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Serological Survey and Molecular Characterization of Theileria annulata in Sicilian Cattle

Tropical theileriosis is a tick-borne disease caused by hemoprotozoan parasites with considerable veterinary and economic impact worldwide. Ticks transmitting the disease belong to the Haemaphysalis, Rhipicephalus, and Hyalomma genera. The Hyalomma genus is very common in Sicily (Italy) and represen...

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Autores principales: Gargano, Valeria, Blanda, Valeria, Gambino, Delia, La Russa, Francesco, Di Cataldo, Sophia, Gentile, Antonino, Schirò, Giorgia, Torina, Alessandra, Millán, Javier, Vicari, Domenico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910905/
https://www.ncbi.nlm.nih.gov/pubmed/33494377
http://dx.doi.org/10.3390/pathogens10020101
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author Gargano, Valeria
Blanda, Valeria
Gambino, Delia
La Russa, Francesco
Di Cataldo, Sophia
Gentile, Antonino
Schirò, Giorgia
Torina, Alessandra
Millán, Javier
Vicari, Domenico
author_facet Gargano, Valeria
Blanda, Valeria
Gambino, Delia
La Russa, Francesco
Di Cataldo, Sophia
Gentile, Antonino
Schirò, Giorgia
Torina, Alessandra
Millán, Javier
Vicari, Domenico
author_sort Gargano, Valeria
collection PubMed
description Tropical theileriosis is a tick-borne disease caused by hemoprotozoan parasites with considerable veterinary and economic impact worldwide. Ticks transmitting the disease belong to the Haemaphysalis, Rhipicephalus, and Hyalomma genera. The Hyalomma genus is very common in Sicily (Italy) and represents the main Theileria annulata vector in the island. Data concerning the molecular epidemiology of this pathogen are missing in the region. In 2018–2019, blood and serum samples were collected from 480 cows in seven Sicilian farms from four different provinces. Seroprevalence in the farms ranged from 22% to 71%. Three farms were selected for molecular analysis consisting of real-time PCR targeting the almost complete 18S ribosomal RNA (rRNA). Four amplicons per farm were sequenced and phylogenetic analyses were carried out. The four sequences were identical within each farm and showed 92–99% identity with the other farms and with sequences from Genbank. According to the phylogenetic analysis, these three sequences and an additional one from a laboratory-cultured Theileria annulata strain obtained in 1999 belonged to a single T. annulata clade with good bootstrap support with other sequences from Italy, India, and Iran, indicating limited geographical and temporal genetic variability of the parasite. This study represents the first phylogenetic analysis of T. annulata in Sicily, which will be useful to improve the strategies for theileriosis control and prevention.
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spelling pubmed-79109052021-02-28 Serological Survey and Molecular Characterization of Theileria annulata in Sicilian Cattle Gargano, Valeria Blanda, Valeria Gambino, Delia La Russa, Francesco Di Cataldo, Sophia Gentile, Antonino Schirò, Giorgia Torina, Alessandra Millán, Javier Vicari, Domenico Pathogens Communication Tropical theileriosis is a tick-borne disease caused by hemoprotozoan parasites with considerable veterinary and economic impact worldwide. Ticks transmitting the disease belong to the Haemaphysalis, Rhipicephalus, and Hyalomma genera. The Hyalomma genus is very common in Sicily (Italy) and represents the main Theileria annulata vector in the island. Data concerning the molecular epidemiology of this pathogen are missing in the region. In 2018–2019, blood and serum samples were collected from 480 cows in seven Sicilian farms from four different provinces. Seroprevalence in the farms ranged from 22% to 71%. Three farms were selected for molecular analysis consisting of real-time PCR targeting the almost complete 18S ribosomal RNA (rRNA). Four amplicons per farm were sequenced and phylogenetic analyses were carried out. The four sequences were identical within each farm and showed 92–99% identity with the other farms and with sequences from Genbank. According to the phylogenetic analysis, these three sequences and an additional one from a laboratory-cultured Theileria annulata strain obtained in 1999 belonged to a single T. annulata clade with good bootstrap support with other sequences from Italy, India, and Iran, indicating limited geographical and temporal genetic variability of the parasite. This study represents the first phylogenetic analysis of T. annulata in Sicily, which will be useful to improve the strategies for theileriosis control and prevention. MDPI 2021-01-21 /pmc/articles/PMC7910905/ /pubmed/33494377 http://dx.doi.org/10.3390/pathogens10020101 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Gargano, Valeria
Blanda, Valeria
Gambino, Delia
La Russa, Francesco
Di Cataldo, Sophia
Gentile, Antonino
Schirò, Giorgia
Torina, Alessandra
Millán, Javier
Vicari, Domenico
Serological Survey and Molecular Characterization of Theileria annulata in Sicilian Cattle
title Serological Survey and Molecular Characterization of Theileria annulata in Sicilian Cattle
title_full Serological Survey and Molecular Characterization of Theileria annulata in Sicilian Cattle
title_fullStr Serological Survey and Molecular Characterization of Theileria annulata in Sicilian Cattle
title_full_unstemmed Serological Survey and Molecular Characterization of Theileria annulata in Sicilian Cattle
title_short Serological Survey and Molecular Characterization of Theileria annulata in Sicilian Cattle
title_sort serological survey and molecular characterization of theileria annulata in sicilian cattle
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910905/
https://www.ncbi.nlm.nih.gov/pubmed/33494377
http://dx.doi.org/10.3390/pathogens10020101
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