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The Effect of Acute Intense Exercise on Activity of Antioxidant Enzymes in Smokers and Non-Smokers

Acute intense exercise causes significant oxidative stress and consequently an increase in total antioxidant capacity; however, the mechanisms and combined effects of intense exercise and smoking on oxidative stress among active and non-active smokers are not clear. The aim of this study was to inve...

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Autores principales: Nobari, Hadi, Nejad, Hamzeh Abdi, Kargarfard, Mehdi, Mohseni, Soghra, Suzuki, Katsuhiko, Carmelo Adsuar, José, Pérez-Gómez, Jorge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910903/
https://www.ncbi.nlm.nih.gov/pubmed/33513978
http://dx.doi.org/10.3390/biom11020171
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author Nobari, Hadi
Nejad, Hamzeh Abdi
Kargarfard, Mehdi
Mohseni, Soghra
Suzuki, Katsuhiko
Carmelo Adsuar, José
Pérez-Gómez, Jorge
author_facet Nobari, Hadi
Nejad, Hamzeh Abdi
Kargarfard, Mehdi
Mohseni, Soghra
Suzuki, Katsuhiko
Carmelo Adsuar, José
Pérez-Gómez, Jorge
author_sort Nobari, Hadi
collection PubMed
description Acute intense exercise causes significant oxidative stress and consequently an increase in total antioxidant capacity; however, the mechanisms and combined effects of intense exercise and smoking on oxidative stress among active and non-active smokers are not clear. The aim of this study was to investigate the effect of acute intense exercise on antioxidant enzyme activity responses in active and non-active individuals exposed to cigarette smoke. The study included 40 subjects who were equally classified as: smokers that did exercise (SE), smokers that did not do exercise (SnE), non-smokers that did exercise (NSE), and non-smokers that did not do exercise (NSnE). The adjusted Astrand test was used to exhaust the subjects. Salivary enzymes of peroxidase (POX), catalase (CAT), and superoxide dismutase (SOD) were measured, by spectrophotometry methods, at 3 different time points: pre-test (TP1), post-test (TP2), and one hour after finishing the test (TP3). Significant (p < 0.05) group x time interactions were found for the three enzymes. Salivary POX, CAT and SOD increased in all groups from TP1 to TP2 and decreased from TP2 to TP3. Only the NSE showed a significant difference between TP1 to TP3 in POX and SOD by +0.011 ± 0.007 and +0.075 ± 0.020 (U/mL), respectively. The NSE showed significantly higher activity of POX, CAT and SOD in TP2 compared to the other groups. Furthermore, NSE and NSnE had higher activity of POX, CAT and SOD in TP1 and TP3 (p < 0.05) compared with SE and SnE. Only in the NSnE, were no differences observed in CAT compared with SE and SnE in TP3. These results showed that the antioxidant activity at rest and in the recovery time after the acute intense exercise was lower in SE and SnE compared with NSE and NSnE, suggesting that smoking habit may reduce the ameliorating effect of regular physical activity on acute exercise-induced oxidative stress.
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spelling pubmed-79109032021-02-28 The Effect of Acute Intense Exercise on Activity of Antioxidant Enzymes in Smokers and Non-Smokers Nobari, Hadi Nejad, Hamzeh Abdi Kargarfard, Mehdi Mohseni, Soghra Suzuki, Katsuhiko Carmelo Adsuar, José Pérez-Gómez, Jorge Biomolecules Article Acute intense exercise causes significant oxidative stress and consequently an increase in total antioxidant capacity; however, the mechanisms and combined effects of intense exercise and smoking on oxidative stress among active and non-active smokers are not clear. The aim of this study was to investigate the effect of acute intense exercise on antioxidant enzyme activity responses in active and non-active individuals exposed to cigarette smoke. The study included 40 subjects who were equally classified as: smokers that did exercise (SE), smokers that did not do exercise (SnE), non-smokers that did exercise (NSE), and non-smokers that did not do exercise (NSnE). The adjusted Astrand test was used to exhaust the subjects. Salivary enzymes of peroxidase (POX), catalase (CAT), and superoxide dismutase (SOD) were measured, by spectrophotometry methods, at 3 different time points: pre-test (TP1), post-test (TP2), and one hour after finishing the test (TP3). Significant (p < 0.05) group x time interactions were found for the three enzymes. Salivary POX, CAT and SOD increased in all groups from TP1 to TP2 and decreased from TP2 to TP3. Only the NSE showed a significant difference between TP1 to TP3 in POX and SOD by +0.011 ± 0.007 and +0.075 ± 0.020 (U/mL), respectively. The NSE showed significantly higher activity of POX, CAT and SOD in TP2 compared to the other groups. Furthermore, NSE and NSnE had higher activity of POX, CAT and SOD in TP1 and TP3 (p < 0.05) compared with SE and SnE. Only in the NSnE, were no differences observed in CAT compared with SE and SnE in TP3. These results showed that the antioxidant activity at rest and in the recovery time after the acute intense exercise was lower in SE and SnE compared with NSE and NSnE, suggesting that smoking habit may reduce the ameliorating effect of regular physical activity on acute exercise-induced oxidative stress. MDPI 2021-01-27 /pmc/articles/PMC7910903/ /pubmed/33513978 http://dx.doi.org/10.3390/biom11020171 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nobari, Hadi
Nejad, Hamzeh Abdi
Kargarfard, Mehdi
Mohseni, Soghra
Suzuki, Katsuhiko
Carmelo Adsuar, José
Pérez-Gómez, Jorge
The Effect of Acute Intense Exercise on Activity of Antioxidant Enzymes in Smokers and Non-Smokers
title The Effect of Acute Intense Exercise on Activity of Antioxidant Enzymes in Smokers and Non-Smokers
title_full The Effect of Acute Intense Exercise on Activity of Antioxidant Enzymes in Smokers and Non-Smokers
title_fullStr The Effect of Acute Intense Exercise on Activity of Antioxidant Enzymes in Smokers and Non-Smokers
title_full_unstemmed The Effect of Acute Intense Exercise on Activity of Antioxidant Enzymes in Smokers and Non-Smokers
title_short The Effect of Acute Intense Exercise on Activity of Antioxidant Enzymes in Smokers and Non-Smokers
title_sort effect of acute intense exercise on activity of antioxidant enzymes in smokers and non-smokers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910903/
https://www.ncbi.nlm.nih.gov/pubmed/33513978
http://dx.doi.org/10.3390/biom11020171
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