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Amazonia Seasons Have an Influence in the Composition of Bacterial Gut Microbiota of Mangrove Oysters (Crassostrea gasar)

The mangrove oysters (Crassostrea gasar) are molluscs native to the Amazonia region and their exploration and farming has increased considerably in recent years. These animals are farmed on beds built in the rivers of the Amazonia estuaries and, therefore, the composition of their microbiome should...

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Autores principales: Conceição, Marcos Vinícius Reis, Costa, Sávio Souza, Schaan, Ana Paula, Ribeiro-dos-Santos, Ândrea Kely Campos, Silva, Artur, das Graças, Diego Assis, Schneider, Maria Paula Cruz, Baraúna, Rafael Azevedo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7907636/
https://www.ncbi.nlm.nih.gov/pubmed/33643371
http://dx.doi.org/10.3389/fgene.2020.602608
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author Conceição, Marcos Vinícius Reis
Costa, Sávio Souza
Schaan, Ana Paula
Ribeiro-dos-Santos, Ândrea Kely Campos
Silva, Artur
das Graças, Diego Assis
Schneider, Maria Paula Cruz
Baraúna, Rafael Azevedo
author_facet Conceição, Marcos Vinícius Reis
Costa, Sávio Souza
Schaan, Ana Paula
Ribeiro-dos-Santos, Ândrea Kely Campos
Silva, Artur
das Graças, Diego Assis
Schneider, Maria Paula Cruz
Baraúna, Rafael Azevedo
author_sort Conceição, Marcos Vinícius Reis
collection PubMed
description The mangrove oysters (Crassostrea gasar) are molluscs native to the Amazonia region and their exploration and farming has increased considerably in recent years. These animals are farmed on beds built in the rivers of the Amazonia estuaries and, therefore, the composition of their microbiome should be directly influenced by environmental conditions. Our work aimed to evaluate the changes in bacterial composition of oyster's microbiota at two different seasons (rainy and dry). For this purpose, we amplified and sequenced the V3-V4 regions of the 16S rRNA gene. Sequencing was performed on the Illumina MiSeq platform. According to the rarefaction curve, the sampling effort was sufficient to describe the bacterial diversity in the samples. Alpha-diversity indexes showed that the bacterial microbiota of oysters is richer during the rainy season. This richness is possibly associated with the diversity at lower taxonomic levels, since the relative abundance of bacterial phyla in the two seasons remained relatively constant. The main phyla found include Firmicutes, Bacteroidetes, Actinobacteria, and Proteobacteria. Similar results were found for the species Crassostrea gigas, Crassostrea sikamea, and Crassostrea corteziensis. Beta-diversity analysis showed that the bacterial composition of oyster's gut microbiota was quite different in the two seasons. Our data demonstrate the close relationship between the environment and the microbiome of these molluscs, reinforcing the need for conservation and sustainable management of estuaries in the Amazonia.
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spelling pubmed-79076362021-02-27 Amazonia Seasons Have an Influence in the Composition of Bacterial Gut Microbiota of Mangrove Oysters (Crassostrea gasar) Conceição, Marcos Vinícius Reis Costa, Sávio Souza Schaan, Ana Paula Ribeiro-dos-Santos, Ândrea Kely Campos Silva, Artur das Graças, Diego Assis Schneider, Maria Paula Cruz Baraúna, Rafael Azevedo Front Genet Genetics The mangrove oysters (Crassostrea gasar) are molluscs native to the Amazonia region and their exploration and farming has increased considerably in recent years. These animals are farmed on beds built in the rivers of the Amazonia estuaries and, therefore, the composition of their microbiome should be directly influenced by environmental conditions. Our work aimed to evaluate the changes in bacterial composition of oyster's microbiota at two different seasons (rainy and dry). For this purpose, we amplified and sequenced the V3-V4 regions of the 16S rRNA gene. Sequencing was performed on the Illumina MiSeq platform. According to the rarefaction curve, the sampling effort was sufficient to describe the bacterial diversity in the samples. Alpha-diversity indexes showed that the bacterial microbiota of oysters is richer during the rainy season. This richness is possibly associated with the diversity at lower taxonomic levels, since the relative abundance of bacterial phyla in the two seasons remained relatively constant. The main phyla found include Firmicutes, Bacteroidetes, Actinobacteria, and Proteobacteria. Similar results were found for the species Crassostrea gigas, Crassostrea sikamea, and Crassostrea corteziensis. Beta-diversity analysis showed that the bacterial composition of oyster's gut microbiota was quite different in the two seasons. Our data demonstrate the close relationship between the environment and the microbiome of these molluscs, reinforcing the need for conservation and sustainable management of estuaries in the Amazonia. Frontiers Media S.A. 2021-02-12 /pmc/articles/PMC7907636/ /pubmed/33643371 http://dx.doi.org/10.3389/fgene.2020.602608 Text en Copyright © 2021 Conceição, Costa, Schaan, Ribeiro-dos-Santos, Silva, das Graças, Schneider and Baraúna. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Conceição, Marcos Vinícius Reis
Costa, Sávio Souza
Schaan, Ana Paula
Ribeiro-dos-Santos, Ândrea Kely Campos
Silva, Artur
das Graças, Diego Assis
Schneider, Maria Paula Cruz
Baraúna, Rafael Azevedo
Amazonia Seasons Have an Influence in the Composition of Bacterial Gut Microbiota of Mangrove Oysters (Crassostrea gasar)
title Amazonia Seasons Have an Influence in the Composition of Bacterial Gut Microbiota of Mangrove Oysters (Crassostrea gasar)
title_full Amazonia Seasons Have an Influence in the Composition of Bacterial Gut Microbiota of Mangrove Oysters (Crassostrea gasar)
title_fullStr Amazonia Seasons Have an Influence in the Composition of Bacterial Gut Microbiota of Mangrove Oysters (Crassostrea gasar)
title_full_unstemmed Amazonia Seasons Have an Influence in the Composition of Bacterial Gut Microbiota of Mangrove Oysters (Crassostrea gasar)
title_short Amazonia Seasons Have an Influence in the Composition of Bacterial Gut Microbiota of Mangrove Oysters (Crassostrea gasar)
title_sort amazonia seasons have an influence in the composition of bacterial gut microbiota of mangrove oysters (crassostrea gasar)
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7907636/
https://www.ncbi.nlm.nih.gov/pubmed/33643371
http://dx.doi.org/10.3389/fgene.2020.602608
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