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Characterization of Enterococci- and ESBL-Producing Escherichia coli Isolated from Milk of Bovides with Mastitis in Egypt

This study aimed to investigate the prevalence and antimicrobial resistance of enterococci- and ESBL-producing E. coli isolated from milk of bovine mastitis cases in Egypt. Fifty milk samples of dairy animals were collected from localities in the Nile Delta region of Egypt. Isolates were identified...

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Autores principales: Ahmed, Wedad, Neubauer, Heinrich, Tomaso, Herbert, El Hofy, Fatma Ibrahim, Monecke, Stefan, Abd El-Tawab, Ashraf Awad, Hotzel, Helmut
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7909756/
https://www.ncbi.nlm.nih.gov/pubmed/33494211
http://dx.doi.org/10.3390/pathogens10020097
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author Ahmed, Wedad
Neubauer, Heinrich
Tomaso, Herbert
El Hofy, Fatma Ibrahim
Monecke, Stefan
Abd El-Tawab, Ashraf Awad
Hotzel, Helmut
author_facet Ahmed, Wedad
Neubauer, Heinrich
Tomaso, Herbert
El Hofy, Fatma Ibrahim
Monecke, Stefan
Abd El-Tawab, Ashraf Awad
Hotzel, Helmut
author_sort Ahmed, Wedad
collection PubMed
description This study aimed to investigate the prevalence and antimicrobial resistance of enterococci- and ESBL-producing E. coli isolated from milk of bovine mastitis cases in Egypt. Fifty milk samples of dairy animals were collected from localities in the Nile Delta region of Egypt. Isolates were identified using MALDI-TOF MS, and antibiotic susceptibility testing was performed by the broth microdilution method. PCR amplifications were carried out, targeting resistance-associated genes. Seventeen Enterococcus isolates and eight coliform isolates could be cultivated. Vancomycin resistance rate was high in Ent. faecalis. The VITEK 2 system confirmed all E. coli isolates as ESBL-producing. All Ent. faecalis isolates harbored erm(B), tetL and aac-aphD genes. The vanA gene was detected in Ent. faecalis isolate, vanB was found in other Enterococcus, while one isolate of E. casseliflavus exhibited the vanA gene. E. coli isolates exhibited high prevalence of erm(B) and tetL. E. coli isolates were analyzed by DNA microarray analysis. Four isolates were determined by O-serotyping as O8 (n = 1), O86 (n = 2) and O157 (n = 1). H-serotyping resulted in H11, H12, H21 (two isolates each) and one was of H16 type. Different virulence-associated genes were detected in E. coli isolates including lpfA, astA, celB, cma hemL, intI1 and intI2, and the iroN gene was identified by DNA microarray analysis.
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spelling pubmed-79097562021-02-27 Characterization of Enterococci- and ESBL-Producing Escherichia coli Isolated from Milk of Bovides with Mastitis in Egypt Ahmed, Wedad Neubauer, Heinrich Tomaso, Herbert El Hofy, Fatma Ibrahim Monecke, Stefan Abd El-Tawab, Ashraf Awad Hotzel, Helmut Pathogens Communication This study aimed to investigate the prevalence and antimicrobial resistance of enterococci- and ESBL-producing E. coli isolated from milk of bovine mastitis cases in Egypt. Fifty milk samples of dairy animals were collected from localities in the Nile Delta region of Egypt. Isolates were identified using MALDI-TOF MS, and antibiotic susceptibility testing was performed by the broth microdilution method. PCR amplifications were carried out, targeting resistance-associated genes. Seventeen Enterococcus isolates and eight coliform isolates could be cultivated. Vancomycin resistance rate was high in Ent. faecalis. The VITEK 2 system confirmed all E. coli isolates as ESBL-producing. All Ent. faecalis isolates harbored erm(B), tetL and aac-aphD genes. The vanA gene was detected in Ent. faecalis isolate, vanB was found in other Enterococcus, while one isolate of E. casseliflavus exhibited the vanA gene. E. coli isolates exhibited high prevalence of erm(B) and tetL. E. coli isolates were analyzed by DNA microarray analysis. Four isolates were determined by O-serotyping as O8 (n = 1), O86 (n = 2) and O157 (n = 1). H-serotyping resulted in H11, H12, H21 (two isolates each) and one was of H16 type. Different virulence-associated genes were detected in E. coli isolates including lpfA, astA, celB, cma hemL, intI1 and intI2, and the iroN gene was identified by DNA microarray analysis. MDPI 2021-01-21 /pmc/articles/PMC7909756/ /pubmed/33494211 http://dx.doi.org/10.3390/pathogens10020097 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Ahmed, Wedad
Neubauer, Heinrich
Tomaso, Herbert
El Hofy, Fatma Ibrahim
Monecke, Stefan
Abd El-Tawab, Ashraf Awad
Hotzel, Helmut
Characterization of Enterococci- and ESBL-Producing Escherichia coli Isolated from Milk of Bovides with Mastitis in Egypt
title Characterization of Enterococci- and ESBL-Producing Escherichia coli Isolated from Milk of Bovides with Mastitis in Egypt
title_full Characterization of Enterococci- and ESBL-Producing Escherichia coli Isolated from Milk of Bovides with Mastitis in Egypt
title_fullStr Characterization of Enterococci- and ESBL-Producing Escherichia coli Isolated from Milk of Bovides with Mastitis in Egypt
title_full_unstemmed Characterization of Enterococci- and ESBL-Producing Escherichia coli Isolated from Milk of Bovides with Mastitis in Egypt
title_short Characterization of Enterococci- and ESBL-Producing Escherichia coli Isolated from Milk of Bovides with Mastitis in Egypt
title_sort characterization of enterococci- and esbl-producing escherichia coli isolated from milk of bovides with mastitis in egypt
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7909756/
https://www.ncbi.nlm.nih.gov/pubmed/33494211
http://dx.doi.org/10.3390/pathogens10020097
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