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Characterization of Enterococci- and ESBL-Producing Escherichia coli Isolated from Milk of Bovides with Mastitis in Egypt
This study aimed to investigate the prevalence and antimicrobial resistance of enterococci- and ESBL-producing E. coli isolated from milk of bovine mastitis cases in Egypt. Fifty milk samples of dairy animals were collected from localities in the Nile Delta region of Egypt. Isolates were identified...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7909756/ https://www.ncbi.nlm.nih.gov/pubmed/33494211 http://dx.doi.org/10.3390/pathogens10020097 |
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author | Ahmed, Wedad Neubauer, Heinrich Tomaso, Herbert El Hofy, Fatma Ibrahim Monecke, Stefan Abd El-Tawab, Ashraf Awad Hotzel, Helmut |
author_facet | Ahmed, Wedad Neubauer, Heinrich Tomaso, Herbert El Hofy, Fatma Ibrahim Monecke, Stefan Abd El-Tawab, Ashraf Awad Hotzel, Helmut |
author_sort | Ahmed, Wedad |
collection | PubMed |
description | This study aimed to investigate the prevalence and antimicrobial resistance of enterococci- and ESBL-producing E. coli isolated from milk of bovine mastitis cases in Egypt. Fifty milk samples of dairy animals were collected from localities in the Nile Delta region of Egypt. Isolates were identified using MALDI-TOF MS, and antibiotic susceptibility testing was performed by the broth microdilution method. PCR amplifications were carried out, targeting resistance-associated genes. Seventeen Enterococcus isolates and eight coliform isolates could be cultivated. Vancomycin resistance rate was high in Ent. faecalis. The VITEK 2 system confirmed all E. coli isolates as ESBL-producing. All Ent. faecalis isolates harbored erm(B), tetL and aac-aphD genes. The vanA gene was detected in Ent. faecalis isolate, vanB was found in other Enterococcus, while one isolate of E. casseliflavus exhibited the vanA gene. E. coli isolates exhibited high prevalence of erm(B) and tetL. E. coli isolates were analyzed by DNA microarray analysis. Four isolates were determined by O-serotyping as O8 (n = 1), O86 (n = 2) and O157 (n = 1). H-serotyping resulted in H11, H12, H21 (two isolates each) and one was of H16 type. Different virulence-associated genes were detected in E. coli isolates including lpfA, astA, celB, cma hemL, intI1 and intI2, and the iroN gene was identified by DNA microarray analysis. |
format | Online Article Text |
id | pubmed-7909756 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79097562021-02-27 Characterization of Enterococci- and ESBL-Producing Escherichia coli Isolated from Milk of Bovides with Mastitis in Egypt Ahmed, Wedad Neubauer, Heinrich Tomaso, Herbert El Hofy, Fatma Ibrahim Monecke, Stefan Abd El-Tawab, Ashraf Awad Hotzel, Helmut Pathogens Communication This study aimed to investigate the prevalence and antimicrobial resistance of enterococci- and ESBL-producing E. coli isolated from milk of bovine mastitis cases in Egypt. Fifty milk samples of dairy animals were collected from localities in the Nile Delta region of Egypt. Isolates were identified using MALDI-TOF MS, and antibiotic susceptibility testing was performed by the broth microdilution method. PCR amplifications were carried out, targeting resistance-associated genes. Seventeen Enterococcus isolates and eight coliform isolates could be cultivated. Vancomycin resistance rate was high in Ent. faecalis. The VITEK 2 system confirmed all E. coli isolates as ESBL-producing. All Ent. faecalis isolates harbored erm(B), tetL and aac-aphD genes. The vanA gene was detected in Ent. faecalis isolate, vanB was found in other Enterococcus, while one isolate of E. casseliflavus exhibited the vanA gene. E. coli isolates exhibited high prevalence of erm(B) and tetL. E. coli isolates were analyzed by DNA microarray analysis. Four isolates were determined by O-serotyping as O8 (n = 1), O86 (n = 2) and O157 (n = 1). H-serotyping resulted in H11, H12, H21 (two isolates each) and one was of H16 type. Different virulence-associated genes were detected in E. coli isolates including lpfA, astA, celB, cma hemL, intI1 and intI2, and the iroN gene was identified by DNA microarray analysis. MDPI 2021-01-21 /pmc/articles/PMC7909756/ /pubmed/33494211 http://dx.doi.org/10.3390/pathogens10020097 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Ahmed, Wedad Neubauer, Heinrich Tomaso, Herbert El Hofy, Fatma Ibrahim Monecke, Stefan Abd El-Tawab, Ashraf Awad Hotzel, Helmut Characterization of Enterococci- and ESBL-Producing Escherichia coli Isolated from Milk of Bovides with Mastitis in Egypt |
title | Characterization of Enterococci- and ESBL-Producing Escherichia coli Isolated from Milk of Bovides with Mastitis in Egypt |
title_full | Characterization of Enterococci- and ESBL-Producing Escherichia coli Isolated from Milk of Bovides with Mastitis in Egypt |
title_fullStr | Characterization of Enterococci- and ESBL-Producing Escherichia coli Isolated from Milk of Bovides with Mastitis in Egypt |
title_full_unstemmed | Characterization of Enterococci- and ESBL-Producing Escherichia coli Isolated from Milk of Bovides with Mastitis in Egypt |
title_short | Characterization of Enterococci- and ESBL-Producing Escherichia coli Isolated from Milk of Bovides with Mastitis in Egypt |
title_sort | characterization of enterococci- and esbl-producing escherichia coli isolated from milk of bovides with mastitis in egypt |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7909756/ https://www.ncbi.nlm.nih.gov/pubmed/33494211 http://dx.doi.org/10.3390/pathogens10020097 |
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