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Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai’i Environmental Water Biofilms Compared to Indoor Samples
Nontuberculous mycobacteria (NTM) are environmental organisms that can cause opportunistic pulmonary disease with species diversity showing significant regional variation. In the United States, Hawai’i shows the highest rate of NTM pulmonary disease. The need for improved understanding of NTM reserv...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910870/ https://www.ncbi.nlm.nih.gov/pubmed/33499212 http://dx.doi.org/10.3390/microorganisms9020224 |
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author | Virdi, Ravleen Lowe, Melissa E. Norton, Grant J. Dawrs, Stephanie N. Hasan, Nabeeh A. Epperson, L. Elaine Glickman, Cody M. Chan, Edward D. Strong, Michael Crooks, James L. Honda, Jennifer R. |
author_facet | Virdi, Ravleen Lowe, Melissa E. Norton, Grant J. Dawrs, Stephanie N. Hasan, Nabeeh A. Epperson, L. Elaine Glickman, Cody M. Chan, Edward D. Strong, Michael Crooks, James L. Honda, Jennifer R. |
author_sort | Virdi, Ravleen |
collection | PubMed |
description | Nontuberculous mycobacteria (NTM) are environmental organisms that can cause opportunistic pulmonary disease with species diversity showing significant regional variation. In the United States, Hawai’i shows the highest rate of NTM pulmonary disease. The need for improved understanding of NTM reservoirs led us to identify NTM from patient respiratory specimens and compare NTM diversity between outdoor and indoor locations in Hawai’i. A total of 545 water biofilm samples were collected from 357 unique locations across Kaua’i (n = 51), O’ahu (n = 202), Maui (n = 159), and Hawai’i Island (n = 133) and divided into outdoor (n = 179) or indoor (n = 366) categories. rpoB sequence analysis was used to determine NTM species and predictive modeling applied to develop NTM risk maps based on geographic characteristics between environments. M. chimaera was frequently identified from respiratory and environmental samples followed by M. chelonae and M. abscessus; yet significantly less NTM were consistently recovered from outdoor compared to indoor biofilms, as exemplified by showerhead biofilm samples. While the frequency of M. chimaera recovery was comparable between outdoor and indoor showerhead biofilms, phylogenetic analyses demonstrate similar rpoB gene sequences between all showerhead and respiratory M. chimaera isolates, supporting outdoor and indoor environments as possible sources for pulmonary M. chimaera infections. |
format | Online Article Text |
id | pubmed-7910870 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79108702021-02-28 Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai’i Environmental Water Biofilms Compared to Indoor Samples Virdi, Ravleen Lowe, Melissa E. Norton, Grant J. Dawrs, Stephanie N. Hasan, Nabeeh A. Epperson, L. Elaine Glickman, Cody M. Chan, Edward D. Strong, Michael Crooks, James L. Honda, Jennifer R. Microorganisms Article Nontuberculous mycobacteria (NTM) are environmental organisms that can cause opportunistic pulmonary disease with species diversity showing significant regional variation. In the United States, Hawai’i shows the highest rate of NTM pulmonary disease. The need for improved understanding of NTM reservoirs led us to identify NTM from patient respiratory specimens and compare NTM diversity between outdoor and indoor locations in Hawai’i. A total of 545 water biofilm samples were collected from 357 unique locations across Kaua’i (n = 51), O’ahu (n = 202), Maui (n = 159), and Hawai’i Island (n = 133) and divided into outdoor (n = 179) or indoor (n = 366) categories. rpoB sequence analysis was used to determine NTM species and predictive modeling applied to develop NTM risk maps based on geographic characteristics between environments. M. chimaera was frequently identified from respiratory and environmental samples followed by M. chelonae and M. abscessus; yet significantly less NTM were consistently recovered from outdoor compared to indoor biofilms, as exemplified by showerhead biofilm samples. While the frequency of M. chimaera recovery was comparable between outdoor and indoor showerhead biofilms, phylogenetic analyses demonstrate similar rpoB gene sequences between all showerhead and respiratory M. chimaera isolates, supporting outdoor and indoor environments as possible sources for pulmonary M. chimaera infections. MDPI 2021-01-22 /pmc/articles/PMC7910870/ /pubmed/33499212 http://dx.doi.org/10.3390/microorganisms9020224 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Virdi, Ravleen Lowe, Melissa E. Norton, Grant J. Dawrs, Stephanie N. Hasan, Nabeeh A. Epperson, L. Elaine Glickman, Cody M. Chan, Edward D. Strong, Michael Crooks, James L. Honda, Jennifer R. Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai’i Environmental Water Biofilms Compared to Indoor Samples |
title | Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai’i Environmental Water Biofilms Compared to Indoor Samples |
title_full | Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai’i Environmental Water Biofilms Compared to Indoor Samples |
title_fullStr | Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai’i Environmental Water Biofilms Compared to Indoor Samples |
title_full_unstemmed | Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai’i Environmental Water Biofilms Compared to Indoor Samples |
title_short | Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai’i Environmental Water Biofilms Compared to Indoor Samples |
title_sort | lower recovery of nontuberculous mycobacteria from outdoor hawai’i environmental water biofilms compared to indoor samples |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910870/ https://www.ncbi.nlm.nih.gov/pubmed/33499212 http://dx.doi.org/10.3390/microorganisms9020224 |
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