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The secreted endoribonuclease ENDU-2 from the soma protects germline immortality in C. elegans
Multicellular organisms coordinate tissue specific responses to environmental information via both cell-autonomous and non-autonomous mechanisms. In addition to secreted ligands, recent reports implicated release of small RNAs in regulating gene expression across tissue boundaries. Here, we show tha...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904951/ https://www.ncbi.nlm.nih.gov/pubmed/33627668 http://dx.doi.org/10.1038/s41467-021-21516-6 |
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author | Qi, Wenjing Gromoff, Erika D. V. Xu, Fan Zhao, Qian Yang, Wei Pfeifer, Dietmar Maier, Wolfgang Long, Lijiang Baumeister, Ralf |
author_facet | Qi, Wenjing Gromoff, Erika D. V. Xu, Fan Zhao, Qian Yang, Wei Pfeifer, Dietmar Maier, Wolfgang Long, Lijiang Baumeister, Ralf |
author_sort | Qi, Wenjing |
collection | PubMed |
description | Multicellular organisms coordinate tissue specific responses to environmental information via both cell-autonomous and non-autonomous mechanisms. In addition to secreted ligands, recent reports implicated release of small RNAs in regulating gene expression across tissue boundaries. Here, we show that the conserved poly-U specific endoribonuclease ENDU-2 in C. elegans is secreted from the soma and taken-up by the germline to ensure germline immortality at elevated temperature. ENDU-2 binds to mature mRNAs and negatively regulates mRNA abundance both in the soma and the germline. While ENDU-2 promotes RNA decay in the soma directly via its endoribonuclease activity, ENDU-2 prevents misexpression of soma-specific genes in the germline and preserves germline immortality independent of its RNA-cleavage activity. In summary, our results suggest that the secreted RNase ENDU-2 regulates gene expression across tissue boundaries in response to temperature alterations and contributes to maintenance of stem cell immortality, probably via retaining a stem cell specific program of gene expression. |
format | Online Article Text |
id | pubmed-7904951 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-79049512021-03-11 The secreted endoribonuclease ENDU-2 from the soma protects germline immortality in C. elegans Qi, Wenjing Gromoff, Erika D. V. Xu, Fan Zhao, Qian Yang, Wei Pfeifer, Dietmar Maier, Wolfgang Long, Lijiang Baumeister, Ralf Nat Commun Article Multicellular organisms coordinate tissue specific responses to environmental information via both cell-autonomous and non-autonomous mechanisms. In addition to secreted ligands, recent reports implicated release of small RNAs in regulating gene expression across tissue boundaries. Here, we show that the conserved poly-U specific endoribonuclease ENDU-2 in C. elegans is secreted from the soma and taken-up by the germline to ensure germline immortality at elevated temperature. ENDU-2 binds to mature mRNAs and negatively regulates mRNA abundance both in the soma and the germline. While ENDU-2 promotes RNA decay in the soma directly via its endoribonuclease activity, ENDU-2 prevents misexpression of soma-specific genes in the germline and preserves germline immortality independent of its RNA-cleavage activity. In summary, our results suggest that the secreted RNase ENDU-2 regulates gene expression across tissue boundaries in response to temperature alterations and contributes to maintenance of stem cell immortality, probably via retaining a stem cell specific program of gene expression. Nature Publishing Group UK 2021-02-24 /pmc/articles/PMC7904951/ /pubmed/33627668 http://dx.doi.org/10.1038/s41467-021-21516-6 Text en © The Author(s) 2021, corrected publication 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Qi, Wenjing Gromoff, Erika D. V. Xu, Fan Zhao, Qian Yang, Wei Pfeifer, Dietmar Maier, Wolfgang Long, Lijiang Baumeister, Ralf The secreted endoribonuclease ENDU-2 from the soma protects germline immortality in C. elegans |
title | The secreted endoribonuclease ENDU-2 from the soma protects germline immortality in C. elegans |
title_full | The secreted endoribonuclease ENDU-2 from the soma protects germline immortality in C. elegans |
title_fullStr | The secreted endoribonuclease ENDU-2 from the soma protects germline immortality in C. elegans |
title_full_unstemmed | The secreted endoribonuclease ENDU-2 from the soma protects germline immortality in C. elegans |
title_short | The secreted endoribonuclease ENDU-2 from the soma protects germline immortality in C. elegans |
title_sort | secreted endoribonuclease endu-2 from the soma protects germline immortality in c. elegans |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904951/ https://www.ncbi.nlm.nih.gov/pubmed/33627668 http://dx.doi.org/10.1038/s41467-021-21516-6 |
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