Cargando…
Enzymatic Hydrolysate of Cinnamon Waste Material as Feedstock for the Microbial Production of Carotenoids
In the context of the global need to move towards circular economies, microbial cell factories can be employed thanks to their ability to use side-stream biomasses from the agro-industrial sector to obtain additional products. The valorization of residues allows for better and complete use of natura...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7908450/ https://www.ncbi.nlm.nih.gov/pubmed/33525450 http://dx.doi.org/10.3390/ijerph18031146 |
_version_ | 1783655716636065792 |
---|---|
author | Bertacchi, Stefano Pagliari, Stefania Cantù, Chiara Bruni, Ilaria Labra, Massimo Branduardi, Paola |
author_facet | Bertacchi, Stefano Pagliari, Stefania Cantù, Chiara Bruni, Ilaria Labra, Massimo Branduardi, Paola |
author_sort | Bertacchi, Stefano |
collection | PubMed |
description | In the context of the global need to move towards circular economies, microbial cell factories can be employed thanks to their ability to use side-stream biomasses from the agro-industrial sector to obtain additional products. The valorization of residues allows for better and complete use of natural resources and, at the same time, for the avoidance of waste management to address our needs. In this work, we focused our attention on the microbial valorization of cinnamon waste material after polyphenol extraction (C-PEW) (Cinnamomum verum J.Presl), generally discarded without any additional processing. The sugars embedded in C-PEW were released by enzymatic hydrolysis, more compatible than acid hydrolysis with the subsequent microbial cultivation. We demonstrated that the yeast Rhodosporidium toruloides was able to grow and produce up to 2.00 (±0.23) mg/L of carotenoids in the resulting hydrolysate as a sole carbon and nitrogen source despite the presence of antimicrobial compounds typical of cinnamon. To further extend the potential of our finding, we tested other fungal cell factories for growth on the same media. Overall, these results are opening the possibility to develop separate hydrolysis and fermentation (SHF) bioprocesses based on C-PEW and microbial biotransformation to obtain high-value molecules. |
format | Online Article Text |
id | pubmed-7908450 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79084502021-02-27 Enzymatic Hydrolysate of Cinnamon Waste Material as Feedstock for the Microbial Production of Carotenoids Bertacchi, Stefano Pagliari, Stefania Cantù, Chiara Bruni, Ilaria Labra, Massimo Branduardi, Paola Int J Environ Res Public Health Article In the context of the global need to move towards circular economies, microbial cell factories can be employed thanks to their ability to use side-stream biomasses from the agro-industrial sector to obtain additional products. The valorization of residues allows for better and complete use of natural resources and, at the same time, for the avoidance of waste management to address our needs. In this work, we focused our attention on the microbial valorization of cinnamon waste material after polyphenol extraction (C-PEW) (Cinnamomum verum J.Presl), generally discarded without any additional processing. The sugars embedded in C-PEW were released by enzymatic hydrolysis, more compatible than acid hydrolysis with the subsequent microbial cultivation. We demonstrated that the yeast Rhodosporidium toruloides was able to grow and produce up to 2.00 (±0.23) mg/L of carotenoids in the resulting hydrolysate as a sole carbon and nitrogen source despite the presence of antimicrobial compounds typical of cinnamon. To further extend the potential of our finding, we tested other fungal cell factories for growth on the same media. Overall, these results are opening the possibility to develop separate hydrolysis and fermentation (SHF) bioprocesses based on C-PEW and microbial biotransformation to obtain high-value molecules. MDPI 2021-01-28 2021-02 /pmc/articles/PMC7908450/ /pubmed/33525450 http://dx.doi.org/10.3390/ijerph18031146 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bertacchi, Stefano Pagliari, Stefania Cantù, Chiara Bruni, Ilaria Labra, Massimo Branduardi, Paola Enzymatic Hydrolysate of Cinnamon Waste Material as Feedstock for the Microbial Production of Carotenoids |
title | Enzymatic Hydrolysate of Cinnamon Waste Material as Feedstock for the Microbial Production of Carotenoids |
title_full | Enzymatic Hydrolysate of Cinnamon Waste Material as Feedstock for the Microbial Production of Carotenoids |
title_fullStr | Enzymatic Hydrolysate of Cinnamon Waste Material as Feedstock for the Microbial Production of Carotenoids |
title_full_unstemmed | Enzymatic Hydrolysate of Cinnamon Waste Material as Feedstock for the Microbial Production of Carotenoids |
title_short | Enzymatic Hydrolysate of Cinnamon Waste Material as Feedstock for the Microbial Production of Carotenoids |
title_sort | enzymatic hydrolysate of cinnamon waste material as feedstock for the microbial production of carotenoids |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7908450/ https://www.ncbi.nlm.nih.gov/pubmed/33525450 http://dx.doi.org/10.3390/ijerph18031146 |
work_keys_str_mv | AT bertacchistefano enzymatichydrolysateofcinnamonwastematerialasfeedstockforthemicrobialproductionofcarotenoids AT pagliaristefania enzymatichydrolysateofcinnamonwastematerialasfeedstockforthemicrobialproductionofcarotenoids AT cantuchiara enzymatichydrolysateofcinnamonwastematerialasfeedstockforthemicrobialproductionofcarotenoids AT bruniilaria enzymatichydrolysateofcinnamonwastematerialasfeedstockforthemicrobialproductionofcarotenoids AT labramassimo enzymatichydrolysateofcinnamonwastematerialasfeedstockforthemicrobialproductionofcarotenoids AT branduardipaola enzymatichydrolysateofcinnamonwastematerialasfeedstockforthemicrobialproductionofcarotenoids |