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MPC Polymer Promotes Recovery from Dry Eye via Stabilization of the Ocular Surface
The polymer that includes 2-methacryloyloxy ethyl phosphorylcholine (MPC) is well-known as an effectively hydrating multifunction agent. In this study, we prepared an MPC polymer (MPCP) using radical polymerization with co-monomers—MPC/Stearyl Methacrylate/N,N-dimethylacrylamide—and evaluated the MP...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910948/ https://www.ncbi.nlm.nih.gov/pubmed/33513827 http://dx.doi.org/10.3390/pharmaceutics13020168 |
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author | Nagai, Noriaki Sakurai, Shunsuke Seiriki, Ryotaro Minami, Misa Yamaguchi, Mizuki Deguchi, Saori Harata, Eiji |
author_facet | Nagai, Noriaki Sakurai, Shunsuke Seiriki, Ryotaro Minami, Misa Yamaguchi, Mizuki Deguchi, Saori Harata, Eiji |
author_sort | Nagai, Noriaki |
collection | PubMed |
description | The polymer that includes 2-methacryloyloxy ethyl phosphorylcholine (MPC) is well-known as an effectively hydrating multifunction agent. In this study, we prepared an MPC polymer (MPCP) using radical polymerization with co-monomers—MPC/Stearyl Methacrylate/N,N-dimethylacrylamide—and evaluated the MPCP’s usefulness for dry eye treatment using a rabbit model treated with N-acetylcysteine. The MPCP particle size was 50–250 nm, and the form was similar to that of micelles. The MPCP viscosity (approximately 0.95 mPa·s) was 1.17-fold that of purified water, and a decrease in the transepithelial electrical resistance value (corneal damage) was not observed in the immortalized human corneal epithelial cell line HCE-T cell (HCE-T cell layer). The MPCP enhanced the water maintenance on the cornea, and the instillation of MPCP increased the lacrimal fluid volume and prolonged the tear film breakup time without an increase in total mucin contents in the lacrimal fluid of the normal rabbits. The therapeutic potential of the MPCP for dry eye was evaluated using an N-acetylcysteine-treated rabbit model, and, in our investigation, we found that MPCP enhanced the volume of lacrimal fluid and promoted an improvement in the tear film breakup levels. These findings regarding the creation and characteristics of a novel MPCP will provide relevant information for designing further studies to develop a treatment for dry eyes. |
format | Online Article Text |
id | pubmed-7910948 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79109482021-02-28 MPC Polymer Promotes Recovery from Dry Eye via Stabilization of the Ocular Surface Nagai, Noriaki Sakurai, Shunsuke Seiriki, Ryotaro Minami, Misa Yamaguchi, Mizuki Deguchi, Saori Harata, Eiji Pharmaceutics Article The polymer that includes 2-methacryloyloxy ethyl phosphorylcholine (MPC) is well-known as an effectively hydrating multifunction agent. In this study, we prepared an MPC polymer (MPCP) using radical polymerization with co-monomers—MPC/Stearyl Methacrylate/N,N-dimethylacrylamide—and evaluated the MPCP’s usefulness for dry eye treatment using a rabbit model treated with N-acetylcysteine. The MPCP particle size was 50–250 nm, and the form was similar to that of micelles. The MPCP viscosity (approximately 0.95 mPa·s) was 1.17-fold that of purified water, and a decrease in the transepithelial electrical resistance value (corneal damage) was not observed in the immortalized human corneal epithelial cell line HCE-T cell (HCE-T cell layer). The MPCP enhanced the water maintenance on the cornea, and the instillation of MPCP increased the lacrimal fluid volume and prolonged the tear film breakup time without an increase in total mucin contents in the lacrimal fluid of the normal rabbits. The therapeutic potential of the MPCP for dry eye was evaluated using an N-acetylcysteine-treated rabbit model, and, in our investigation, we found that MPCP enhanced the volume of lacrimal fluid and promoted an improvement in the tear film breakup levels. These findings regarding the creation and characteristics of a novel MPCP will provide relevant information for designing further studies to develop a treatment for dry eyes. MDPI 2021-01-27 /pmc/articles/PMC7910948/ /pubmed/33513827 http://dx.doi.org/10.3390/pharmaceutics13020168 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nagai, Noriaki Sakurai, Shunsuke Seiriki, Ryotaro Minami, Misa Yamaguchi, Mizuki Deguchi, Saori Harata, Eiji MPC Polymer Promotes Recovery from Dry Eye via Stabilization of the Ocular Surface |
title | MPC Polymer Promotes Recovery from Dry Eye via Stabilization of the Ocular Surface |
title_full | MPC Polymer Promotes Recovery from Dry Eye via Stabilization of the Ocular Surface |
title_fullStr | MPC Polymer Promotes Recovery from Dry Eye via Stabilization of the Ocular Surface |
title_full_unstemmed | MPC Polymer Promotes Recovery from Dry Eye via Stabilization of the Ocular Surface |
title_short | MPC Polymer Promotes Recovery from Dry Eye via Stabilization of the Ocular Surface |
title_sort | mpc polymer promotes recovery from dry eye via stabilization of the ocular surface |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910948/ https://www.ncbi.nlm.nih.gov/pubmed/33513827 http://dx.doi.org/10.3390/pharmaceutics13020168 |
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