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MPC Polymer Promotes Recovery from Dry Eye via Stabilization of the Ocular Surface

The polymer that includes 2-methacryloyloxy ethyl phosphorylcholine (MPC) is well-known as an effectively hydrating multifunction agent. In this study, we prepared an MPC polymer (MPCP) using radical polymerization with co-monomers—MPC/Stearyl Methacrylate/N,N-dimethylacrylamide—and evaluated the MP...

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Autores principales: Nagai, Noriaki, Sakurai, Shunsuke, Seiriki, Ryotaro, Minami, Misa, Yamaguchi, Mizuki, Deguchi, Saori, Harata, Eiji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910948/
https://www.ncbi.nlm.nih.gov/pubmed/33513827
http://dx.doi.org/10.3390/pharmaceutics13020168
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author Nagai, Noriaki
Sakurai, Shunsuke
Seiriki, Ryotaro
Minami, Misa
Yamaguchi, Mizuki
Deguchi, Saori
Harata, Eiji
author_facet Nagai, Noriaki
Sakurai, Shunsuke
Seiriki, Ryotaro
Minami, Misa
Yamaguchi, Mizuki
Deguchi, Saori
Harata, Eiji
author_sort Nagai, Noriaki
collection PubMed
description The polymer that includes 2-methacryloyloxy ethyl phosphorylcholine (MPC) is well-known as an effectively hydrating multifunction agent. In this study, we prepared an MPC polymer (MPCP) using radical polymerization with co-monomers—MPC/Stearyl Methacrylate/N,N-dimethylacrylamide—and evaluated the MPCP’s usefulness for dry eye treatment using a rabbit model treated with N-acetylcysteine. The MPCP particle size was 50–250 nm, and the form was similar to that of micelles. The MPCP viscosity (approximately 0.95 mPa·s) was 1.17-fold that of purified water, and a decrease in the transepithelial electrical resistance value (corneal damage) was not observed in the immortalized human corneal epithelial cell line HCE-T cell (HCE-T cell layer). The MPCP enhanced the water maintenance on the cornea, and the instillation of MPCP increased the lacrimal fluid volume and prolonged the tear film breakup time without an increase in total mucin contents in the lacrimal fluid of the normal rabbits. The therapeutic potential of the MPCP for dry eye was evaluated using an N-acetylcysteine-treated rabbit model, and, in our investigation, we found that MPCP enhanced the volume of lacrimal fluid and promoted an improvement in the tear film breakup levels. These findings regarding the creation and characteristics of a novel MPCP will provide relevant information for designing further studies to develop a treatment for dry eyes.
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spelling pubmed-79109482021-02-28 MPC Polymer Promotes Recovery from Dry Eye via Stabilization of the Ocular Surface Nagai, Noriaki Sakurai, Shunsuke Seiriki, Ryotaro Minami, Misa Yamaguchi, Mizuki Deguchi, Saori Harata, Eiji Pharmaceutics Article The polymer that includes 2-methacryloyloxy ethyl phosphorylcholine (MPC) is well-known as an effectively hydrating multifunction agent. In this study, we prepared an MPC polymer (MPCP) using radical polymerization with co-monomers—MPC/Stearyl Methacrylate/N,N-dimethylacrylamide—and evaluated the MPCP’s usefulness for dry eye treatment using a rabbit model treated with N-acetylcysteine. The MPCP particle size was 50–250 nm, and the form was similar to that of micelles. The MPCP viscosity (approximately 0.95 mPa·s) was 1.17-fold that of purified water, and a decrease in the transepithelial electrical resistance value (corneal damage) was not observed in the immortalized human corneal epithelial cell line HCE-T cell (HCE-T cell layer). The MPCP enhanced the water maintenance on the cornea, and the instillation of MPCP increased the lacrimal fluid volume and prolonged the tear film breakup time without an increase in total mucin contents in the lacrimal fluid of the normal rabbits. The therapeutic potential of the MPCP for dry eye was evaluated using an N-acetylcysteine-treated rabbit model, and, in our investigation, we found that MPCP enhanced the volume of lacrimal fluid and promoted an improvement in the tear film breakup levels. These findings regarding the creation and characteristics of a novel MPCP will provide relevant information for designing further studies to develop a treatment for dry eyes. MDPI 2021-01-27 /pmc/articles/PMC7910948/ /pubmed/33513827 http://dx.doi.org/10.3390/pharmaceutics13020168 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nagai, Noriaki
Sakurai, Shunsuke
Seiriki, Ryotaro
Minami, Misa
Yamaguchi, Mizuki
Deguchi, Saori
Harata, Eiji
MPC Polymer Promotes Recovery from Dry Eye via Stabilization of the Ocular Surface
title MPC Polymer Promotes Recovery from Dry Eye via Stabilization of the Ocular Surface
title_full MPC Polymer Promotes Recovery from Dry Eye via Stabilization of the Ocular Surface
title_fullStr MPC Polymer Promotes Recovery from Dry Eye via Stabilization of the Ocular Surface
title_full_unstemmed MPC Polymer Promotes Recovery from Dry Eye via Stabilization of the Ocular Surface
title_short MPC Polymer Promotes Recovery from Dry Eye via Stabilization of the Ocular Surface
title_sort mpc polymer promotes recovery from dry eye via stabilization of the ocular surface
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910948/
https://www.ncbi.nlm.nih.gov/pubmed/33513827
http://dx.doi.org/10.3390/pharmaceutics13020168
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