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Insecticide resistance and underlying targets-site and metabolic mechanisms in Aedes aegypti and Aedes albopictus from Lahore, Pakistan

Insecticide resistant Aedes populations have recently been reported in Pakistan, imposing a threat to their control. We aimed to evaluate the susceptibility of Aedes aegypti and Aedes albopictus populations from Lahore to WHO-recommended insecticides and to investigate metabolic and target-site resi...

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Autores principales: Rahman, Rafi Ur, Souza, Barbara, Uddin, Iftikhar, Carrara, Luana, Brito, Luiz Paulo, Costa, Monique Melo, Mahmood, Muhammad Asif, Khan, Sozaina, Lima, Jose Bento Pereira, Martins, Ademir Jesus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7907206/
https://www.ncbi.nlm.nih.gov/pubmed/33633183
http://dx.doi.org/10.1038/s41598-021-83465-w
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author Rahman, Rafi Ur
Souza, Barbara
Uddin, Iftikhar
Carrara, Luana
Brito, Luiz Paulo
Costa, Monique Melo
Mahmood, Muhammad Asif
Khan, Sozaina
Lima, Jose Bento Pereira
Martins, Ademir Jesus
author_facet Rahman, Rafi Ur
Souza, Barbara
Uddin, Iftikhar
Carrara, Luana
Brito, Luiz Paulo
Costa, Monique Melo
Mahmood, Muhammad Asif
Khan, Sozaina
Lima, Jose Bento Pereira
Martins, Ademir Jesus
author_sort Rahman, Rafi Ur
collection PubMed
description Insecticide resistant Aedes populations have recently been reported in Pakistan, imposing a threat to their control. We aimed to evaluate the susceptibility of Aedes aegypti and Aedes albopictus populations from Lahore to WHO-recommended insecticides and to investigate metabolic and target-site resistance mechanisms. For this purpose, we first carried out bioassays with the larvicides temephos and pyriproxyfen, and the adulticides malathion, permethrin, deltamethrin, alpha-cypermethrin, and etofenprox. We looked for Knockdown resistance mutations (kdr) by qPCR, High-Resolution Melt (HRM), and sequencing. In order to explore the role of detoxifying enzymes in resistance, we carried out synergist bioassay with both species and then checked the expression of CYP9M6, CYP9J10, CYP9J28, CYP6BB2, CCAe3a, and SAP2 genes in Ae. aegypti. Both species were susceptible to organophosphates and the insect growth regulator, however resistant to all pyrethroids. We are reporting the kdr haplotypes 1520Ile + 1534Cys and T1520 + 1534Cys in high frequencies in Ae. aegypti while Ae. albopictus only exhibited the alteration L882M. PBO increased the sensitivity to permethrin in Ae. aegypti, suggesting the participation of P450 genes in conferring resistance, and indeed, CYP928 was highly expressed. We presume that dengue vectors in Lahore city are resistant to pyrethroids, probably due to multiple mechanisms, such as kdr mutations and P450 overexpression.
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spelling pubmed-79072062021-02-26 Insecticide resistance and underlying targets-site and metabolic mechanisms in Aedes aegypti and Aedes albopictus from Lahore, Pakistan Rahman, Rafi Ur Souza, Barbara Uddin, Iftikhar Carrara, Luana Brito, Luiz Paulo Costa, Monique Melo Mahmood, Muhammad Asif Khan, Sozaina Lima, Jose Bento Pereira Martins, Ademir Jesus Sci Rep Article Insecticide resistant Aedes populations have recently been reported in Pakistan, imposing a threat to their control. We aimed to evaluate the susceptibility of Aedes aegypti and Aedes albopictus populations from Lahore to WHO-recommended insecticides and to investigate metabolic and target-site resistance mechanisms. For this purpose, we first carried out bioassays with the larvicides temephos and pyriproxyfen, and the adulticides malathion, permethrin, deltamethrin, alpha-cypermethrin, and etofenprox. We looked for Knockdown resistance mutations (kdr) by qPCR, High-Resolution Melt (HRM), and sequencing. In order to explore the role of detoxifying enzymes in resistance, we carried out synergist bioassay with both species and then checked the expression of CYP9M6, CYP9J10, CYP9J28, CYP6BB2, CCAe3a, and SAP2 genes in Ae. aegypti. Both species were susceptible to organophosphates and the insect growth regulator, however resistant to all pyrethroids. We are reporting the kdr haplotypes 1520Ile + 1534Cys and T1520 + 1534Cys in high frequencies in Ae. aegypti while Ae. albopictus only exhibited the alteration L882M. PBO increased the sensitivity to permethrin in Ae. aegypti, suggesting the participation of P450 genes in conferring resistance, and indeed, CYP928 was highly expressed. We presume that dengue vectors in Lahore city are resistant to pyrethroids, probably due to multiple mechanisms, such as kdr mutations and P450 overexpression. Nature Publishing Group UK 2021-02-25 /pmc/articles/PMC7907206/ /pubmed/33633183 http://dx.doi.org/10.1038/s41598-021-83465-w Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Rahman, Rafi Ur
Souza, Barbara
Uddin, Iftikhar
Carrara, Luana
Brito, Luiz Paulo
Costa, Monique Melo
Mahmood, Muhammad Asif
Khan, Sozaina
Lima, Jose Bento Pereira
Martins, Ademir Jesus
Insecticide resistance and underlying targets-site and metabolic mechanisms in Aedes aegypti and Aedes albopictus from Lahore, Pakistan
title Insecticide resistance and underlying targets-site and metabolic mechanisms in Aedes aegypti and Aedes albopictus from Lahore, Pakistan
title_full Insecticide resistance and underlying targets-site and metabolic mechanisms in Aedes aegypti and Aedes albopictus from Lahore, Pakistan
title_fullStr Insecticide resistance and underlying targets-site and metabolic mechanisms in Aedes aegypti and Aedes albopictus from Lahore, Pakistan
title_full_unstemmed Insecticide resistance and underlying targets-site and metabolic mechanisms in Aedes aegypti and Aedes albopictus from Lahore, Pakistan
title_short Insecticide resistance and underlying targets-site and metabolic mechanisms in Aedes aegypti and Aedes albopictus from Lahore, Pakistan
title_sort insecticide resistance and underlying targets-site and metabolic mechanisms in aedes aegypti and aedes albopictus from lahore, pakistan
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7907206/
https://www.ncbi.nlm.nih.gov/pubmed/33633183
http://dx.doi.org/10.1038/s41598-021-83465-w
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