s41598-021-84112-0
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author Lloyd, Charmaine
Tan, Kai Heng
Lim, Kar Leong
Valu, Vimala Gana
Fun, Sarah Mei Ying
Chye, Teng Rong
Mak, Hui Min
Sim, Wei Xiong
Musa, Sarah  Liyana
Ng, Joscelyn Jun Quan
Bte Nordin, Nazurah Syazana
Bte Md Aidzil, Nurhazlyn
Eng, Zephyr Yu Wen
Manickavasagam, Punithavathy
New, Jen Yan
author_facet Lloyd, Charmaine
Tan, Kai Heng
Lim, Kar Leong
Valu, Vimala Gana
Fun, Sarah Mei Ying
Chye, Teng Rong
Mak, Hui Min
Sim, Wei Xiong
Musa, Sarah  Liyana
Ng, Joscelyn Jun Quan
Bte Nordin, Nazurah Syazana
Bte Md Aidzil, Nurhazlyn
Eng, Zephyr Yu Wen
Manickavasagam, Punithavathy
New, Jen Yan
author_sort Lloyd, Charmaine
collection PubMed
description This study aimed at exploring microalgal heterogeneity from fresh water samples collected from inland water bodies in the heavily built city of Singapore. Culturable pure isolates (n = 94) were subject to an in-house microalgal DNA extraction method and LSU rDNA sequencing. Isolates were analysed for their predominance and distribution. A total of 17 different algal genera were identified (H = 2.8, E(H) = 0.6), of which Scenedesmus spp. and Chlorella spp. constituted 27.5% and 21.3% of isolates respectively, followed by Micractinium spp. (18.8%) and Chlamydomonas spp. (12.5%). We also report 16 new microalgal strains from this region. The data is important from an ecological and biotechnological perspective.
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spelling pubmed-79048212021-02-25 Identification of microalgae cultured in Bold’s Basal medium from freshwater samples, from a high-rise city Lloyd, Charmaine Tan, Kai Heng Lim, Kar Leong Valu, Vimala Gana Fun, Sarah Mei Ying Chye, Teng Rong Mak, Hui Min Sim, Wei Xiong Musa, Sarah  Liyana Ng, Joscelyn Jun Quan Bte Nordin, Nazurah Syazana Bte Md Aidzil, Nurhazlyn Eng, Zephyr Yu Wen Manickavasagam, Punithavathy New, Jen Yan Sci Rep Article This study aimed at exploring microalgal heterogeneity from fresh water samples collected from inland water bodies in the heavily built city of Singapore. Culturable pure isolates (n = 94) were subject to an in-house microalgal DNA extraction method and LSU rDNA sequencing. Isolates were analysed for their predominance and distribution. A total of 17 different algal genera were identified (H = 2.8, E(H) = 0.6), of which Scenedesmus spp. and Chlorella spp. constituted 27.5% and 21.3% of isolates respectively, followed by Micractinium spp. (18.8%) and Chlamydomonas spp. (12.5%). We also report 16 new microalgal strains from this region. The data is important from an ecological and biotechnological perspective. Nature Publishing Group UK 2021-02-24 /pmc/articles/PMC7904821/ /pubmed/33627771 http://dx.doi.org/10.1038/s41598-021-84112-0 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lloyd, Charmaine
Tan, Kai Heng
Lim, Kar Leong
Valu, Vimala Gana
Fun, Sarah Mei Ying
Chye, Teng Rong
Mak, Hui Min
Sim, Wei Xiong
Musa, Sarah  Liyana
Ng, Joscelyn Jun Quan
Bte Nordin, Nazurah Syazana
Bte Md Aidzil, Nurhazlyn
Eng, Zephyr Yu Wen
Manickavasagam, Punithavathy
New, Jen Yan
Identification of microalgae cultured in Bold’s Basal medium from freshwater samples, from a high-rise city
title Identification of microalgae cultured in Bold’s Basal medium from freshwater samples, from a high-rise city
title_full Identification of microalgae cultured in Bold’s Basal medium from freshwater samples, from a high-rise city
title_fullStr Identification of microalgae cultured in Bold’s Basal medium from freshwater samples, from a high-rise city
title_full_unstemmed Identification of microalgae cultured in Bold’s Basal medium from freshwater samples, from a high-rise city
title_short Identification of microalgae cultured in Bold’s Basal medium from freshwater samples, from a high-rise city
title_sort identification of microalgae cultured in bold’s basal medium from freshwater samples, from a high-rise city
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904821/
https://www.ncbi.nlm.nih.gov/pubmed/33627771
http://dx.doi.org/10.1038/s41598-021-84112-0
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