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Plasma Concentrations of Extracellular Vesicles Are Decreased in Patients with Post-Infarct Cardiac Remodelling
SIMPLE SUMMARY: A heart attack may lead to the remodelling of the cardiac muscle, which negatively affects patient’s prognosis. At present, the mechanisms of cardiac remodelling remain unclear. In patients with heart attack, many body cells become activated and release small particles, called extrac...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910841/ https://www.ncbi.nlm.nih.gov/pubmed/33573196 http://dx.doi.org/10.3390/biology10020097 |
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author | Gąsecka, Aleksandra Pluta, Kinga Solarska, Katarzyna Rydz, Bartłomiej Eyileten, Ceren Postula, Marek van der Pol, Edwin Nieuwland, Rienk Budnik, Monika Kochanowski, Janusz Jaguszewski, Miłosz J. Szarpak, Łukasz Mazurek, Tomasz Kapłon-Cieślicka, Agnieszka Opolski, Grzegorz Filipiak, Krzysztof J. |
author_facet | Gąsecka, Aleksandra Pluta, Kinga Solarska, Katarzyna Rydz, Bartłomiej Eyileten, Ceren Postula, Marek van der Pol, Edwin Nieuwland, Rienk Budnik, Monika Kochanowski, Janusz Jaguszewski, Miłosz J. Szarpak, Łukasz Mazurek, Tomasz Kapłon-Cieślicka, Agnieszka Opolski, Grzegorz Filipiak, Krzysztof J. |
author_sort | Gąsecka, Aleksandra |
collection | PubMed |
description | SIMPLE SUMMARY: A heart attack may lead to the remodelling of the cardiac muscle, which negatively affects patient’s prognosis. At present, the mechanisms of cardiac remodelling remain unclear. In patients with heart attack, many body cells become activated and release small particles, called extracellular vesicles, which can either aggravate cardiac injury, or contribute to healing of heart muscle. In our study, we hypothesized that the concentrations of these small particles in plasma allow to determine which patients will experience remodelling of the cardiac muscle after the heart attach. We found that concentrations of extracellular vesicles from endothelial cells, erythrocytes and platelets, measured directly the heart attack, were lower in patients who developed cardiac remodelling 6 months later, compared to patients who had no remodelling. Vesicles from endothelial cells and erythrocytes allowed to determine remodelling independently of other clinical features. Hence, decreased concentrations of these vesicles may on one hand be a sign of inappropriate cardiac repair mechanisms, and on the other hand may allow to identify patients, who will develop cardiac remodelling after the heart attack. ABSTRACT: Background, the mechanisms underlying left ventricular remodelling (LVR) after acute myocardial infarction (AMI) remain obscure. In the course of AMI, blood cells and endothelial cells release extracellular vesicles (EVs). We hypothesized that changes in EV concentrations after AMI may underlie LVR. Methods, plasma concentrations of EVs from endothelial cells (CD146+), erythrocytes (CD235a+), leukocytes (CD45+), platelets (CD61+), activated platelets (P-selectin+), and EVs exposing phosphatidylserine after AMI were determined by flow cytometry in 55 patients with the first AMI. LVR was defined as an increase in left ventricular end-diastolic volume by 20% at 6 months after AMI, compared to baseline. Results, baseline concentrations of EVs from endothelial cells, erythrocytes and platelets were lower in patients who developed LVR (p ≤ 0.02 for all). Concentrations of EVs from endothelial cells and erythrocytes were independent LVR predictors (OR 8.2, CI 1.3–54.2 and OR 17.8, CI 2.3–138.6, respectively) in multivariate analysis. Combining the three EV subtypes allowed to predict LVR with 83% sensitivity and 87% specificity. Conclusions, decreased plasma concentrations of EVs from endothelial cells, erythrocytes and platelets predict LVR after AMI. Since EV release EVs contributes to cellular homeostasis by waste removal, decreased concentrations of EVs may indicate dysfunctional cardiac homeostasis after AMI, thus promoting LVR. |
format | Online Article Text |
id | pubmed-7910841 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79108412021-02-28 Plasma Concentrations of Extracellular Vesicles Are Decreased in Patients with Post-Infarct Cardiac Remodelling Gąsecka, Aleksandra Pluta, Kinga Solarska, Katarzyna Rydz, Bartłomiej Eyileten, Ceren Postula, Marek van der Pol, Edwin Nieuwland, Rienk Budnik, Monika Kochanowski, Janusz Jaguszewski, Miłosz J. Szarpak, Łukasz Mazurek, Tomasz Kapłon-Cieślicka, Agnieszka Opolski, Grzegorz Filipiak, Krzysztof J. Biology (Basel) Article SIMPLE SUMMARY: A heart attack may lead to the remodelling of the cardiac muscle, which negatively affects patient’s prognosis. At present, the mechanisms of cardiac remodelling remain unclear. In patients with heart attack, many body cells become activated and release small particles, called extracellular vesicles, which can either aggravate cardiac injury, or contribute to healing of heart muscle. In our study, we hypothesized that the concentrations of these small particles in plasma allow to determine which patients will experience remodelling of the cardiac muscle after the heart attach. We found that concentrations of extracellular vesicles from endothelial cells, erythrocytes and platelets, measured directly the heart attack, were lower in patients who developed cardiac remodelling 6 months later, compared to patients who had no remodelling. Vesicles from endothelial cells and erythrocytes allowed to determine remodelling independently of other clinical features. Hence, decreased concentrations of these vesicles may on one hand be a sign of inappropriate cardiac repair mechanisms, and on the other hand may allow to identify patients, who will develop cardiac remodelling after the heart attack. ABSTRACT: Background, the mechanisms underlying left ventricular remodelling (LVR) after acute myocardial infarction (AMI) remain obscure. In the course of AMI, blood cells and endothelial cells release extracellular vesicles (EVs). We hypothesized that changes in EV concentrations after AMI may underlie LVR. Methods, plasma concentrations of EVs from endothelial cells (CD146+), erythrocytes (CD235a+), leukocytes (CD45+), platelets (CD61+), activated platelets (P-selectin+), and EVs exposing phosphatidylserine after AMI were determined by flow cytometry in 55 patients with the first AMI. LVR was defined as an increase in left ventricular end-diastolic volume by 20% at 6 months after AMI, compared to baseline. Results, baseline concentrations of EVs from endothelial cells, erythrocytes and platelets were lower in patients who developed LVR (p ≤ 0.02 for all). Concentrations of EVs from endothelial cells and erythrocytes were independent LVR predictors (OR 8.2, CI 1.3–54.2 and OR 17.8, CI 2.3–138.6, respectively) in multivariate analysis. Combining the three EV subtypes allowed to predict LVR with 83% sensitivity and 87% specificity. Conclusions, decreased plasma concentrations of EVs from endothelial cells, erythrocytes and platelets predict LVR after AMI. Since EV release EVs contributes to cellular homeostasis by waste removal, decreased concentrations of EVs may indicate dysfunctional cardiac homeostasis after AMI, thus promoting LVR. MDPI 2021-01-30 /pmc/articles/PMC7910841/ /pubmed/33573196 http://dx.doi.org/10.3390/biology10020097 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gąsecka, Aleksandra Pluta, Kinga Solarska, Katarzyna Rydz, Bartłomiej Eyileten, Ceren Postula, Marek van der Pol, Edwin Nieuwland, Rienk Budnik, Monika Kochanowski, Janusz Jaguszewski, Miłosz J. Szarpak, Łukasz Mazurek, Tomasz Kapłon-Cieślicka, Agnieszka Opolski, Grzegorz Filipiak, Krzysztof J. Plasma Concentrations of Extracellular Vesicles Are Decreased in Patients with Post-Infarct Cardiac Remodelling |
title | Plasma Concentrations of Extracellular Vesicles Are Decreased in Patients with Post-Infarct Cardiac Remodelling |
title_full | Plasma Concentrations of Extracellular Vesicles Are Decreased in Patients with Post-Infarct Cardiac Remodelling |
title_fullStr | Plasma Concentrations of Extracellular Vesicles Are Decreased in Patients with Post-Infarct Cardiac Remodelling |
title_full_unstemmed | Plasma Concentrations of Extracellular Vesicles Are Decreased in Patients with Post-Infarct Cardiac Remodelling |
title_short | Plasma Concentrations of Extracellular Vesicles Are Decreased in Patients with Post-Infarct Cardiac Remodelling |
title_sort | plasma concentrations of extracellular vesicles are decreased in patients with post-infarct cardiac remodelling |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910841/ https://www.ncbi.nlm.nih.gov/pubmed/33573196 http://dx.doi.org/10.3390/biology10020097 |
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