Similar responsiveness between C57BL/6N and C57BL/6J mouse substrains to superovulation

Superovulation is a method for the drug-induced release of multiple eggs and useful for in vitro fertilization. Thus, its high efficiency largely reduces the number of mice used per experiment. We compared the responsivity to superovulation between C57BL/6N (B6N) and C57BL/6J (B6J) substrains. The a...

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Autores principales: Shindo, Miyuki, Tsumura, Hideki, Miyado, Kenji, Kang, Woojin, Kawano, Natsuko, Yoshida, Tomoko, Fukami, Maki, Miyado, Mami
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2021
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7907840/
https://www.ncbi.nlm.nih.gov/pubmed/33655202
http://dx.doi.org/10.17912/micropub.biology.000375
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author Shindo, Miyuki
Tsumura, Hideki
Miyado, Kenji
Kang, Woojin
Kawano, Natsuko
Yoshida, Tomoko
Fukami, Maki
Miyado, Mami
author_facet Shindo, Miyuki
Tsumura, Hideki
Miyado, Kenji
Kang, Woojin
Kawano, Natsuko
Yoshida, Tomoko
Fukami, Maki
Miyado, Mami
author_sort Shindo, Miyuki
collection PubMed
description Superovulation is a method for the drug-induced release of multiple eggs and useful for in vitro fertilization. Thus, its high efficiency largely reduces the number of mice used per experiment. We compared the responsivity to superovulation between C57BL/6N (B6N) and C57BL/6J (B6J) substrains. The average number of ovulated eggs was strikingly higher in both substrains treated with anti-inhibin serum (AIS) plus equine chorionic gonadotropin (eCG) than those treated with eCG alone. Our data indicate that hypothalamus–pituitary–ovarian axis similarly responds to eCG treatment in B6N and B6J mice, and that this responsiveness is enhanced by the presence of AIS.
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spelling pubmed-79078402021-03-01 Similar responsiveness between C57BL/6N and C57BL/6J mouse substrains to superovulation Shindo, Miyuki Tsumura, Hideki Miyado, Kenji Kang, Woojin Kawano, Natsuko Yoshida, Tomoko Fukami, Maki Miyado, Mami MicroPubl Biol New Finding Superovulation is a method for the drug-induced release of multiple eggs and useful for in vitro fertilization. Thus, its high efficiency largely reduces the number of mice used per experiment. We compared the responsivity to superovulation between C57BL/6N (B6N) and C57BL/6J (B6J) substrains. The average number of ovulated eggs was strikingly higher in both substrains treated with anti-inhibin serum (AIS) plus equine chorionic gonadotropin (eCG) than those treated with eCG alone. Our data indicate that hypothalamus–pituitary–ovarian axis similarly responds to eCG treatment in B6N and B6J mice, and that this responsiveness is enhanced by the presence of AIS. Caltech Library 2021-02-25 /pmc/articles/PMC7907840/ /pubmed/33655202 http://dx.doi.org/10.17912/micropub.biology.000375 Text en Copyright: © 2021 by the authors https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Shindo, Miyuki
Tsumura, Hideki
Miyado, Kenji
Kang, Woojin
Kawano, Natsuko
Yoshida, Tomoko
Fukami, Maki
Miyado, Mami
Similar responsiveness between C57BL/6N and C57BL/6J mouse substrains to superovulation
title Similar responsiveness between C57BL/6N and C57BL/6J mouse substrains to superovulation
title_full Similar responsiveness between C57BL/6N and C57BL/6J mouse substrains to superovulation
title_fullStr Similar responsiveness between C57BL/6N and C57BL/6J mouse substrains to superovulation
title_full_unstemmed Similar responsiveness between C57BL/6N and C57BL/6J mouse substrains to superovulation
title_short Similar responsiveness between C57BL/6N and C57BL/6J mouse substrains to superovulation
title_sort similar responsiveness between c57bl/6n and c57bl/6j mouse substrains to superovulation
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7907840/
https://www.ncbi.nlm.nih.gov/pubmed/33655202
http://dx.doi.org/10.17912/micropub.biology.000375
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