Analysis of T cells in mouse lymphoid tissue and blood with flow cytometry

T cells play a key role in adaptive immunity. Defects in specific T cell receptors or signaling proteins can alter their frequency and activation status, which may be associated with immune disease or cancer. Monitoring of T cell frequency and function in genetically modified mice or murine models o...

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Autores principales: Skordos, Ioannis, Demeyer, Annelies, Beyaert, Rudi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7907922/
https://www.ncbi.nlm.nih.gov/pubmed/33665631
http://dx.doi.org/10.1016/j.xpro.2021.100351
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author Skordos, Ioannis
Demeyer, Annelies
Beyaert, Rudi
author_facet Skordos, Ioannis
Demeyer, Annelies
Beyaert, Rudi
author_sort Skordos, Ioannis
collection PubMed
description T cells play a key role in adaptive immunity. Defects in specific T cell receptors or signaling proteins can alter their frequency and activation status, which may be associated with immune disease or cancer. Monitoring of T cell frequency and function in genetically modified mice or murine models of disease is therefore of high interest. Here, we provide a detailed protocol to analyze regulatory T cells, T cell activation, and cytokine production in thymus, spleen, or blood via flow cytometry. For complete details on the use and execution of this protocol, please refer to Demeyer et al. (2020).
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spelling pubmed-79079222021-03-03 Analysis of T cells in mouse lymphoid tissue and blood with flow cytometry Skordos, Ioannis Demeyer, Annelies Beyaert, Rudi STAR Protoc Protocol T cells play a key role in adaptive immunity. Defects in specific T cell receptors or signaling proteins can alter their frequency and activation status, which may be associated with immune disease or cancer. Monitoring of T cell frequency and function in genetically modified mice or murine models of disease is therefore of high interest. Here, we provide a detailed protocol to analyze regulatory T cells, T cell activation, and cytokine production in thymus, spleen, or blood via flow cytometry. For complete details on the use and execution of this protocol, please refer to Demeyer et al. (2020). Elsevier 2021-02-20 /pmc/articles/PMC7907922/ /pubmed/33665631 http://dx.doi.org/10.1016/j.xpro.2021.100351 Text en © 2021 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Skordos, Ioannis
Demeyer, Annelies
Beyaert, Rudi
Analysis of T cells in mouse lymphoid tissue and blood with flow cytometry
title Analysis of T cells in mouse lymphoid tissue and blood with flow cytometry
title_full Analysis of T cells in mouse lymphoid tissue and blood with flow cytometry
title_fullStr Analysis of T cells in mouse lymphoid tissue and blood with flow cytometry
title_full_unstemmed Analysis of T cells in mouse lymphoid tissue and blood with flow cytometry
title_short Analysis of T cells in mouse lymphoid tissue and blood with flow cytometry
title_sort analysis of t cells in mouse lymphoid tissue and blood with flow cytometry
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7907922/
https://www.ncbi.nlm.nih.gov/pubmed/33665631
http://dx.doi.org/10.1016/j.xpro.2021.100351
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