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Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation

Increasing resistance towards anthelmintic drugs has necessitated the search for alternative treatments for the control of gastrointestinal nematode parasites. Animals fed on chicory (Cichorium intybus L.), a temperate (pasture) crop, have reduced parasite burdens, hence making C. intybus a potentia...

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Autores principales: Valente, Angela H., de Roode, Matthew, Ernst, Madeleine, Peña-Espinoza, Miguel, Bornancin, Louis, Bonde, Charlotte S., Martínez-Valladares, María, Ramünke, Sabrina, Krücken, Jürgen, Simonsen, Henrik T., Thamsborg, Stig M., Williams, Andrew R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7907819/
https://www.ncbi.nlm.nih.gov/pubmed/33618233
http://dx.doi.org/10.1016/j.ijpddr.2021.02.002
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author Valente, Angela H.
de Roode, Matthew
Ernst, Madeleine
Peña-Espinoza, Miguel
Bornancin, Louis
Bonde, Charlotte S.
Martínez-Valladares, María
Ramünke, Sabrina
Krücken, Jürgen
Simonsen, Henrik T.
Thamsborg, Stig M.
Williams, Andrew R.
author_facet Valente, Angela H.
de Roode, Matthew
Ernst, Madeleine
Peña-Espinoza, Miguel
Bornancin, Louis
Bonde, Charlotte S.
Martínez-Valladares, María
Ramünke, Sabrina
Krücken, Jürgen
Simonsen, Henrik T.
Thamsborg, Stig M.
Williams, Andrew R.
author_sort Valente, Angela H.
collection PubMed
description Increasing resistance towards anthelmintic drugs has necessitated the search for alternative treatments for the control of gastrointestinal nematode parasites. Animals fed on chicory (Cichorium intybus L.), a temperate (pasture) crop, have reduced parasite burdens, hence making C. intybus a potentially useful source for novel anthelmintic compounds or a diet-based preventive/therapeutic option. Here, we utilized in vitro bioassays with the parasitic nematode Ascaris suum and molecular networking techniques with five chicory cultivars to identify putative active compounds. Network analysis predicted sesquiterpene lactones (SL) as the most likely group of anthelmintic compounds. Further bioassay-guided fractionation supported these predictions, and isolation of pure compounds demonstrated that the SL 8-deoxylactucin (8-DOL) is the compound most strongly associated with anti-parasitic activity. Furthermore, we showed that 8-DOL acts in a synergistic combination with other SL to exert the anti-parasitic effects. Finally, we established that chicory-derived extracts also showed activity against two ruminant nematodes (Teladorsagia circumcincta and Cooperia oncophora) in in vitro assays. Collectively, our results confirm the anti-parasitic activity of chicory against a range of nematodes, and pave the way for targeted extraction of active compounds or selective breeding of specific cultivars to optimize its future use in human and veterinary medicine.
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spelling pubmed-79078192021-03-03 Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation Valente, Angela H. de Roode, Matthew Ernst, Madeleine Peña-Espinoza, Miguel Bornancin, Louis Bonde, Charlotte S. Martínez-Valladares, María Ramünke, Sabrina Krücken, Jürgen Simonsen, Henrik T. Thamsborg, Stig M. Williams, Andrew R. Int J Parasitol Drugs Drug Resist Regular article Increasing resistance towards anthelmintic drugs has necessitated the search for alternative treatments for the control of gastrointestinal nematode parasites. Animals fed on chicory (Cichorium intybus L.), a temperate (pasture) crop, have reduced parasite burdens, hence making C. intybus a potentially useful source for novel anthelmintic compounds or a diet-based preventive/therapeutic option. Here, we utilized in vitro bioassays with the parasitic nematode Ascaris suum and molecular networking techniques with five chicory cultivars to identify putative active compounds. Network analysis predicted sesquiterpene lactones (SL) as the most likely group of anthelmintic compounds. Further bioassay-guided fractionation supported these predictions, and isolation of pure compounds demonstrated that the SL 8-deoxylactucin (8-DOL) is the compound most strongly associated with anti-parasitic activity. Furthermore, we showed that 8-DOL acts in a synergistic combination with other SL to exert the anti-parasitic effects. Finally, we established that chicory-derived extracts also showed activity against two ruminant nematodes (Teladorsagia circumcincta and Cooperia oncophora) in in vitro assays. Collectively, our results confirm the anti-parasitic activity of chicory against a range of nematodes, and pave the way for targeted extraction of active compounds or selective breeding of specific cultivars to optimize its future use in human and veterinary medicine. Elsevier 2021-02-11 /pmc/articles/PMC7907819/ /pubmed/33618233 http://dx.doi.org/10.1016/j.ijpddr.2021.02.002 Text en © 2021 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Regular article
Valente, Angela H.
de Roode, Matthew
Ernst, Madeleine
Peña-Espinoza, Miguel
Bornancin, Louis
Bonde, Charlotte S.
Martínez-Valladares, María
Ramünke, Sabrina
Krücken, Jürgen
Simonsen, Henrik T.
Thamsborg, Stig M.
Williams, Andrew R.
Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation
title Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation
title_full Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation
title_fullStr Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation
title_full_unstemmed Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation
title_short Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation
title_sort identification of compounds responsible for the anthelmintic effects of chicory (cichorium intybus) by molecular networking and bio-guided fractionation
topic Regular article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7907819/
https://www.ncbi.nlm.nih.gov/pubmed/33618233
http://dx.doi.org/10.1016/j.ijpddr.2021.02.002
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