Cargando…

Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai’i Environmental Water Biofilms Compared to Indoor Samples

Nontuberculous mycobacteria (NTM) are environmental organisms that can cause opportunistic pulmonary disease with species diversity showing significant regional variation. In the United States, Hawai’i shows the highest rate of NTM pulmonary disease. The need for improved understanding of NTM reserv...

Descripción completa

Detalles Bibliográficos
Autores principales: Virdi, Ravleen, Lowe, Melissa E., Norton, Grant J., Dawrs, Stephanie N., Hasan, Nabeeh A., Epperson, L. Elaine, Glickman, Cody M., Chan, Edward D., Strong, Michael, Crooks, James L., Honda, Jennifer R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910870/
https://www.ncbi.nlm.nih.gov/pubmed/33499212
http://dx.doi.org/10.3390/microorganisms9020224
_version_ 1783656212874657792
author Virdi, Ravleen
Lowe, Melissa E.
Norton, Grant J.
Dawrs, Stephanie N.
Hasan, Nabeeh A.
Epperson, L. Elaine
Glickman, Cody M.
Chan, Edward D.
Strong, Michael
Crooks, James L.
Honda, Jennifer R.
author_facet Virdi, Ravleen
Lowe, Melissa E.
Norton, Grant J.
Dawrs, Stephanie N.
Hasan, Nabeeh A.
Epperson, L. Elaine
Glickman, Cody M.
Chan, Edward D.
Strong, Michael
Crooks, James L.
Honda, Jennifer R.
author_sort Virdi, Ravleen
collection PubMed
description Nontuberculous mycobacteria (NTM) are environmental organisms that can cause opportunistic pulmonary disease with species diversity showing significant regional variation. In the United States, Hawai’i shows the highest rate of NTM pulmonary disease. The need for improved understanding of NTM reservoirs led us to identify NTM from patient respiratory specimens and compare NTM diversity between outdoor and indoor locations in Hawai’i. A total of 545 water biofilm samples were collected from 357 unique locations across Kaua’i (n = 51), O’ahu (n = 202), Maui (n = 159), and Hawai’i Island (n = 133) and divided into outdoor (n = 179) or indoor (n = 366) categories. rpoB sequence analysis was used to determine NTM species and predictive modeling applied to develop NTM risk maps based on geographic characteristics between environments. M. chimaera was frequently identified from respiratory and environmental samples followed by M. chelonae and M. abscessus; yet significantly less NTM were consistently recovered from outdoor compared to indoor biofilms, as exemplified by showerhead biofilm samples. While the frequency of M. chimaera recovery was comparable between outdoor and indoor showerhead biofilms, phylogenetic analyses demonstrate similar rpoB gene sequences between all showerhead and respiratory M. chimaera isolates, supporting outdoor and indoor environments as possible sources for pulmonary M. chimaera infections.
format Online
Article
Text
id pubmed-7910870
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-79108702021-02-28 Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai’i Environmental Water Biofilms Compared to Indoor Samples Virdi, Ravleen Lowe, Melissa E. Norton, Grant J. Dawrs, Stephanie N. Hasan, Nabeeh A. Epperson, L. Elaine Glickman, Cody M. Chan, Edward D. Strong, Michael Crooks, James L. Honda, Jennifer R. Microorganisms Article Nontuberculous mycobacteria (NTM) are environmental organisms that can cause opportunistic pulmonary disease with species diversity showing significant regional variation. In the United States, Hawai’i shows the highest rate of NTM pulmonary disease. The need for improved understanding of NTM reservoirs led us to identify NTM from patient respiratory specimens and compare NTM diversity between outdoor and indoor locations in Hawai’i. A total of 545 water biofilm samples were collected from 357 unique locations across Kaua’i (n = 51), O’ahu (n = 202), Maui (n = 159), and Hawai’i Island (n = 133) and divided into outdoor (n = 179) or indoor (n = 366) categories. rpoB sequence analysis was used to determine NTM species and predictive modeling applied to develop NTM risk maps based on geographic characteristics between environments. M. chimaera was frequently identified from respiratory and environmental samples followed by M. chelonae and M. abscessus; yet significantly less NTM were consistently recovered from outdoor compared to indoor biofilms, as exemplified by showerhead biofilm samples. While the frequency of M. chimaera recovery was comparable between outdoor and indoor showerhead biofilms, phylogenetic analyses demonstrate similar rpoB gene sequences between all showerhead and respiratory M. chimaera isolates, supporting outdoor and indoor environments as possible sources for pulmonary M. chimaera infections. MDPI 2021-01-22 /pmc/articles/PMC7910870/ /pubmed/33499212 http://dx.doi.org/10.3390/microorganisms9020224 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Virdi, Ravleen
Lowe, Melissa E.
Norton, Grant J.
Dawrs, Stephanie N.
Hasan, Nabeeh A.
Epperson, L. Elaine
Glickman, Cody M.
Chan, Edward D.
Strong, Michael
Crooks, James L.
Honda, Jennifer R.
Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai’i Environmental Water Biofilms Compared to Indoor Samples
title Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai’i Environmental Water Biofilms Compared to Indoor Samples
title_full Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai’i Environmental Water Biofilms Compared to Indoor Samples
title_fullStr Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai’i Environmental Water Biofilms Compared to Indoor Samples
title_full_unstemmed Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai’i Environmental Water Biofilms Compared to Indoor Samples
title_short Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai’i Environmental Water Biofilms Compared to Indoor Samples
title_sort lower recovery of nontuberculous mycobacteria from outdoor hawai’i environmental water biofilms compared to indoor samples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910870/
https://www.ncbi.nlm.nih.gov/pubmed/33499212
http://dx.doi.org/10.3390/microorganisms9020224
work_keys_str_mv AT virdiravleen lowerrecoveryofnontuberculousmycobacteriafromoutdoorhawaiienvironmentalwaterbiofilmscomparedtoindoorsamples
AT lowemelissae lowerrecoveryofnontuberculousmycobacteriafromoutdoorhawaiienvironmentalwaterbiofilmscomparedtoindoorsamples
AT nortongrantj lowerrecoveryofnontuberculousmycobacteriafromoutdoorhawaiienvironmentalwaterbiofilmscomparedtoindoorsamples
AT dawrsstephanien lowerrecoveryofnontuberculousmycobacteriafromoutdoorhawaiienvironmentalwaterbiofilmscomparedtoindoorsamples
AT hasannabeeha lowerrecoveryofnontuberculousmycobacteriafromoutdoorhawaiienvironmentalwaterbiofilmscomparedtoindoorsamples
AT eppersonlelaine lowerrecoveryofnontuberculousmycobacteriafromoutdoorhawaiienvironmentalwaterbiofilmscomparedtoindoorsamples
AT glickmancodym lowerrecoveryofnontuberculousmycobacteriafromoutdoorhawaiienvironmentalwaterbiofilmscomparedtoindoorsamples
AT chanedwardd lowerrecoveryofnontuberculousmycobacteriafromoutdoorhawaiienvironmentalwaterbiofilmscomparedtoindoorsamples
AT strongmichael lowerrecoveryofnontuberculousmycobacteriafromoutdoorhawaiienvironmentalwaterbiofilmscomparedtoindoorsamples
AT crooksjamesl lowerrecoveryofnontuberculousmycobacteriafromoutdoorhawaiienvironmentalwaterbiofilmscomparedtoindoorsamples
AT hondajenniferr lowerrecoveryofnontuberculousmycobacteriafromoutdoorhawaiienvironmentalwaterbiofilmscomparedtoindoorsamples