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Macromolecular crowding effects on the kinetics of opposing reactions catalyzed by alcohol dehydrogenase
In order to better understand how the complex, densely packed, heterogeneous milieu of a cell influences enzyme kinetics, we exposed opposing reactions catalyzed by yeast alcohol dehydrogenase (YADH) to both synthetic and protein crowders ranging from 10 to 550 kDa. The results reveal that the effec...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7905371/ https://www.ncbi.nlm.nih.gov/pubmed/33665382 http://dx.doi.org/10.1016/j.bbrep.2021.100956 |