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Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation

Fertility preservation for male childhood cancer survivors not yet capable of producing mature spermatozoa, relies on experimental approaches such as testicular explant culture. Although the first steps in somatic maturation can be observed in human testicular explant cultures, germ cell depletion i...

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Autores principales: Kurek, Magdalena, Åkesson, Elisabet, Yoshihara, Masahito, Oliver, Elizabeth, Cui, Yanhua, Becker, Martin, Alves-Lopes, João Pedro, Bjarnason, Ragnar, Romerius, Patrik, Sundin, Mikael, Norén Nyström, Ulrika, Langenskiöld, Cecilia, Vogt, Hartmut, Henningsohn, Lars, Petersen, Cecilia, Söder, Olle, Guo, Jingtao, Mitchell, Rod T., Jahnukainen, Kirsi, Stukenborg, Jan-Bernd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911157/
https://www.ncbi.nlm.nih.gov/pubmed/33513766
http://dx.doi.org/10.3390/cells10020241
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author Kurek, Magdalena
Åkesson, Elisabet
Yoshihara, Masahito
Oliver, Elizabeth
Cui, Yanhua
Becker, Martin
Alves-Lopes, João Pedro
Bjarnason, Ragnar
Romerius, Patrik
Sundin, Mikael
Norén Nyström, Ulrika
Langenskiöld, Cecilia
Vogt, Hartmut
Henningsohn, Lars
Petersen, Cecilia
Söder, Olle
Guo, Jingtao
Mitchell, Rod T.
Jahnukainen, Kirsi
Stukenborg, Jan-Bernd
author_facet Kurek, Magdalena
Åkesson, Elisabet
Yoshihara, Masahito
Oliver, Elizabeth
Cui, Yanhua
Becker, Martin
Alves-Lopes, João Pedro
Bjarnason, Ragnar
Romerius, Patrik
Sundin, Mikael
Norén Nyström, Ulrika
Langenskiöld, Cecilia
Vogt, Hartmut
Henningsohn, Lars
Petersen, Cecilia
Söder, Olle
Guo, Jingtao
Mitchell, Rod T.
Jahnukainen, Kirsi
Stukenborg, Jan-Bernd
author_sort Kurek, Magdalena
collection PubMed
description Fertility preservation for male childhood cancer survivors not yet capable of producing mature spermatozoa, relies on experimental approaches such as testicular explant culture. Although the first steps in somatic maturation can be observed in human testicular explant cultures, germ cell depletion is a common obstacle. Hence, understanding the spermatogonial stem cell (SSC) niche environment and in particular, specific components such as the seminiferous basement membrane (BM) will allow progression of testicular explant cultures. Here, we revealed that the seminiferous BM is established from 6 weeks post conception with the expression of laminin alpha 1 (LAMA 1) and type IV collagen, which persist as key components throughout development. With prepubertal testicular explant culture we found that seminiferous LAMA 1 expression is disrupted and depleted with culture time correlating with germ cell loss. These findings highlight the importance of LAMA 1 for the human SSC niche and its sensitivity to culture conditions.
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spelling pubmed-79111572021-02-28 Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation Kurek, Magdalena Åkesson, Elisabet Yoshihara, Masahito Oliver, Elizabeth Cui, Yanhua Becker, Martin Alves-Lopes, João Pedro Bjarnason, Ragnar Romerius, Patrik Sundin, Mikael Norén Nyström, Ulrika Langenskiöld, Cecilia Vogt, Hartmut Henningsohn, Lars Petersen, Cecilia Söder, Olle Guo, Jingtao Mitchell, Rod T. Jahnukainen, Kirsi Stukenborg, Jan-Bernd Cells Article Fertility preservation for male childhood cancer survivors not yet capable of producing mature spermatozoa, relies on experimental approaches such as testicular explant culture. Although the first steps in somatic maturation can be observed in human testicular explant cultures, germ cell depletion is a common obstacle. Hence, understanding the spermatogonial stem cell (SSC) niche environment and in particular, specific components such as the seminiferous basement membrane (BM) will allow progression of testicular explant cultures. Here, we revealed that the seminiferous BM is established from 6 weeks post conception with the expression of laminin alpha 1 (LAMA 1) and type IV collagen, which persist as key components throughout development. With prepubertal testicular explant culture we found that seminiferous LAMA 1 expression is disrupted and depleted with culture time correlating with germ cell loss. These findings highlight the importance of LAMA 1 for the human SSC niche and its sensitivity to culture conditions. MDPI 2021-01-27 /pmc/articles/PMC7911157/ /pubmed/33513766 http://dx.doi.org/10.3390/cells10020241 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kurek, Magdalena
Åkesson, Elisabet
Yoshihara, Masahito
Oliver, Elizabeth
Cui, Yanhua
Becker, Martin
Alves-Lopes, João Pedro
Bjarnason, Ragnar
Romerius, Patrik
Sundin, Mikael
Norén Nyström, Ulrika
Langenskiöld, Cecilia
Vogt, Hartmut
Henningsohn, Lars
Petersen, Cecilia
Söder, Olle
Guo, Jingtao
Mitchell, Rod T.
Jahnukainen, Kirsi
Stukenborg, Jan-Bernd
Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation
title Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation
title_full Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation
title_fullStr Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation
title_full_unstemmed Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation
title_short Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation
title_sort spermatogonia loss correlates with lama 1 expression in human prepubertal testes stored for fertility preservation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911157/
https://www.ncbi.nlm.nih.gov/pubmed/33513766
http://dx.doi.org/10.3390/cells10020241
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