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Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation
Fertility preservation for male childhood cancer survivors not yet capable of producing mature spermatozoa, relies on experimental approaches such as testicular explant culture. Although the first steps in somatic maturation can be observed in human testicular explant cultures, germ cell depletion i...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911157/ https://www.ncbi.nlm.nih.gov/pubmed/33513766 http://dx.doi.org/10.3390/cells10020241 |
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author | Kurek, Magdalena Åkesson, Elisabet Yoshihara, Masahito Oliver, Elizabeth Cui, Yanhua Becker, Martin Alves-Lopes, João Pedro Bjarnason, Ragnar Romerius, Patrik Sundin, Mikael Norén Nyström, Ulrika Langenskiöld, Cecilia Vogt, Hartmut Henningsohn, Lars Petersen, Cecilia Söder, Olle Guo, Jingtao Mitchell, Rod T. Jahnukainen, Kirsi Stukenborg, Jan-Bernd |
author_facet | Kurek, Magdalena Åkesson, Elisabet Yoshihara, Masahito Oliver, Elizabeth Cui, Yanhua Becker, Martin Alves-Lopes, João Pedro Bjarnason, Ragnar Romerius, Patrik Sundin, Mikael Norén Nyström, Ulrika Langenskiöld, Cecilia Vogt, Hartmut Henningsohn, Lars Petersen, Cecilia Söder, Olle Guo, Jingtao Mitchell, Rod T. Jahnukainen, Kirsi Stukenborg, Jan-Bernd |
author_sort | Kurek, Magdalena |
collection | PubMed |
description | Fertility preservation for male childhood cancer survivors not yet capable of producing mature spermatozoa, relies on experimental approaches such as testicular explant culture. Although the first steps in somatic maturation can be observed in human testicular explant cultures, germ cell depletion is a common obstacle. Hence, understanding the spermatogonial stem cell (SSC) niche environment and in particular, specific components such as the seminiferous basement membrane (BM) will allow progression of testicular explant cultures. Here, we revealed that the seminiferous BM is established from 6 weeks post conception with the expression of laminin alpha 1 (LAMA 1) and type IV collagen, which persist as key components throughout development. With prepubertal testicular explant culture we found that seminiferous LAMA 1 expression is disrupted and depleted with culture time correlating with germ cell loss. These findings highlight the importance of LAMA 1 for the human SSC niche and its sensitivity to culture conditions. |
format | Online Article Text |
id | pubmed-7911157 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79111572021-02-28 Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation Kurek, Magdalena Åkesson, Elisabet Yoshihara, Masahito Oliver, Elizabeth Cui, Yanhua Becker, Martin Alves-Lopes, João Pedro Bjarnason, Ragnar Romerius, Patrik Sundin, Mikael Norén Nyström, Ulrika Langenskiöld, Cecilia Vogt, Hartmut Henningsohn, Lars Petersen, Cecilia Söder, Olle Guo, Jingtao Mitchell, Rod T. Jahnukainen, Kirsi Stukenborg, Jan-Bernd Cells Article Fertility preservation for male childhood cancer survivors not yet capable of producing mature spermatozoa, relies on experimental approaches such as testicular explant culture. Although the first steps in somatic maturation can be observed in human testicular explant cultures, germ cell depletion is a common obstacle. Hence, understanding the spermatogonial stem cell (SSC) niche environment and in particular, specific components such as the seminiferous basement membrane (BM) will allow progression of testicular explant cultures. Here, we revealed that the seminiferous BM is established from 6 weeks post conception with the expression of laminin alpha 1 (LAMA 1) and type IV collagen, which persist as key components throughout development. With prepubertal testicular explant culture we found that seminiferous LAMA 1 expression is disrupted and depleted with culture time correlating with germ cell loss. These findings highlight the importance of LAMA 1 for the human SSC niche and its sensitivity to culture conditions. MDPI 2021-01-27 /pmc/articles/PMC7911157/ /pubmed/33513766 http://dx.doi.org/10.3390/cells10020241 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kurek, Magdalena Åkesson, Elisabet Yoshihara, Masahito Oliver, Elizabeth Cui, Yanhua Becker, Martin Alves-Lopes, João Pedro Bjarnason, Ragnar Romerius, Patrik Sundin, Mikael Norén Nyström, Ulrika Langenskiöld, Cecilia Vogt, Hartmut Henningsohn, Lars Petersen, Cecilia Söder, Olle Guo, Jingtao Mitchell, Rod T. Jahnukainen, Kirsi Stukenborg, Jan-Bernd Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation |
title | Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation |
title_full | Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation |
title_fullStr | Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation |
title_full_unstemmed | Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation |
title_short | Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation |
title_sort | spermatogonia loss correlates with lama 1 expression in human prepubertal testes stored for fertility preservation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911157/ https://www.ncbi.nlm.nih.gov/pubmed/33513766 http://dx.doi.org/10.3390/cells10020241 |
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