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Porous Silicon Biosensor for the Detection of Bacteria through Their Lysate †

Porous silicon (PSi) has been widely used as a biosensor in recent years due to its large surface area and its optical properties. Most PSi biosensors consist in close-ended porous layers, and, because of the diffusion-limited infiltration of the analyte, they lack sensitivity and speed of response....

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Autores principales: Vercauteren, Roselien, Leprince, Audrey, Mahillon, Jacques, Francis, Laurent A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7909573/
https://www.ncbi.nlm.nih.gov/pubmed/33498536
http://dx.doi.org/10.3390/bios11020027
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author Vercauteren, Roselien
Leprince, Audrey
Mahillon, Jacques
Francis, Laurent A.
author_facet Vercauteren, Roselien
Leprince, Audrey
Mahillon, Jacques
Francis, Laurent A.
author_sort Vercauteren, Roselien
collection PubMed
description Porous silicon (PSi) has been widely used as a biosensor in recent years due to its large surface area and its optical properties. Most PSi biosensors consist in close-ended porous layers, and, because of the diffusion-limited infiltration of the analyte, they lack sensitivity and speed of response. In order to overcome these shortcomings, PSi membranes (PSiMs) have been fabricated using electrochemical etching and standard microfabrication techniques. In this work, PSiMs have been used for the optical detection of Bacillus cereus lysate. Before detection, the bacteria are selectively lysed by PlyB221, an endolysin encoded by the bacteriophage Deep-Blue targeting B. cereus. The detection relies on the infiltration of bacterial lysate inside the membrane, which induces a shift of the effective optical thickness. The biosensor was able to detect a B. cereus bacterial lysate, with an initial bacteria concentration of 10(5) colony forming units per mL (CFU/mL), in only 1 h. This proof-of-concept also illustrates the specificity of the lysis before detection. Not only does this detection platform enable the fast detection of bacteria, but the same technique can be extended to other bacteria using selective lysis, as demonstrated by the detection of Staphylococcus epidermidis, selectively lysed by lysostaphin.
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spelling pubmed-79095732021-02-27 Porous Silicon Biosensor for the Detection of Bacteria through Their Lysate † Vercauteren, Roselien Leprince, Audrey Mahillon, Jacques Francis, Laurent A. Biosensors (Basel) Article Porous silicon (PSi) has been widely used as a biosensor in recent years due to its large surface area and its optical properties. Most PSi biosensors consist in close-ended porous layers, and, because of the diffusion-limited infiltration of the analyte, they lack sensitivity and speed of response. In order to overcome these shortcomings, PSi membranes (PSiMs) have been fabricated using electrochemical etching and standard microfabrication techniques. In this work, PSiMs have been used for the optical detection of Bacillus cereus lysate. Before detection, the bacteria are selectively lysed by PlyB221, an endolysin encoded by the bacteriophage Deep-Blue targeting B. cereus. The detection relies on the infiltration of bacterial lysate inside the membrane, which induces a shift of the effective optical thickness. The biosensor was able to detect a B. cereus bacterial lysate, with an initial bacteria concentration of 10(5) colony forming units per mL (CFU/mL), in only 1 h. This proof-of-concept also illustrates the specificity of the lysis before detection. Not only does this detection platform enable the fast detection of bacteria, but the same technique can be extended to other bacteria using selective lysis, as demonstrated by the detection of Staphylococcus epidermidis, selectively lysed by lysostaphin. MDPI 2021-01-20 /pmc/articles/PMC7909573/ /pubmed/33498536 http://dx.doi.org/10.3390/bios11020027 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vercauteren, Roselien
Leprince, Audrey
Mahillon, Jacques
Francis, Laurent A.
Porous Silicon Biosensor for the Detection of Bacteria through Their Lysate †
title Porous Silicon Biosensor for the Detection of Bacteria through Their Lysate †
title_full Porous Silicon Biosensor for the Detection of Bacteria through Their Lysate †
title_fullStr Porous Silicon Biosensor for the Detection of Bacteria through Their Lysate †
title_full_unstemmed Porous Silicon Biosensor for the Detection of Bacteria through Their Lysate †
title_short Porous Silicon Biosensor for the Detection of Bacteria through Their Lysate †
title_sort porous silicon biosensor for the detection of bacteria through their lysate †
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7909573/
https://www.ncbi.nlm.nih.gov/pubmed/33498536
http://dx.doi.org/10.3390/bios11020027
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