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Key microRNAs and hub genes associated with poor prognosis in lung adenocarcinoma

In the study, we obtained 36 pairs of lung adenocarcinoma (LUAD) tissues and adjacent non-tumorous tissues. Then, we chose a specific hub-target gene of miRNA and used qRT-PCR to evaluate the expression of PECAM1. We found that the expression level of PECAM1 mRNA in LUAD was significantly lower than...

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Autores principales: Ye, Guan-Chao, Liu, Ya-Fei, Huang, Lan, Zhang, Chun-Yang, Sheng, Yin-Liang, Wu, Bin, Han, Lu, Wu, Chun-Li, Dong, Bo, Qi, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7906143/
https://www.ncbi.nlm.nih.gov/pubmed/33461176
http://dx.doi.org/10.18632/aging.202337
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author Ye, Guan-Chao
Liu, Ya-Fei
Huang, Lan
Zhang, Chun-Yang
Sheng, Yin-Liang
Wu, Bin
Han, Lu
Wu, Chun-Li
Dong, Bo
Qi, Yu
author_facet Ye, Guan-Chao
Liu, Ya-Fei
Huang, Lan
Zhang, Chun-Yang
Sheng, Yin-Liang
Wu, Bin
Han, Lu
Wu, Chun-Li
Dong, Bo
Qi, Yu
author_sort Ye, Guan-Chao
collection PubMed
description In the study, we obtained 36 pairs of lung adenocarcinoma (LUAD) tissues and adjacent non-tumorous tissues. Then, we chose a specific hub-target gene of miRNA and used qRT-PCR to evaluate the expression of PECAM1. We found that the expression level of PECAM1 mRNA in LUAD was significantly lower than that in adjacent nontumor tissues (P<0.0001). Univariate and multivariate analyses were conducted on 481 LUAD patients from The Cancer Genome Atlas (TCGA) according to the Cox proportional hazard regression model to evaluate the impact of PECAM1 expression and other clinicopathological factors on survival. The results showed that the low expression of PECAM1 was an important independent predictor of poor overall survival (HR, 0.704; 95% CI, 0.518-0.957; P = 0.025). Based on the Tumor Immune Estimation Resource (TIMER) database, the relationship between PECAM1 expression and B cell, CD8+ T cell, CD4+ T cell, macrophage, neutrophil, and dendritic cell infiltration was weak in LUAD (P<0.01). In particular, a more significant positive correlation between PECAM1 expression and HLA-complex members, CD1C, NRP1, and ITGAX expression in dendritic cell was detected in LUAD. The mechanism which PECAM1 involved in the development of LUAD may be closely related to changes in the immune microenvironment.
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spelling pubmed-79061432021-03-04 Key microRNAs and hub genes associated with poor prognosis in lung adenocarcinoma Ye, Guan-Chao Liu, Ya-Fei Huang, Lan Zhang, Chun-Yang Sheng, Yin-Liang Wu, Bin Han, Lu Wu, Chun-Li Dong, Bo Qi, Yu Aging (Albany NY) Research Paper In the study, we obtained 36 pairs of lung adenocarcinoma (LUAD) tissues and adjacent non-tumorous tissues. Then, we chose a specific hub-target gene of miRNA and used qRT-PCR to evaluate the expression of PECAM1. We found that the expression level of PECAM1 mRNA in LUAD was significantly lower than that in adjacent nontumor tissues (P<0.0001). Univariate and multivariate analyses were conducted on 481 LUAD patients from The Cancer Genome Atlas (TCGA) according to the Cox proportional hazard regression model to evaluate the impact of PECAM1 expression and other clinicopathological factors on survival. The results showed that the low expression of PECAM1 was an important independent predictor of poor overall survival (HR, 0.704; 95% CI, 0.518-0.957; P = 0.025). Based on the Tumor Immune Estimation Resource (TIMER) database, the relationship between PECAM1 expression and B cell, CD8+ T cell, CD4+ T cell, macrophage, neutrophil, and dendritic cell infiltration was weak in LUAD (P<0.01). In particular, a more significant positive correlation between PECAM1 expression and HLA-complex members, CD1C, NRP1, and ITGAX expression in dendritic cell was detected in LUAD. The mechanism which PECAM1 involved in the development of LUAD may be closely related to changes in the immune microenvironment. Impact Journals 2021-01-10 /pmc/articles/PMC7906143/ /pubmed/33461176 http://dx.doi.org/10.18632/aging.202337 Text en Copyright: © 2021 Ye et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Ye, Guan-Chao
Liu, Ya-Fei
Huang, Lan
Zhang, Chun-Yang
Sheng, Yin-Liang
Wu, Bin
Han, Lu
Wu, Chun-Li
Dong, Bo
Qi, Yu
Key microRNAs and hub genes associated with poor prognosis in lung adenocarcinoma
title Key microRNAs and hub genes associated with poor prognosis in lung adenocarcinoma
title_full Key microRNAs and hub genes associated with poor prognosis in lung adenocarcinoma
title_fullStr Key microRNAs and hub genes associated with poor prognosis in lung adenocarcinoma
title_full_unstemmed Key microRNAs and hub genes associated with poor prognosis in lung adenocarcinoma
title_short Key microRNAs and hub genes associated with poor prognosis in lung adenocarcinoma
title_sort key micrornas and hub genes associated with poor prognosis in lung adenocarcinoma
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7906143/
https://www.ncbi.nlm.nih.gov/pubmed/33461176
http://dx.doi.org/10.18632/aging.202337
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