Cargando…
The Capsid Protein of Rubella Virus Antagonizes RNA Interference in Mammalian Cells
Rubella virus (RuV) is the infectious agent of a series of birth defect diseases termed congenital rubella syndrome, which is a major public health concern all around the world. RNA interference (RNAi) is a crucial antiviral defense mechanism in eukaryotes, and numerous viruses have been found to en...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910915/ https://www.ncbi.nlm.nih.gov/pubmed/33494454 http://dx.doi.org/10.3390/v13020154 |
_version_ | 1783656223741050880 |
---|---|
author | Xu, Jiuyue Kong, Jing Lyu, Bao Wang, Xiaotong Qian, Qi Zhou, Xi Qiu, Yang |
author_facet | Xu, Jiuyue Kong, Jing Lyu, Bao Wang, Xiaotong Qian, Qi Zhou, Xi Qiu, Yang |
author_sort | Xu, Jiuyue |
collection | PubMed |
description | Rubella virus (RuV) is the infectious agent of a series of birth defect diseases termed congenital rubella syndrome, which is a major public health concern all around the world. RNA interference (RNAi) is a crucial antiviral defense mechanism in eukaryotes, and numerous viruses have been found to encode viral suppressors of RNAi (VSRs) to evade antiviral RNAi response. However, there is little knowledge about whether and how RuV antagonizes RNAi. In this study, we identified that the RuV capsid protein is a potent VSR that can efficiently suppress shRNA- and siRNA-induced RNAi in mammalian cells. Moreover, the VSR activity of the RuV capsid is dependent on its dimerization and double-stranded RNA (dsRNA)-binding activity. In addition, ectopic expression of the RuV capsid can effectively rescue the replication defect of a VSR-deficient virus or replicon, implying that the RuV capsid can act as a VSR in the context of viral infection. Together, our findings uncover that RuV encodes a VSR to evade antiviral RNAi response, which expands our understanding of RuV–host interaction and sheds light on the potential therapeutic target against RuV. |
format | Online Article Text |
id | pubmed-7910915 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79109152021-02-28 The Capsid Protein of Rubella Virus Antagonizes RNA Interference in Mammalian Cells Xu, Jiuyue Kong, Jing Lyu, Bao Wang, Xiaotong Qian, Qi Zhou, Xi Qiu, Yang Viruses Article Rubella virus (RuV) is the infectious agent of a series of birth defect diseases termed congenital rubella syndrome, which is a major public health concern all around the world. RNA interference (RNAi) is a crucial antiviral defense mechanism in eukaryotes, and numerous viruses have been found to encode viral suppressors of RNAi (VSRs) to evade antiviral RNAi response. However, there is little knowledge about whether and how RuV antagonizes RNAi. In this study, we identified that the RuV capsid protein is a potent VSR that can efficiently suppress shRNA- and siRNA-induced RNAi in mammalian cells. Moreover, the VSR activity of the RuV capsid is dependent on its dimerization and double-stranded RNA (dsRNA)-binding activity. In addition, ectopic expression of the RuV capsid can effectively rescue the replication defect of a VSR-deficient virus or replicon, implying that the RuV capsid can act as a VSR in the context of viral infection. Together, our findings uncover that RuV encodes a VSR to evade antiviral RNAi response, which expands our understanding of RuV–host interaction and sheds light on the potential therapeutic target against RuV. MDPI 2021-01-21 /pmc/articles/PMC7910915/ /pubmed/33494454 http://dx.doi.org/10.3390/v13020154 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xu, Jiuyue Kong, Jing Lyu, Bao Wang, Xiaotong Qian, Qi Zhou, Xi Qiu, Yang The Capsid Protein of Rubella Virus Antagonizes RNA Interference in Mammalian Cells |
title | The Capsid Protein of Rubella Virus Antagonizes RNA Interference in Mammalian Cells |
title_full | The Capsid Protein of Rubella Virus Antagonizes RNA Interference in Mammalian Cells |
title_fullStr | The Capsid Protein of Rubella Virus Antagonizes RNA Interference in Mammalian Cells |
title_full_unstemmed | The Capsid Protein of Rubella Virus Antagonizes RNA Interference in Mammalian Cells |
title_short | The Capsid Protein of Rubella Virus Antagonizes RNA Interference in Mammalian Cells |
title_sort | capsid protein of rubella virus antagonizes rna interference in mammalian cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910915/ https://www.ncbi.nlm.nih.gov/pubmed/33494454 http://dx.doi.org/10.3390/v13020154 |
work_keys_str_mv | AT xujiuyue thecapsidproteinofrubellavirusantagonizesrnainterferenceinmammaliancells AT kongjing thecapsidproteinofrubellavirusantagonizesrnainterferenceinmammaliancells AT lyubao thecapsidproteinofrubellavirusantagonizesrnainterferenceinmammaliancells AT wangxiaotong thecapsidproteinofrubellavirusantagonizesrnainterferenceinmammaliancells AT qianqi thecapsidproteinofrubellavirusantagonizesrnainterferenceinmammaliancells AT zhouxi thecapsidproteinofrubellavirusantagonizesrnainterferenceinmammaliancells AT qiuyang thecapsidproteinofrubellavirusantagonizesrnainterferenceinmammaliancells AT xujiuyue capsidproteinofrubellavirusantagonizesrnainterferenceinmammaliancells AT kongjing capsidproteinofrubellavirusantagonizesrnainterferenceinmammaliancells AT lyubao capsidproteinofrubellavirusantagonizesrnainterferenceinmammaliancells AT wangxiaotong capsidproteinofrubellavirusantagonizesrnainterferenceinmammaliancells AT qianqi capsidproteinofrubellavirusantagonizesrnainterferenceinmammaliancells AT zhouxi capsidproteinofrubellavirusantagonizesrnainterferenceinmammaliancells AT qiuyang capsidproteinofrubellavirusantagonizesrnainterferenceinmammaliancells |