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Optical multi-channel interrogation instrument for bacterial colony characterization

A single instrument that includes multiple optical channels was developed to simultaneously measure various optical and associated biophysical characteristics of a bacterial colony. The multi-channel device can provide five distinct optical features without the need to transfer the sample to multipl...

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Detalles Bibliográficos
Autores principales: Doh, Iyll-Joon, Kim, Huisung, Sturgis, Jennifer, Rajwa, Bartek, Robinson, J. Paul, Bae, Euiwon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7906345/
https://www.ncbi.nlm.nih.gov/pubmed/33630969
http://dx.doi.org/10.1371/journal.pone.0247721
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author Doh, Iyll-Joon
Kim, Huisung
Sturgis, Jennifer
Rajwa, Bartek
Robinson, J. Paul
Bae, Euiwon
author_facet Doh, Iyll-Joon
Kim, Huisung
Sturgis, Jennifer
Rajwa, Bartek
Robinson, J. Paul
Bae, Euiwon
author_sort Doh, Iyll-Joon
collection PubMed
description A single instrument that includes multiple optical channels was developed to simultaneously measure various optical and associated biophysical characteristics of a bacterial colony. The multi-channel device can provide five distinct optical features without the need to transfer the sample to multiple locations or instruments. The available measurement channels are bright-field light microscopy, 3-D colony-morphology map, 2-D spatial optical-density distribution, spectral forward-scattering pattern, and spectral optical density. The series of multiple morphological interrogations is beneficial in understanding the bio-optical features of a bacterial colony and the correlations among them, resulting in an enhanced power of phenotypic bacterial discrimination. To enable a one-shot interrogation, a confocal laser scanning module was built as an add-on to an upright microscope. Three different-wavelength diode lasers were used for the spectral analysis, and high-speed pin photodiodes and CMOS sensors were utilized as detectors to measure the spectral OD and light-scatter pattern. The proposed instrument and algorithms were evaluated with four bacterial genera, Escherichia coli, Listeria innocua, Salmonella Typhimurium, and Staphylococcus aureus; their resulting data provided a more complete picture of the optical characterization of bacterial colonies.
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spelling pubmed-79063452021-03-03 Optical multi-channel interrogation instrument for bacterial colony characterization Doh, Iyll-Joon Kim, Huisung Sturgis, Jennifer Rajwa, Bartek Robinson, J. Paul Bae, Euiwon PLoS One Research Article A single instrument that includes multiple optical channels was developed to simultaneously measure various optical and associated biophysical characteristics of a bacterial colony. The multi-channel device can provide five distinct optical features without the need to transfer the sample to multiple locations or instruments. The available measurement channels are bright-field light microscopy, 3-D colony-morphology map, 2-D spatial optical-density distribution, spectral forward-scattering pattern, and spectral optical density. The series of multiple morphological interrogations is beneficial in understanding the bio-optical features of a bacterial colony and the correlations among them, resulting in an enhanced power of phenotypic bacterial discrimination. To enable a one-shot interrogation, a confocal laser scanning module was built as an add-on to an upright microscope. Three different-wavelength diode lasers were used for the spectral analysis, and high-speed pin photodiodes and CMOS sensors were utilized as detectors to measure the spectral OD and light-scatter pattern. The proposed instrument and algorithms were evaluated with four bacterial genera, Escherichia coli, Listeria innocua, Salmonella Typhimurium, and Staphylococcus aureus; their resulting data provided a more complete picture of the optical characterization of bacterial colonies. Public Library of Science 2021-02-25 /pmc/articles/PMC7906345/ /pubmed/33630969 http://dx.doi.org/10.1371/journal.pone.0247721 Text en © 2021 Doh et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Doh, Iyll-Joon
Kim, Huisung
Sturgis, Jennifer
Rajwa, Bartek
Robinson, J. Paul
Bae, Euiwon
Optical multi-channel interrogation instrument for bacterial colony characterization
title Optical multi-channel interrogation instrument for bacterial colony characterization
title_full Optical multi-channel interrogation instrument for bacterial colony characterization
title_fullStr Optical multi-channel interrogation instrument for bacterial colony characterization
title_full_unstemmed Optical multi-channel interrogation instrument for bacterial colony characterization
title_short Optical multi-channel interrogation instrument for bacterial colony characterization
title_sort optical multi-channel interrogation instrument for bacterial colony characterization
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7906345/
https://www.ncbi.nlm.nih.gov/pubmed/33630969
http://dx.doi.org/10.1371/journal.pone.0247721
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