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Mechanism of autonomous synchronization of the circadian KaiABC rhythm
The cyanobacterial circadian clock can be reconstituted by mixing three proteins, KaiA, KaiB, and KaiC, in vitro. In this protein mixture, oscillations of the phosphorylation level of KaiC molecules are synchronized to show the coherent oscillations of the ensemble of many molecules. However, the mo...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7907350/ https://www.ncbi.nlm.nih.gov/pubmed/33633230 http://dx.doi.org/10.1038/s41598-021-84008-z |
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author | Sasai, Masaki |
author_facet | Sasai, Masaki |
author_sort | Sasai, Masaki |
collection | PubMed |
description | The cyanobacterial circadian clock can be reconstituted by mixing three proteins, KaiA, KaiB, and KaiC, in vitro. In this protein mixture, oscillations of the phosphorylation level of KaiC molecules are synchronized to show the coherent oscillations of the ensemble of many molecules. However, the molecular mechanism of this synchronization has not yet been fully elucidated. In this paper, we explain a theoretical model that considers the multifold feedback relations among the structure and reactions of KaiC. The simulated KaiC hexamers show stochastic switch-like transitions at the level of single molecules, which are synchronized in the ensemble through the sequestration of KaiA into the KaiC–KaiB–KaiA complexes. The proposed mechanism quantitatively reproduces the synchronization that was observed by mixing two solutions oscillating in different phases. The model results suggest that biochemical assays with varying concentrations of KaiA or KaiB can be used to test this hypothesis. |
format | Online Article Text |
id | pubmed-7907350 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-79073502021-03-02 Mechanism of autonomous synchronization of the circadian KaiABC rhythm Sasai, Masaki Sci Rep Article The cyanobacterial circadian clock can be reconstituted by mixing three proteins, KaiA, KaiB, and KaiC, in vitro. In this protein mixture, oscillations of the phosphorylation level of KaiC molecules are synchronized to show the coherent oscillations of the ensemble of many molecules. However, the molecular mechanism of this synchronization has not yet been fully elucidated. In this paper, we explain a theoretical model that considers the multifold feedback relations among the structure and reactions of KaiC. The simulated KaiC hexamers show stochastic switch-like transitions at the level of single molecules, which are synchronized in the ensemble through the sequestration of KaiA into the KaiC–KaiB–KaiA complexes. The proposed mechanism quantitatively reproduces the synchronization that was observed by mixing two solutions oscillating in different phases. The model results suggest that biochemical assays with varying concentrations of KaiA or KaiB can be used to test this hypothesis. Nature Publishing Group UK 2021-02-25 /pmc/articles/PMC7907350/ /pubmed/33633230 http://dx.doi.org/10.1038/s41598-021-84008-z Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Sasai, Masaki Mechanism of autonomous synchronization of the circadian KaiABC rhythm |
title | Mechanism of autonomous synchronization of the circadian KaiABC rhythm |
title_full | Mechanism of autonomous synchronization of the circadian KaiABC rhythm |
title_fullStr | Mechanism of autonomous synchronization of the circadian KaiABC rhythm |
title_full_unstemmed | Mechanism of autonomous synchronization of the circadian KaiABC rhythm |
title_short | Mechanism of autonomous synchronization of the circadian KaiABC rhythm |
title_sort | mechanism of autonomous synchronization of the circadian kaiabc rhythm |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7907350/ https://www.ncbi.nlm.nih.gov/pubmed/33633230 http://dx.doi.org/10.1038/s41598-021-84008-z |
work_keys_str_mv | AT sasaimasaki mechanismofautonomoussynchronizationofthecircadiankaiabcrhythm |