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The effect of deformation of absorbing scatterers on Mie-type signatures in infrared microspectroscopy
Mie-type scattering features such as ripples (i.e., sharp shape-resonance peaks) and wiggles (i.e., broad oscillations), are frequently-observed scattering phenomena in infrared microspectroscopy of cells and tissues. They appear in general when the wavelength of electromagnetic radiation is of the...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7907113/ https://www.ncbi.nlm.nih.gov/pubmed/33633244 http://dx.doi.org/10.1038/s41598-021-84064-5 |
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author | Brandsrud, Maren Anna Blümel, Reinhold Solheim, Johanne Heitmann Kohler, Achim |
author_facet | Brandsrud, Maren Anna Blümel, Reinhold Solheim, Johanne Heitmann Kohler, Achim |
author_sort | Brandsrud, Maren Anna |
collection | PubMed |
description | Mie-type scattering features such as ripples (i.e., sharp shape-resonance peaks) and wiggles (i.e., broad oscillations), are frequently-observed scattering phenomena in infrared microspectroscopy of cells and tissues. They appear in general when the wavelength of electromagnetic radiation is of the same order as the size of the scatterer. By use of approximations to the Mie solutions for spheres, iterative algorithms have been developed to retrieve pure absorbance spectra. However, the question remains to what extent the Mie solutions, and approximations thereof, describe the extinction efficiency in practical situations where the shapes of scatterers deviate considerably from spheres. The aim of the current study is to investigate how deviations from a spherical scatterer can change the extinction properties of the scatterer in the context of chaos in wave systems. For this purpose, we investigate a chaotic scatterer and compare it with an elliptically shaped scatterer, which exhibits only regular scattering. We find that chaotic scattering has an accelerating effect on the disappearance of Mie ripples. We further show that the presence of absorption and the high numerical aperture of infrared microscopes does not explain the absence of ripples in most measurements of biological samples. |
format | Online Article Text |
id | pubmed-7907113 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-79071132021-02-26 The effect of deformation of absorbing scatterers on Mie-type signatures in infrared microspectroscopy Brandsrud, Maren Anna Blümel, Reinhold Solheim, Johanne Heitmann Kohler, Achim Sci Rep Article Mie-type scattering features such as ripples (i.e., sharp shape-resonance peaks) and wiggles (i.e., broad oscillations), are frequently-observed scattering phenomena in infrared microspectroscopy of cells and tissues. They appear in general when the wavelength of electromagnetic radiation is of the same order as the size of the scatterer. By use of approximations to the Mie solutions for spheres, iterative algorithms have been developed to retrieve pure absorbance spectra. However, the question remains to what extent the Mie solutions, and approximations thereof, describe the extinction efficiency in practical situations where the shapes of scatterers deviate considerably from spheres. The aim of the current study is to investigate how deviations from a spherical scatterer can change the extinction properties of the scatterer in the context of chaos in wave systems. For this purpose, we investigate a chaotic scatterer and compare it with an elliptically shaped scatterer, which exhibits only regular scattering. We find that chaotic scattering has an accelerating effect on the disappearance of Mie ripples. We further show that the presence of absorption and the high numerical aperture of infrared microscopes does not explain the absence of ripples in most measurements of biological samples. Nature Publishing Group UK 2021-02-25 /pmc/articles/PMC7907113/ /pubmed/33633244 http://dx.doi.org/10.1038/s41598-021-84064-5 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Brandsrud, Maren Anna Blümel, Reinhold Solheim, Johanne Heitmann Kohler, Achim The effect of deformation of absorbing scatterers on Mie-type signatures in infrared microspectroscopy |
title | The effect of deformation of absorbing scatterers on Mie-type signatures in infrared microspectroscopy |
title_full | The effect of deformation of absorbing scatterers on Mie-type signatures in infrared microspectroscopy |
title_fullStr | The effect of deformation of absorbing scatterers on Mie-type signatures in infrared microspectroscopy |
title_full_unstemmed | The effect of deformation of absorbing scatterers on Mie-type signatures in infrared microspectroscopy |
title_short | The effect of deformation of absorbing scatterers on Mie-type signatures in infrared microspectroscopy |
title_sort | effect of deformation of absorbing scatterers on mie-type signatures in infrared microspectroscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7907113/ https://www.ncbi.nlm.nih.gov/pubmed/33633244 http://dx.doi.org/10.1038/s41598-021-84064-5 |
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