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Establishment of in vitro 3D spheroid cell cultivation from human gynecologic cancer tissues

Advanced-stage gynecologic cancer remains a life-threatening disease. Here, we present a protocol for establishment of stable in vitro 3D spheroid cells derived from human uterine endometrial and ovarian cancer tissues. The tumor-derived spheroid cells have cancer stem cell-related characteristics,...

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Detalles Bibliográficos
Autores principales: Ueda, Haruka, Mori, Yutaro, Yamawaki, Kaoru, Ishiguro, Tatsuya, Ohata, Hirokazu, Sato, Ai, Sugino, Kentaro, Yachida, Nozomi, Yamaguchi, Manako, Suda, Kazuaki, Tamura, Ryo, Yoshihara, Kosuke, Okamoto, Koji, Enomoto, Takayuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7905465/
https://www.ncbi.nlm.nih.gov/pubmed/33665634
http://dx.doi.org/10.1016/j.xpro.2021.100354

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