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In Vitro and In Vivo Evaluation of Three Newly Isolated Bacteriophage Candidates, phiEF7H, phiEF14H1, phiEF19G, for Treatment of Enterococcus faecalis Endophthalmitis

Post-operative endophthalmitis caused by Enterococcus spp. progresses rapidly and often results in substantial and irreversible vision loss. Therefore, novel alternative treatments that are effective against enterococcal endophthalmitis are required. Bacteriophage therapy has the potential to be an...

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Autores principales: Kishimoto, Tatsuma, Ishida, Waka, Nasukawa, Tadahiro, Ujihara, Takako, Nakajima, Isana, Suzuki, Takashi, Uchiyama, Jumpei, Todokoro, Daisuke, Daibata, Masanori, Fukushima, Atsuki, Matsuzaki, Shigenobu, Fukuda, Ken
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7909552/
https://www.ncbi.nlm.nih.gov/pubmed/33498561
http://dx.doi.org/10.3390/microorganisms9020212
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author Kishimoto, Tatsuma
Ishida, Waka
Nasukawa, Tadahiro
Ujihara, Takako
Nakajima, Isana
Suzuki, Takashi
Uchiyama, Jumpei
Todokoro, Daisuke
Daibata, Masanori
Fukushima, Atsuki
Matsuzaki, Shigenobu
Fukuda, Ken
author_facet Kishimoto, Tatsuma
Ishida, Waka
Nasukawa, Tadahiro
Ujihara, Takako
Nakajima, Isana
Suzuki, Takashi
Uchiyama, Jumpei
Todokoro, Daisuke
Daibata, Masanori
Fukushima, Atsuki
Matsuzaki, Shigenobu
Fukuda, Ken
author_sort Kishimoto, Tatsuma
collection PubMed
description Post-operative endophthalmitis caused by Enterococcus spp. progresses rapidly and often results in substantial and irreversible vision loss. Therefore, novel alternative treatments that are effective against enterococcal endophthalmitis are required. Bacteriophage therapy has the potential to be an optional therapy for infectious diseases. Therefore, we investigated the therapeutic potential of three newly isolated enterococcal phages, phiEF7H, phiEF14H1, and phiEF19G, in E. faecalis-induced endophthalmitis. These phages could lyse the broad-range E. faecalis, including strains derived from endophthalmitis and vancomycin-resistant E. faecalis in vitro, as determined by the streak test. Morphological and genomic analyses revealed that these phages were classified into the Herelleviridae genus Kochikohdavirus. The whole genomes of these phages contained 143,399, 143,280, and 143,400 bp, respectively. Endophthalmitis was induced in mice by injection of three strains of E. faecalis derived from post-operative endophthalmitis or vancomycin-resistant strains into the vitreous body. The number of viable bacteria and infiltration of neutrophils in the eye were both decreased by intravitreous injection of phiEF7H, phiEF14H1, and phiEF19G 6 h after injection of all E. faecalis strains. Thus, these results suggest that these newly isolated phages may serve as promising candidates for phage therapy against endophthalmitis.
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spelling pubmed-79095522021-02-27 In Vitro and In Vivo Evaluation of Three Newly Isolated Bacteriophage Candidates, phiEF7H, phiEF14H1, phiEF19G, for Treatment of Enterococcus faecalis Endophthalmitis Kishimoto, Tatsuma Ishida, Waka Nasukawa, Tadahiro Ujihara, Takako Nakajima, Isana Suzuki, Takashi Uchiyama, Jumpei Todokoro, Daisuke Daibata, Masanori Fukushima, Atsuki Matsuzaki, Shigenobu Fukuda, Ken Microorganisms Article Post-operative endophthalmitis caused by Enterococcus spp. progresses rapidly and often results in substantial and irreversible vision loss. Therefore, novel alternative treatments that are effective against enterococcal endophthalmitis are required. Bacteriophage therapy has the potential to be an optional therapy for infectious diseases. Therefore, we investigated the therapeutic potential of three newly isolated enterococcal phages, phiEF7H, phiEF14H1, and phiEF19G, in E. faecalis-induced endophthalmitis. These phages could lyse the broad-range E. faecalis, including strains derived from endophthalmitis and vancomycin-resistant E. faecalis in vitro, as determined by the streak test. Morphological and genomic analyses revealed that these phages were classified into the Herelleviridae genus Kochikohdavirus. The whole genomes of these phages contained 143,399, 143,280, and 143,400 bp, respectively. Endophthalmitis was induced in mice by injection of three strains of E. faecalis derived from post-operative endophthalmitis or vancomycin-resistant strains into the vitreous body. The number of viable bacteria and infiltration of neutrophils in the eye were both decreased by intravitreous injection of phiEF7H, phiEF14H1, and phiEF19G 6 h after injection of all E. faecalis strains. Thus, these results suggest that these newly isolated phages may serve as promising candidates for phage therapy against endophthalmitis. MDPI 2021-01-20 /pmc/articles/PMC7909552/ /pubmed/33498561 http://dx.doi.org/10.3390/microorganisms9020212 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kishimoto, Tatsuma
Ishida, Waka
Nasukawa, Tadahiro
Ujihara, Takako
Nakajima, Isana
Suzuki, Takashi
Uchiyama, Jumpei
Todokoro, Daisuke
Daibata, Masanori
Fukushima, Atsuki
Matsuzaki, Shigenobu
Fukuda, Ken
In Vitro and In Vivo Evaluation of Three Newly Isolated Bacteriophage Candidates, phiEF7H, phiEF14H1, phiEF19G, for Treatment of Enterococcus faecalis Endophthalmitis
title In Vitro and In Vivo Evaluation of Three Newly Isolated Bacteriophage Candidates, phiEF7H, phiEF14H1, phiEF19G, for Treatment of Enterococcus faecalis Endophthalmitis
title_full In Vitro and In Vivo Evaluation of Three Newly Isolated Bacteriophage Candidates, phiEF7H, phiEF14H1, phiEF19G, for Treatment of Enterococcus faecalis Endophthalmitis
title_fullStr In Vitro and In Vivo Evaluation of Three Newly Isolated Bacteriophage Candidates, phiEF7H, phiEF14H1, phiEF19G, for Treatment of Enterococcus faecalis Endophthalmitis
title_full_unstemmed In Vitro and In Vivo Evaluation of Three Newly Isolated Bacteriophage Candidates, phiEF7H, phiEF14H1, phiEF19G, for Treatment of Enterococcus faecalis Endophthalmitis
title_short In Vitro and In Vivo Evaluation of Three Newly Isolated Bacteriophage Candidates, phiEF7H, phiEF14H1, phiEF19G, for Treatment of Enterococcus faecalis Endophthalmitis
title_sort in vitro and in vivo evaluation of three newly isolated bacteriophage candidates, phief7h, phief14h1, phief19g, for treatment of enterococcus faecalis endophthalmitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7909552/
https://www.ncbi.nlm.nih.gov/pubmed/33498561
http://dx.doi.org/10.3390/microorganisms9020212
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