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Chronic Oleoylethanolamide Treatment Decreases Hepatic Triacylglycerol Level in Rat Liver by a PPARγ/SREBP-Mediated Suppression of Fatty Acid and Triacylglycerol Synthesis

Oleoylethanolamide (OEA) is a naturally occurring bioactive lipid belonging to the family of N-acylethanolamides. A variety of beneficial effects have been attributed to OEA, although the greater interest is due to its potential role in the treatment of obesity, fatty liver, and eating-related disor...

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Autores principales: Romano, Adele, Friuli, Marzia, Del Coco, Laura, Longo, Serena, Vergara, Daniele, Del Boccio, Piero, Valentinuzzi, Silvia, Cicalini, Ilaria, Fanizzi, Francesco P., Gaetani, Silvana, Giudetti, Anna M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910994/
https://www.ncbi.nlm.nih.gov/pubmed/33513874
http://dx.doi.org/10.3390/nu13020394
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author Romano, Adele
Friuli, Marzia
Del Coco, Laura
Longo, Serena
Vergara, Daniele
Del Boccio, Piero
Valentinuzzi, Silvia
Cicalini, Ilaria
Fanizzi, Francesco P.
Gaetani, Silvana
Giudetti, Anna M.
author_facet Romano, Adele
Friuli, Marzia
Del Coco, Laura
Longo, Serena
Vergara, Daniele
Del Boccio, Piero
Valentinuzzi, Silvia
Cicalini, Ilaria
Fanizzi, Francesco P.
Gaetani, Silvana
Giudetti, Anna M.
author_sort Romano, Adele
collection PubMed
description Oleoylethanolamide (OEA) is a naturally occurring bioactive lipid belonging to the family of N-acylethanolamides. A variety of beneficial effects have been attributed to OEA, although the greater interest is due to its potential role in the treatment of obesity, fatty liver, and eating-related disorders. To better clarify the mechanism of the antiadipogenic effect of OEA in the liver, using a lipidomic study performed by (1)H-NMR, LC-MS/MS and thin-layer chromatography analyses we evaluated the whole lipid composition of rat liver, following a two-week daily treatment of OEA (10 mg kg(−1) i.p.). We found that OEA induced a significant reduction in hepatic triacylglycerol (TAG) content and significant changes in sphingolipid composition and ceramidase activity. We associated the antiadipogenic effect of OEA to decreased activity and expression of key enzymes involved in fatty acid and TAG syntheses, such as acetyl-CoA carboxylase, fatty acid synthase, diacylglycerol acyltransferase, and stearoyl-CoA desaturase 1. Moreover, we found that both SREBP-1 and PPARγ protein expression were significantly reduced in the liver of OEA-treated rats. Our findings add significant and important insights into the molecular mechanism of OEA on hepatic adipogenesis, and suggest a possible link between the OEA-induced changes in sphingolipid metabolism and suppression of hepatic TAG level.
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spelling pubmed-79109942021-02-28 Chronic Oleoylethanolamide Treatment Decreases Hepatic Triacylglycerol Level in Rat Liver by a PPARγ/SREBP-Mediated Suppression of Fatty Acid and Triacylglycerol Synthesis Romano, Adele Friuli, Marzia Del Coco, Laura Longo, Serena Vergara, Daniele Del Boccio, Piero Valentinuzzi, Silvia Cicalini, Ilaria Fanizzi, Francesco P. Gaetani, Silvana Giudetti, Anna M. Nutrients Article Oleoylethanolamide (OEA) is a naturally occurring bioactive lipid belonging to the family of N-acylethanolamides. A variety of beneficial effects have been attributed to OEA, although the greater interest is due to its potential role in the treatment of obesity, fatty liver, and eating-related disorders. To better clarify the mechanism of the antiadipogenic effect of OEA in the liver, using a lipidomic study performed by (1)H-NMR, LC-MS/MS and thin-layer chromatography analyses we evaluated the whole lipid composition of rat liver, following a two-week daily treatment of OEA (10 mg kg(−1) i.p.). We found that OEA induced a significant reduction in hepatic triacylglycerol (TAG) content and significant changes in sphingolipid composition and ceramidase activity. We associated the antiadipogenic effect of OEA to decreased activity and expression of key enzymes involved in fatty acid and TAG syntheses, such as acetyl-CoA carboxylase, fatty acid synthase, diacylglycerol acyltransferase, and stearoyl-CoA desaturase 1. Moreover, we found that both SREBP-1 and PPARγ protein expression were significantly reduced in the liver of OEA-treated rats. Our findings add significant and important insights into the molecular mechanism of OEA on hepatic adipogenesis, and suggest a possible link between the OEA-induced changes in sphingolipid metabolism and suppression of hepatic TAG level. MDPI 2021-01-27 /pmc/articles/PMC7910994/ /pubmed/33513874 http://dx.doi.org/10.3390/nu13020394 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Romano, Adele
Friuli, Marzia
Del Coco, Laura
Longo, Serena
Vergara, Daniele
Del Boccio, Piero
Valentinuzzi, Silvia
Cicalini, Ilaria
Fanizzi, Francesco P.
Gaetani, Silvana
Giudetti, Anna M.
Chronic Oleoylethanolamide Treatment Decreases Hepatic Triacylglycerol Level in Rat Liver by a PPARγ/SREBP-Mediated Suppression of Fatty Acid and Triacylglycerol Synthesis
title Chronic Oleoylethanolamide Treatment Decreases Hepatic Triacylglycerol Level in Rat Liver by a PPARγ/SREBP-Mediated Suppression of Fatty Acid and Triacylglycerol Synthesis
title_full Chronic Oleoylethanolamide Treatment Decreases Hepatic Triacylglycerol Level in Rat Liver by a PPARγ/SREBP-Mediated Suppression of Fatty Acid and Triacylglycerol Synthesis
title_fullStr Chronic Oleoylethanolamide Treatment Decreases Hepatic Triacylglycerol Level in Rat Liver by a PPARγ/SREBP-Mediated Suppression of Fatty Acid and Triacylglycerol Synthesis
title_full_unstemmed Chronic Oleoylethanolamide Treatment Decreases Hepatic Triacylglycerol Level in Rat Liver by a PPARγ/SREBP-Mediated Suppression of Fatty Acid and Triacylglycerol Synthesis
title_short Chronic Oleoylethanolamide Treatment Decreases Hepatic Triacylglycerol Level in Rat Liver by a PPARγ/SREBP-Mediated Suppression of Fatty Acid and Triacylglycerol Synthesis
title_sort chronic oleoylethanolamide treatment decreases hepatic triacylglycerol level in rat liver by a pparγ/srebp-mediated suppression of fatty acid and triacylglycerol synthesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910994/
https://www.ncbi.nlm.nih.gov/pubmed/33513874
http://dx.doi.org/10.3390/nu13020394
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