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Establishment of in vitro 3D spheroid cell cultivation from human gynecologic cancer tissues
Advanced-stage gynecologic cancer remains a life-threatening disease. Here, we present a protocol for establishment of stable in vitro 3D spheroid cells derived from human uterine endometrial and ovarian cancer tissues. The tumor-derived spheroid cells have cancer stem cell-related characteristics,...
Autores principales: | Ueda, Haruka, Mori, Yutaro, Yamawaki, Kaoru, Ishiguro, Tatsuya, Ohata, Hirokazu, Sato, Ai, Sugino, Kentaro, Yachida, Nozomi, Yamaguchi, Manako, Suda, Kazuaki, Tamura, Ryo, Yoshihara, Kosuke, Okamoto, Koji, Enomoto, Takayuki |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7905465/ https://www.ncbi.nlm.nih.gov/pubmed/33665634 http://dx.doi.org/10.1016/j.xpro.2021.100354 |
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