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Enzymatic Hydrolysate of Cinnamon Waste Material as Feedstock for the Microbial Production of Carotenoids

In the context of the global need to move towards circular economies, microbial cell factories can be employed thanks to their ability to use side-stream biomasses from the agro-industrial sector to obtain additional products. The valorization of residues allows for better and complete use of natura...

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Autores principales: Bertacchi, Stefano, Pagliari, Stefania, Cantù, Chiara, Bruni, Ilaria, Labra, Massimo, Branduardi, Paola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7908450/
https://www.ncbi.nlm.nih.gov/pubmed/33525450
http://dx.doi.org/10.3390/ijerph18031146
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author Bertacchi, Stefano
Pagliari, Stefania
Cantù, Chiara
Bruni, Ilaria
Labra, Massimo
Branduardi, Paola
author_facet Bertacchi, Stefano
Pagliari, Stefania
Cantù, Chiara
Bruni, Ilaria
Labra, Massimo
Branduardi, Paola
author_sort Bertacchi, Stefano
collection PubMed
description In the context of the global need to move towards circular economies, microbial cell factories can be employed thanks to their ability to use side-stream biomasses from the agro-industrial sector to obtain additional products. The valorization of residues allows for better and complete use of natural resources and, at the same time, for the avoidance of waste management to address our needs. In this work, we focused our attention on the microbial valorization of cinnamon waste material after polyphenol extraction (C-PEW) (Cinnamomum verum J.Presl), generally discarded without any additional processing. The sugars embedded in C-PEW were released by enzymatic hydrolysis, more compatible than acid hydrolysis with the subsequent microbial cultivation. We demonstrated that the yeast Rhodosporidium toruloides was able to grow and produce up to 2.00 (±0.23) mg/L of carotenoids in the resulting hydrolysate as a sole carbon and nitrogen source despite the presence of antimicrobial compounds typical of cinnamon. To further extend the potential of our finding, we tested other fungal cell factories for growth on the same media. Overall, these results are opening the possibility to develop separate hydrolysis and fermentation (SHF) bioprocesses based on C-PEW and microbial biotransformation to obtain high-value molecules.
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spelling pubmed-79084502021-02-27 Enzymatic Hydrolysate of Cinnamon Waste Material as Feedstock for the Microbial Production of Carotenoids Bertacchi, Stefano Pagliari, Stefania Cantù, Chiara Bruni, Ilaria Labra, Massimo Branduardi, Paola Int J Environ Res Public Health Article In the context of the global need to move towards circular economies, microbial cell factories can be employed thanks to their ability to use side-stream biomasses from the agro-industrial sector to obtain additional products. The valorization of residues allows for better and complete use of natural resources and, at the same time, for the avoidance of waste management to address our needs. In this work, we focused our attention on the microbial valorization of cinnamon waste material after polyphenol extraction (C-PEW) (Cinnamomum verum J.Presl), generally discarded without any additional processing. The sugars embedded in C-PEW were released by enzymatic hydrolysis, more compatible than acid hydrolysis with the subsequent microbial cultivation. We demonstrated that the yeast Rhodosporidium toruloides was able to grow and produce up to 2.00 (±0.23) mg/L of carotenoids in the resulting hydrolysate as a sole carbon and nitrogen source despite the presence of antimicrobial compounds typical of cinnamon. To further extend the potential of our finding, we tested other fungal cell factories for growth on the same media. Overall, these results are opening the possibility to develop separate hydrolysis and fermentation (SHF) bioprocesses based on C-PEW and microbial biotransformation to obtain high-value molecules. MDPI 2021-01-28 2021-02 /pmc/articles/PMC7908450/ /pubmed/33525450 http://dx.doi.org/10.3390/ijerph18031146 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bertacchi, Stefano
Pagliari, Stefania
Cantù, Chiara
Bruni, Ilaria
Labra, Massimo
Branduardi, Paola
Enzymatic Hydrolysate of Cinnamon Waste Material as Feedstock for the Microbial Production of Carotenoids
title Enzymatic Hydrolysate of Cinnamon Waste Material as Feedstock for the Microbial Production of Carotenoids
title_full Enzymatic Hydrolysate of Cinnamon Waste Material as Feedstock for the Microbial Production of Carotenoids
title_fullStr Enzymatic Hydrolysate of Cinnamon Waste Material as Feedstock for the Microbial Production of Carotenoids
title_full_unstemmed Enzymatic Hydrolysate of Cinnamon Waste Material as Feedstock for the Microbial Production of Carotenoids
title_short Enzymatic Hydrolysate of Cinnamon Waste Material as Feedstock for the Microbial Production of Carotenoids
title_sort enzymatic hydrolysate of cinnamon waste material as feedstock for the microbial production of carotenoids
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7908450/
https://www.ncbi.nlm.nih.gov/pubmed/33525450
http://dx.doi.org/10.3390/ijerph18031146
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