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Color Fundus Autofluorescence to Determine Activity of Macular Neovascularization in Age-Related Macular Degeneration

PURPOSE: To evaluate with color fundus autofluorescence (FAF) different lesion components of macular neovascularization (MNV) secondary to age-related macular degeneration (AMD) and to assess its activity. METHODS: In total, 137 eyes (102 patients) with MNV underwent a complete eye examination, incl...

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Autores principales: Vujosevic, Stela, Toma, Caterina, Sarao, Valentina, Veritti, Daniele, Brambilla, Marco, Muraca, Andrea, De Cillà, Stefano, Villani, Edoardo, Nucci, Paolo, Lanzetta, Paolo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Association for Research in Vision and Ophthalmology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910646/
https://www.ncbi.nlm.nih.gov/pubmed/34003918
http://dx.doi.org/10.1167/tvst.10.2.33
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author Vujosevic, Stela
Toma, Caterina
Sarao, Valentina
Veritti, Daniele
Brambilla, Marco
Muraca, Andrea
De Cillà, Stefano
Villani, Edoardo
Nucci, Paolo
Lanzetta, Paolo
author_facet Vujosevic, Stela
Toma, Caterina
Sarao, Valentina
Veritti, Daniele
Brambilla, Marco
Muraca, Andrea
De Cillà, Stefano
Villani, Edoardo
Nucci, Paolo
Lanzetta, Paolo
author_sort Vujosevic, Stela
collection PubMed
description PURPOSE: To evaluate with color fundus autofluorescence (FAF) different lesion components of macular neovascularization (MNV) secondary to age-related macular degeneration (AMD) and to assess its activity. METHODS: In total, 137 eyes (102 patients) with MNV underwent a complete eye examination, including color fundus photography, optical coherence tomography (OCT), OCT angiography, and confocal color FAF, with an excitation wavelength at 450 nm. Each image was imported into a custom-image analysis software for quantitative estimation of emission wavelength and green and red emission fluorescence (GEFC/REFC) intensity, considering both single components of neovascular AMD and different MNV types (type 1 and type 2 MNV, active and inactive MNV). RESULTS: Subretinal fluid (SRF) had significantly higher values of GEFC (P = 0.008 and P = 0.0004) and REFC intensity (P = 0.005 and P = 0.0003) versus fibrosis and atrophy. The emission wavelength from SRF was lower compared to atrophy (P = 0.024) but not to fibrosis (P = 0.46). No significant differences were detected between type 1 and 2 MNV. Considering active versus inactive MNVs, a difference was detected for all evaluated parameters (P < 0.001). Mean FAF wavelength of both MNV with SRF and intraretinal fluid (IRF) was lower versus inactive MNV (P < 0.001 and P = 0.005). MNV with SRF (P < 0.001) had higher values of GEFC and REFC versus inactive MNV (P < 0.001). MNV with IRF had higher values of GEFC versus inactive MNV (P = 0.05). CONCLUSIONS: Quantitative color FAF can differentiate active versus inactive MNV, whereas no differences were found between type 1 and type 2 MNV. If these data can be further confirmed, color FAF may be useful for automatic detection of active MNV in AMD and as a guide for treatment. TRANSLATIONAL RELEVANCE: Automatic quantitative evaluation of green and red emission components of FAF in AMD can help determine the activity of MNV and guide the treatment.
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spelling pubmed-79106462021-03-03 Color Fundus Autofluorescence to Determine Activity of Macular Neovascularization in Age-Related Macular Degeneration Vujosevic, Stela Toma, Caterina Sarao, Valentina Veritti, Daniele Brambilla, Marco Muraca, Andrea De Cillà, Stefano Villani, Edoardo Nucci, Paolo Lanzetta, Paolo Transl Vis Sci Technol Article PURPOSE: To evaluate with color fundus autofluorescence (FAF) different lesion components of macular neovascularization (MNV) secondary to age-related macular degeneration (AMD) and to assess its activity. METHODS: In total, 137 eyes (102 patients) with MNV underwent a complete eye examination, including color fundus photography, optical coherence tomography (OCT), OCT angiography, and confocal color FAF, with an excitation wavelength at 450 nm. Each image was imported into a custom-image analysis software for quantitative estimation of emission wavelength and green and red emission fluorescence (GEFC/REFC) intensity, considering both single components of neovascular AMD and different MNV types (type 1 and type 2 MNV, active and inactive MNV). RESULTS: Subretinal fluid (SRF) had significantly higher values of GEFC (P = 0.008 and P = 0.0004) and REFC intensity (P = 0.005 and P = 0.0003) versus fibrosis and atrophy. The emission wavelength from SRF was lower compared to atrophy (P = 0.024) but not to fibrosis (P = 0.46). No significant differences were detected between type 1 and 2 MNV. Considering active versus inactive MNVs, a difference was detected for all evaluated parameters (P < 0.001). Mean FAF wavelength of both MNV with SRF and intraretinal fluid (IRF) was lower versus inactive MNV (P < 0.001 and P = 0.005). MNV with SRF (P < 0.001) had higher values of GEFC and REFC versus inactive MNV (P < 0.001). MNV with IRF had higher values of GEFC versus inactive MNV (P = 0.05). CONCLUSIONS: Quantitative color FAF can differentiate active versus inactive MNV, whereas no differences were found between type 1 and type 2 MNV. If these data can be further confirmed, color FAF may be useful for automatic detection of active MNV in AMD and as a guide for treatment. TRANSLATIONAL RELEVANCE: Automatic quantitative evaluation of green and red emission components of FAF in AMD can help determine the activity of MNV and guide the treatment. The Association for Research in Vision and Ophthalmology 2021-02-22 /pmc/articles/PMC7910646/ /pubmed/34003918 http://dx.doi.org/10.1167/tvst.10.2.33 Text en Copyright 2021 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
spellingShingle Article
Vujosevic, Stela
Toma, Caterina
Sarao, Valentina
Veritti, Daniele
Brambilla, Marco
Muraca, Andrea
De Cillà, Stefano
Villani, Edoardo
Nucci, Paolo
Lanzetta, Paolo
Color Fundus Autofluorescence to Determine Activity of Macular Neovascularization in Age-Related Macular Degeneration
title Color Fundus Autofluorescence to Determine Activity of Macular Neovascularization in Age-Related Macular Degeneration
title_full Color Fundus Autofluorescence to Determine Activity of Macular Neovascularization in Age-Related Macular Degeneration
title_fullStr Color Fundus Autofluorescence to Determine Activity of Macular Neovascularization in Age-Related Macular Degeneration
title_full_unstemmed Color Fundus Autofluorescence to Determine Activity of Macular Neovascularization in Age-Related Macular Degeneration
title_short Color Fundus Autofluorescence to Determine Activity of Macular Neovascularization in Age-Related Macular Degeneration
title_sort color fundus autofluorescence to determine activity of macular neovascularization in age-related macular degeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910646/
https://www.ncbi.nlm.nih.gov/pubmed/34003918
http://dx.doi.org/10.1167/tvst.10.2.33
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