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Quercetin attenuates the reduction of parvalbumin in middle cerebral artery occlusion animal model
BACKGROUND: Calcium is a critical factor involved in modulation of essential cellular functions. Parvalbumin is a calcium buffering protein that regulates intracellular calcium concentrations. It prevents rises in calcium concentrations and inhibits apoptotic processes during ischemic injury. Querce...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7905853/ https://www.ncbi.nlm.nih.gov/pubmed/33632310 http://dx.doi.org/10.1186/s42826-021-00086-0 |
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author | Park, Dong-Ju Kang, Ju-Bin Shah, Fawad-Ali Koh, Phil-Ok |
author_facet | Park, Dong-Ju Kang, Ju-Bin Shah, Fawad-Ali Koh, Phil-Ok |
author_sort | Park, Dong-Ju |
collection | PubMed |
description | BACKGROUND: Calcium is a critical factor involved in modulation of essential cellular functions. Parvalbumin is a calcium buffering protein that regulates intracellular calcium concentrations. It prevents rises in calcium concentrations and inhibits apoptotic processes during ischemic injury. Quercetin exerts potent antioxidant and anti-apoptotic effects during brain ischemia. We investigated whether quercetin can regulate parvalbumin expression in cerebral ischemia and glutamate toxicity-induced neuronal cell death. Adult male rats were treated with vehicle or quercetin (10 mg/kg) 30 min prior to middle cerebral artery occlusion (MCAO) and cerebral cortical tissues were collected 24 h after MCAO. We used various techniques including Western blot, reverse transcription-PCR, and immunohistochemical staining to elucidate the changes of parvalbumin expression. RESULTS: Quercetin ameliorated MCAO-induced neurological deficits and behavioral changes. Moreover, quercetin prevented MCAO-induced a decrease in parvalbumin expression. CONCLUSIONS: These findings suggest that quercetin exerts a neuroprotective effect through regulation of parvalbumin expression. |
format | Online Article Text |
id | pubmed-7905853 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-79058532021-02-25 Quercetin attenuates the reduction of parvalbumin in middle cerebral artery occlusion animal model Park, Dong-Ju Kang, Ju-Bin Shah, Fawad-Ali Koh, Phil-Ok Lab Anim Res Research BACKGROUND: Calcium is a critical factor involved in modulation of essential cellular functions. Parvalbumin is a calcium buffering protein that regulates intracellular calcium concentrations. It prevents rises in calcium concentrations and inhibits apoptotic processes during ischemic injury. Quercetin exerts potent antioxidant and anti-apoptotic effects during brain ischemia. We investigated whether quercetin can regulate parvalbumin expression in cerebral ischemia and glutamate toxicity-induced neuronal cell death. Adult male rats were treated with vehicle or quercetin (10 mg/kg) 30 min prior to middle cerebral artery occlusion (MCAO) and cerebral cortical tissues were collected 24 h after MCAO. We used various techniques including Western blot, reverse transcription-PCR, and immunohistochemical staining to elucidate the changes of parvalbumin expression. RESULTS: Quercetin ameliorated MCAO-induced neurological deficits and behavioral changes. Moreover, quercetin prevented MCAO-induced a decrease in parvalbumin expression. CONCLUSIONS: These findings suggest that quercetin exerts a neuroprotective effect through regulation of parvalbumin expression. BioMed Central 2021-02-25 /pmc/articles/PMC7905853/ /pubmed/33632310 http://dx.doi.org/10.1186/s42826-021-00086-0 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Park, Dong-Ju Kang, Ju-Bin Shah, Fawad-Ali Koh, Phil-Ok Quercetin attenuates the reduction of parvalbumin in middle cerebral artery occlusion animal model |
title | Quercetin attenuates the reduction of parvalbumin in middle cerebral artery occlusion animal model |
title_full | Quercetin attenuates the reduction of parvalbumin in middle cerebral artery occlusion animal model |
title_fullStr | Quercetin attenuates the reduction of parvalbumin in middle cerebral artery occlusion animal model |
title_full_unstemmed | Quercetin attenuates the reduction of parvalbumin in middle cerebral artery occlusion animal model |
title_short | Quercetin attenuates the reduction of parvalbumin in middle cerebral artery occlusion animal model |
title_sort | quercetin attenuates the reduction of parvalbumin in middle cerebral artery occlusion animal model |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7905853/ https://www.ncbi.nlm.nih.gov/pubmed/33632310 http://dx.doi.org/10.1186/s42826-021-00086-0 |
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