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Biomineralization of three calcium silicate-based cements after implantation in rat subcutaneous tissue

OBJECTIVES: The aim of this study was to evaluate the dystrophic mineralization deposits from 3 calcium silicate-based cements (Micro-Mega mineral trioxide aggregate [MM-MTA], Biodentine [BD], and EndoSequence Root Repair Material [ESRRM] putty) over time after subcutaneous implantation into rats. M...

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Autores principales: Talabani, Ranjdar Mahmood, Garib, Balkees Taha, Masaeli, Reza, Zandsalimi, Kavosh, Ketabat, Farinaz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Academy of Conservative Dentistry 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7906840/
https://www.ncbi.nlm.nih.gov/pubmed/33680890
http://dx.doi.org/10.5395/rde.2021.46.e1
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author Talabani, Ranjdar Mahmood
Garib, Balkees Taha
Masaeli, Reza
Zandsalimi, Kavosh
Ketabat, Farinaz
author_facet Talabani, Ranjdar Mahmood
Garib, Balkees Taha
Masaeli, Reza
Zandsalimi, Kavosh
Ketabat, Farinaz
author_sort Talabani, Ranjdar Mahmood
collection PubMed
description OBJECTIVES: The aim of this study was to evaluate the dystrophic mineralization deposits from 3 calcium silicate-based cements (Micro-Mega mineral trioxide aggregate [MM-MTA], Biodentine [BD], and EndoSequence Root Repair Material [ESRRM] putty) over time after subcutaneous implantation into rats. MATERIALS AND METHODS: Forty-five silicon tubes containing the tested materials and 15 empty tubes (serving as a control group) were subcutaneously implanted into the backs of 15 Wistar rats. At 1, 4, and 8 weeks after implantation, the animals were euthanized (n = 5 animals/group), and the silicon tubes were removed with the surrounding tissues. Histopathological tissue sections were stained with von Kossa stain to assess mineralization. Scanning electron microscopy and energy-dispersive X-ray spectroscopy (SEM/EDX) were also used to assess the chemical components of the surface precipitates deposited on the implant and the pattern of calcium and phosphorus distribution at the material-tissue interface. The calcium-to-phosphorus ratios were compared using the non-parametric Kruskal-Wallis test at a significance level of 5%. RESULTS: The von Kossa staining showed that both BD and ESRRM putty induced mineralization starting at week 1; this mineralization increased further until the end of the study. In contrast, MM-MTA induced dystrophic calcification later, from 4 weeks onward. SEM/EDX showed no statistically significant differences in the calcium- and phosphorus-rich areas among the 3 materials at any time point (p > 0.05). CONCLUSIONS: After subcutaneous implantation, biomineralization of the 3-calcium silicate-based cements started early and increased over time, and all 3 tested cements generated calcium- and phosphorus-containing surface precipitates.
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spelling pubmed-79068402021-03-04 Biomineralization of three calcium silicate-based cements after implantation in rat subcutaneous tissue Talabani, Ranjdar Mahmood Garib, Balkees Taha Masaeli, Reza Zandsalimi, Kavosh Ketabat, Farinaz Restor Dent Endod Research Article OBJECTIVES: The aim of this study was to evaluate the dystrophic mineralization deposits from 3 calcium silicate-based cements (Micro-Mega mineral trioxide aggregate [MM-MTA], Biodentine [BD], and EndoSequence Root Repair Material [ESRRM] putty) over time after subcutaneous implantation into rats. MATERIALS AND METHODS: Forty-five silicon tubes containing the tested materials and 15 empty tubes (serving as a control group) were subcutaneously implanted into the backs of 15 Wistar rats. At 1, 4, and 8 weeks after implantation, the animals were euthanized (n = 5 animals/group), and the silicon tubes were removed with the surrounding tissues. Histopathological tissue sections were stained with von Kossa stain to assess mineralization. Scanning electron microscopy and energy-dispersive X-ray spectroscopy (SEM/EDX) were also used to assess the chemical components of the surface precipitates deposited on the implant and the pattern of calcium and phosphorus distribution at the material-tissue interface. The calcium-to-phosphorus ratios were compared using the non-parametric Kruskal-Wallis test at a significance level of 5%. RESULTS: The von Kossa staining showed that both BD and ESRRM putty induced mineralization starting at week 1; this mineralization increased further until the end of the study. In contrast, MM-MTA induced dystrophic calcification later, from 4 weeks onward. SEM/EDX showed no statistically significant differences in the calcium- and phosphorus-rich areas among the 3 materials at any time point (p > 0.05). CONCLUSIONS: After subcutaneous implantation, biomineralization of the 3-calcium silicate-based cements started early and increased over time, and all 3 tested cements generated calcium- and phosphorus-containing surface precipitates. The Korean Academy of Conservative Dentistry 2020-12-02 /pmc/articles/PMC7906840/ /pubmed/33680890 http://dx.doi.org/10.5395/rde.2021.46.e1 Text en Copyright © 2021. The Korean Academy of Conservative Dentistry https://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Talabani, Ranjdar Mahmood
Garib, Balkees Taha
Masaeli, Reza
Zandsalimi, Kavosh
Ketabat, Farinaz
Biomineralization of three calcium silicate-based cements after implantation in rat subcutaneous tissue
title Biomineralization of three calcium silicate-based cements after implantation in rat subcutaneous tissue
title_full Biomineralization of three calcium silicate-based cements after implantation in rat subcutaneous tissue
title_fullStr Biomineralization of three calcium silicate-based cements after implantation in rat subcutaneous tissue
title_full_unstemmed Biomineralization of three calcium silicate-based cements after implantation in rat subcutaneous tissue
title_short Biomineralization of three calcium silicate-based cements after implantation in rat subcutaneous tissue
title_sort biomineralization of three calcium silicate-based cements after implantation in rat subcutaneous tissue
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7906840/
https://www.ncbi.nlm.nih.gov/pubmed/33680890
http://dx.doi.org/10.5395/rde.2021.46.e1
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