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The long non-coding RNA MEG3 plays critical roles in the pathogenesis of cholesterol gallstone
BACKGROUND: Cholesterol gallstone (CG) is the most common gallstone disease, which is induced by biliary cholesterol supersaturation. The purpose of this study is to investigate the pathogenesis of CG. METHODS: Sixteen mice were equally and randomly divided into model group and normal control group....
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7908887/ https://www.ncbi.nlm.nih.gov/pubmed/33665015 http://dx.doi.org/10.7717/peerj.10803 |
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author | Qian, Changlin Qiu, Weiqing Zhang, Jie Shen, Zhiyong Liu, Hua Zhang, Yongjie |
author_facet | Qian, Changlin Qiu, Weiqing Zhang, Jie Shen, Zhiyong Liu, Hua Zhang, Yongjie |
author_sort | Qian, Changlin |
collection | PubMed |
description | BACKGROUND: Cholesterol gallstone (CG) is the most common gallstone disease, which is induced by biliary cholesterol supersaturation. The purpose of this study is to investigate the pathogenesis of CG. METHODS: Sixteen mice were equally and randomly divided into model group and normal control group. The model group was fed with lithogenic diets to induce CG, and then gallbladder bile lipid analysis was performed. After RNA-seq library was constructed, differentially expressed mRNAs (DE-mRNAs) and differentially expressed lncRNAs (DE-lncRNAs) between model group and normal control group were analyzed by DESeq2 package. Using the cluster Profiler package, enrichment analysis for the DE-mRNAs was carried out. Based on Cytoscape software, the protein-protein interaction (PPI) network and competing endogenous RNA (ceRNA) network were built. Using quantitative real-time reverse transcription-PCR (qRT-PCR) analysis, the key RNAs were validated. RESULTS: The mouse model of CG was suc cessfully established, and then 181 DE-mRNAs and 33 DE-lncRNAs between model and normal groups were obtained. Moreover, KDM4A was selected as a hub node in the PPI network, and lncRNA MEG3 was considered as a key lncRNA in the regulatory network. Additionally, the miR-107-5p/miR-149-3p/miR-346-3-MEG3 regulatory pairs and MEG3-PABPC4/CEP131/NUMB1 co-expression pairs existed in the regulatory network. The qRT-PCR analysis showed that KDM4A expression was increased, and the expressions of MEG3, PABPC4, CEP131, and NUMB1 were downregulated. CONCLUSION: These RNAs might be related to the pathogenesis of CG. |
format | Online Article Text |
id | pubmed-7908887 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79088872021-03-03 The long non-coding RNA MEG3 plays critical roles in the pathogenesis of cholesterol gallstone Qian, Changlin Qiu, Weiqing Zhang, Jie Shen, Zhiyong Liu, Hua Zhang, Yongjie PeerJ Biochemistry BACKGROUND: Cholesterol gallstone (CG) is the most common gallstone disease, which is induced by biliary cholesterol supersaturation. The purpose of this study is to investigate the pathogenesis of CG. METHODS: Sixteen mice were equally and randomly divided into model group and normal control group. The model group was fed with lithogenic diets to induce CG, and then gallbladder bile lipid analysis was performed. After RNA-seq library was constructed, differentially expressed mRNAs (DE-mRNAs) and differentially expressed lncRNAs (DE-lncRNAs) between model group and normal control group were analyzed by DESeq2 package. Using the cluster Profiler package, enrichment analysis for the DE-mRNAs was carried out. Based on Cytoscape software, the protein-protein interaction (PPI) network and competing endogenous RNA (ceRNA) network were built. Using quantitative real-time reverse transcription-PCR (qRT-PCR) analysis, the key RNAs were validated. RESULTS: The mouse model of CG was suc cessfully established, and then 181 DE-mRNAs and 33 DE-lncRNAs between model and normal groups were obtained. Moreover, KDM4A was selected as a hub node in the PPI network, and lncRNA MEG3 was considered as a key lncRNA in the regulatory network. Additionally, the miR-107-5p/miR-149-3p/miR-346-3-MEG3 regulatory pairs and MEG3-PABPC4/CEP131/NUMB1 co-expression pairs existed in the regulatory network. The qRT-PCR analysis showed that KDM4A expression was increased, and the expressions of MEG3, PABPC4, CEP131, and NUMB1 were downregulated. CONCLUSION: These RNAs might be related to the pathogenesis of CG. PeerJ Inc. 2021-02-23 /pmc/articles/PMC7908887/ /pubmed/33665015 http://dx.doi.org/10.7717/peerj.10803 Text en ©2021 Qian et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Biochemistry Qian, Changlin Qiu, Weiqing Zhang, Jie Shen, Zhiyong Liu, Hua Zhang, Yongjie The long non-coding RNA MEG3 plays critical roles in the pathogenesis of cholesterol gallstone |
title | The long non-coding RNA MEG3 plays critical roles in the pathogenesis of cholesterol gallstone |
title_full | The long non-coding RNA MEG3 plays critical roles in the pathogenesis of cholesterol gallstone |
title_fullStr | The long non-coding RNA MEG3 plays critical roles in the pathogenesis of cholesterol gallstone |
title_full_unstemmed | The long non-coding RNA MEG3 plays critical roles in the pathogenesis of cholesterol gallstone |
title_short | The long non-coding RNA MEG3 plays critical roles in the pathogenesis of cholesterol gallstone |
title_sort | long non-coding rna meg3 plays critical roles in the pathogenesis of cholesterol gallstone |
topic | Biochemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7908887/ https://www.ncbi.nlm.nih.gov/pubmed/33665015 http://dx.doi.org/10.7717/peerj.10803 |
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