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Ionic Liquid-Induced Phase-Separated Domains in Lipid Multilayers Probed by X-ray Scattering Studies
[Image: see text] A cellular membrane, primarily a lipid bilayer, surrounds the internal components of a biological cell from the external components. This self-assembled bilayer is known to be perturbed by ionic liquids (ILs) causing malfunctioning of a cellular organism. In the present study, surf...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7905935/ https://www.ncbi.nlm.nih.gov/pubmed/33644605 http://dx.doi.org/10.1021/acsomega.0c06014 |
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author | Gupta, Ritika Singh, Arnab Srihari, Velaga Ghosh, Sajal K. |
author_facet | Gupta, Ritika Singh, Arnab Srihari, Velaga Ghosh, Sajal K. |
author_sort | Gupta, Ritika |
collection | PubMed |
description | [Image: see text] A cellular membrane, primarily a lipid bilayer, surrounds the internal components of a biological cell from the external components. This self-assembled bilayer is known to be perturbed by ionic liquids (ILs) causing malfunctioning of a cellular organism. In the present study, surface-sensitive X-ray scattering techniques have been employed to understand this structural perturbation in a lipid multilayer system formed by a zwitterionic phospholipid, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine. The ammonium and phosphonium-based ILs with methanesulfonate anions are observed to induce phase-separated domains in the plane of a bilayer. The lamellar X-ray diffraction peaks suggest these domains to correlate across the bilayers in a smectic liquid crystalline phase. This induced IL-rich lamellar phase has a very low lamellar repeat distance, suggesting the formation of an interdigitated bilayer. The IL-poor phase closely related to the pristine lipid phase shows a decrement in the in-plane chain lattice parameters with a reduced tilt angle. The ammonium and phosphonium-based ILs with a relatively bulky anion, p-toluenemethanesulfonate, have shown a similar effect. |
format | Online Article Text |
id | pubmed-7905935 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-79059352021-02-26 Ionic Liquid-Induced Phase-Separated Domains in Lipid Multilayers Probed by X-ray Scattering Studies Gupta, Ritika Singh, Arnab Srihari, Velaga Ghosh, Sajal K. ACS Omega [Image: see text] A cellular membrane, primarily a lipid bilayer, surrounds the internal components of a biological cell from the external components. This self-assembled bilayer is known to be perturbed by ionic liquids (ILs) causing malfunctioning of a cellular organism. In the present study, surface-sensitive X-ray scattering techniques have been employed to understand this structural perturbation in a lipid multilayer system formed by a zwitterionic phospholipid, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine. The ammonium and phosphonium-based ILs with methanesulfonate anions are observed to induce phase-separated domains in the plane of a bilayer. The lamellar X-ray diffraction peaks suggest these domains to correlate across the bilayers in a smectic liquid crystalline phase. This induced IL-rich lamellar phase has a very low lamellar repeat distance, suggesting the formation of an interdigitated bilayer. The IL-poor phase closely related to the pristine lipid phase shows a decrement in the in-plane chain lattice parameters with a reduced tilt angle. The ammonium and phosphonium-based ILs with a relatively bulky anion, p-toluenemethanesulfonate, have shown a similar effect. American Chemical Society 2021-02-05 /pmc/articles/PMC7905935/ /pubmed/33644605 http://dx.doi.org/10.1021/acsomega.0c06014 Text en © 2021 The Authors. Published by American Chemical Society Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Gupta, Ritika Singh, Arnab Srihari, Velaga Ghosh, Sajal K. Ionic Liquid-Induced Phase-Separated Domains in Lipid Multilayers Probed by X-ray Scattering Studies |
title | Ionic Liquid-Induced Phase-Separated Domains in Lipid
Multilayers Probed by X-ray Scattering Studies |
title_full | Ionic Liquid-Induced Phase-Separated Domains in Lipid
Multilayers Probed by X-ray Scattering Studies |
title_fullStr | Ionic Liquid-Induced Phase-Separated Domains in Lipid
Multilayers Probed by X-ray Scattering Studies |
title_full_unstemmed | Ionic Liquid-Induced Phase-Separated Domains in Lipid
Multilayers Probed by X-ray Scattering Studies |
title_short | Ionic Liquid-Induced Phase-Separated Domains in Lipid
Multilayers Probed by X-ray Scattering Studies |
title_sort | ionic liquid-induced phase-separated domains in lipid
multilayers probed by x-ray scattering studies |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7905935/ https://www.ncbi.nlm.nih.gov/pubmed/33644605 http://dx.doi.org/10.1021/acsomega.0c06014 |
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