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Cocktail Protocol for Preparation of Platelet-Rich Fibrin Glue for Autologous Use

BACKGROUND: Biomaterials containing platelets have been used to promote healing of ulcers and burns, as well as in implantology and maxillofacial and plastic surgery to achieve wound healing and tissue repair. Commercial devices to prepare autologous biomaterials involve diverse preparation methods...

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Detalles Bibliográficos
Autores principales: Chenna, Deepika, Shastry, Shamee, Das, Soumya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Penerbit Universiti Sains Malaysia 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7909360/
https://www.ncbi.nlm.nih.gov/pubmed/33679218
http://dx.doi.org/10.21315/mjms2021.28.1.5
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author Chenna, Deepika
Shastry, Shamee
Das, Soumya
author_facet Chenna, Deepika
Shastry, Shamee
Das, Soumya
author_sort Chenna, Deepika
collection PubMed
description BACKGROUND: Biomaterials containing platelets have been used to promote healing of ulcers and burns, as well as in implantology and maxillofacial and plastic surgery to achieve wound healing and tissue repair. Commercial devices to prepare autologous biomaterials involve diverse preparation methods that can have high production costs and low yields. Hence, we designed a protocol for preparation of large amounts of autologous platelet-rich fibrin (PRF) glue using conventional processing techniques for blood components. METHODS: Autologous whole blood collected 72 h before surgery was processed to prepare platelet concentrates and cryoprecipitate. In a closed system, calcium was added to the cryoprecipitate to release autologous thrombin and generate a firm fibrin clot. The fibrin clot, platelets and calcium were then placed in a conical flask in which a PRF glue formed. The protocol was validated through determination of pre- and post-platelet counts and fibrinogen amounts in the product. RESULTS: Platelets were recovered with 68% efficiency during the preparation. Essentially no platelets or fibrinogen were found in the supernatant of the PRF glue, suggesting that nearly all had been incorporated in a PRF glue having a relatively large (8 cm × 10 cm) size. CONCLUSION: The protocol described here is a cost-effective, simple and closed system that can be used to produce large-size PRF glue to promote repair of major surgical defects.
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spelling pubmed-79093602021-03-04 Cocktail Protocol for Preparation of Platelet-Rich Fibrin Glue for Autologous Use Chenna, Deepika Shastry, Shamee Das, Soumya Malays J Med Sci Original Article BACKGROUND: Biomaterials containing platelets have been used to promote healing of ulcers and burns, as well as in implantology and maxillofacial and plastic surgery to achieve wound healing and tissue repair. Commercial devices to prepare autologous biomaterials involve diverse preparation methods that can have high production costs and low yields. Hence, we designed a protocol for preparation of large amounts of autologous platelet-rich fibrin (PRF) glue using conventional processing techniques for blood components. METHODS: Autologous whole blood collected 72 h before surgery was processed to prepare platelet concentrates and cryoprecipitate. In a closed system, calcium was added to the cryoprecipitate to release autologous thrombin and generate a firm fibrin clot. The fibrin clot, platelets and calcium were then placed in a conical flask in which a PRF glue formed. The protocol was validated through determination of pre- and post-platelet counts and fibrinogen amounts in the product. RESULTS: Platelets were recovered with 68% efficiency during the preparation. Essentially no platelets or fibrinogen were found in the supernatant of the PRF glue, suggesting that nearly all had been incorporated in a PRF glue having a relatively large (8 cm × 10 cm) size. CONCLUSION: The protocol described here is a cost-effective, simple and closed system that can be used to produce large-size PRF glue to promote repair of major surgical defects. Penerbit Universiti Sains Malaysia 2021-02 2021-02-24 /pmc/articles/PMC7909360/ /pubmed/33679218 http://dx.doi.org/10.21315/mjms2021.28.1.5 Text en © Penerbit Universiti Sains Malaysia, 2021 This work is licensed under the terms of the Creative Commons Attribution (CC BY) (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Original Article
Chenna, Deepika
Shastry, Shamee
Das, Soumya
Cocktail Protocol for Preparation of Platelet-Rich Fibrin Glue for Autologous Use
title Cocktail Protocol for Preparation of Platelet-Rich Fibrin Glue for Autologous Use
title_full Cocktail Protocol for Preparation of Platelet-Rich Fibrin Glue for Autologous Use
title_fullStr Cocktail Protocol for Preparation of Platelet-Rich Fibrin Glue for Autologous Use
title_full_unstemmed Cocktail Protocol for Preparation of Platelet-Rich Fibrin Glue for Autologous Use
title_short Cocktail Protocol for Preparation of Platelet-Rich Fibrin Glue for Autologous Use
title_sort cocktail protocol for preparation of platelet-rich fibrin glue for autologous use
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7909360/
https://www.ncbi.nlm.nih.gov/pubmed/33679218
http://dx.doi.org/10.21315/mjms2021.28.1.5
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