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A dynein-associated photoreceptor protein prevents ciliary acclimation to blue light
Light-responsive regulation of ciliary motility is known to be conducted through modulation of dyneins, but the mechanism is not fully understood. Here, we report a novel subunit of the two-headed f/I1 inner arm dynein, named DYBLUP, in animal spermatozoa and a unicellular green alga. This subunit c...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Association for the Advancement of Science
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7909887/ https://www.ncbi.nlm.nih.gov/pubmed/33637535 http://dx.doi.org/10.1126/sciadv.abf3621 |
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| author | Kutomi, Osamu Yamamoto, Ryosuke Hirose, Keiko Mizuno, Katsutoshi Nakagiri, Yuuhei Imai, Hiroshi Noga, Akira Obbineni, Jagan Mohan Zimmermann, Noemi Nakajima, Masako Shibata, Daisuke Shibata, Misa Shiba, Kogiku Kita, Masaki Kigoshi, Hideo Tanaka, Yui Yamasaki, Yuya Asahina, Yuma Song, Chihong Nomura, Mami Nomura, Mamoru Nakajima, Ayako Nakachi, Mia Yamada, Lixy Nakazawa, Shiori Sawada, Hitoshi Murata, Kazuyoshi Mitsuoka, Kaoru Ishikawa, Takashi Wakabayashi, Ken-ichi Kon, Takahide Inaba, Kazuo |
| author_facet | Kutomi, Osamu Yamamoto, Ryosuke Hirose, Keiko Mizuno, Katsutoshi Nakagiri, Yuuhei Imai, Hiroshi Noga, Akira Obbineni, Jagan Mohan Zimmermann, Noemi Nakajima, Masako Shibata, Daisuke Shibata, Misa Shiba, Kogiku Kita, Masaki Kigoshi, Hideo Tanaka, Yui Yamasaki, Yuya Asahina, Yuma Song, Chihong Nomura, Mami Nomura, Mamoru Nakajima, Ayako Nakachi, Mia Yamada, Lixy Nakazawa, Shiori Sawada, Hitoshi Murata, Kazuyoshi Mitsuoka, Kaoru Ishikawa, Takashi Wakabayashi, Ken-ichi Kon, Takahide Inaba, Kazuo |
| author_sort | Kutomi, Osamu |
| collection | PubMed |
| description | Light-responsive regulation of ciliary motility is known to be conducted through modulation of dyneins, but the mechanism is not fully understood. Here, we report a novel subunit of the two-headed f/I1 inner arm dynein, named DYBLUP, in animal spermatozoa and a unicellular green alga. This subunit contains a BLUF (sensors of blue light using FAD) domain that appears to directly modulate dynein activity in response to light. DYBLUP (dynein-associated BLUF protein) mediates the connection between the f/I1 motor domain and the tether complex that links the motor to the doublet microtubule. Chlamydomonas lacking the DYBLUP ortholog shows both positive and negative phototaxis but becomes acclimated and attracted to high-intensity blue light. These results suggest a mechanism to avoid toxic strong light via direct photoregulation of dyneins. |
| format | Online Article Text |
| id | pubmed-7909887 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| publisher | American Association for the Advancement of Science |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-79098872021-03-10 A dynein-associated photoreceptor protein prevents ciliary acclimation to blue light Kutomi, Osamu Yamamoto, Ryosuke Hirose, Keiko Mizuno, Katsutoshi Nakagiri, Yuuhei Imai, Hiroshi Noga, Akira Obbineni, Jagan Mohan Zimmermann, Noemi Nakajima, Masako Shibata, Daisuke Shibata, Misa Shiba, Kogiku Kita, Masaki Kigoshi, Hideo Tanaka, Yui Yamasaki, Yuya Asahina, Yuma Song, Chihong Nomura, Mami Nomura, Mamoru Nakajima, Ayako Nakachi, Mia Yamada, Lixy Nakazawa, Shiori Sawada, Hitoshi Murata, Kazuyoshi Mitsuoka, Kaoru Ishikawa, Takashi Wakabayashi, Ken-ichi Kon, Takahide Inaba, Kazuo Sci Adv Research Articles Light-responsive regulation of ciliary motility is known to be conducted through modulation of dyneins, but the mechanism is not fully understood. Here, we report a novel subunit of the two-headed f/I1 inner arm dynein, named DYBLUP, in animal spermatozoa and a unicellular green alga. This subunit contains a BLUF (sensors of blue light using FAD) domain that appears to directly modulate dynein activity in response to light. DYBLUP (dynein-associated BLUF protein) mediates the connection between the f/I1 motor domain and the tether complex that links the motor to the doublet microtubule. Chlamydomonas lacking the DYBLUP ortholog shows both positive and negative phototaxis but becomes acclimated and attracted to high-intensity blue light. These results suggest a mechanism to avoid toxic strong light via direct photoregulation of dyneins. American Association for the Advancement of Science 2021-02-26 /pmc/articles/PMC7909887/ /pubmed/33637535 http://dx.doi.org/10.1126/sciadv.abf3621 Text en Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/ https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
| spellingShingle | Research Articles Kutomi, Osamu Yamamoto, Ryosuke Hirose, Keiko Mizuno, Katsutoshi Nakagiri, Yuuhei Imai, Hiroshi Noga, Akira Obbineni, Jagan Mohan Zimmermann, Noemi Nakajima, Masako Shibata, Daisuke Shibata, Misa Shiba, Kogiku Kita, Masaki Kigoshi, Hideo Tanaka, Yui Yamasaki, Yuya Asahina, Yuma Song, Chihong Nomura, Mami Nomura, Mamoru Nakajima, Ayako Nakachi, Mia Yamada, Lixy Nakazawa, Shiori Sawada, Hitoshi Murata, Kazuyoshi Mitsuoka, Kaoru Ishikawa, Takashi Wakabayashi, Ken-ichi Kon, Takahide Inaba, Kazuo A dynein-associated photoreceptor protein prevents ciliary acclimation to blue light |
| title | A dynein-associated photoreceptor protein prevents ciliary acclimation to blue light |
| title_full | A dynein-associated photoreceptor protein prevents ciliary acclimation to blue light |
| title_fullStr | A dynein-associated photoreceptor protein prevents ciliary acclimation to blue light |
| title_full_unstemmed | A dynein-associated photoreceptor protein prevents ciliary acclimation to blue light |
| title_short | A dynein-associated photoreceptor protein prevents ciliary acclimation to blue light |
| title_sort | dynein-associated photoreceptor protein prevents ciliary acclimation to blue light |
| topic | Research Articles |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7909887/ https://www.ncbi.nlm.nih.gov/pubmed/33637535 http://dx.doi.org/10.1126/sciadv.abf3621 |
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