Cargando…

Genomic Characterisation of a Novel Avipoxvirus Isolated from an Endangered Yellow-Eyed Penguin (Megadyptes antipodes)

Emerging viral diseases have become a significant concern due to their potential consequences for animal and environmental health. Over the past few decades, it has become clear that viruses emerging in wildlife may pose a major threat to vulnerable or endangered species. Diphtheritic stomatitis, li...

Descripción completa

Detalles Bibliográficos
Autores principales: Sarker, Subir, Athukorala, Ajani, Bowden, Timothy R., Boyle, David B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911368/
https://www.ncbi.nlm.nih.gov/pubmed/33525382
http://dx.doi.org/10.3390/v13020194
_version_ 1783656324987355136
author Sarker, Subir
Athukorala, Ajani
Bowden, Timothy R.
Boyle, David B.
author_facet Sarker, Subir
Athukorala, Ajani
Bowden, Timothy R.
Boyle, David B.
author_sort Sarker, Subir
collection PubMed
description Emerging viral diseases have become a significant concern due to their potential consequences for animal and environmental health. Over the past few decades, it has become clear that viruses emerging in wildlife may pose a major threat to vulnerable or endangered species. Diphtheritic stomatitis, likely to be caused by an avipoxvirus, has been recognised as a significant cause of mortality for the endangered yellow-eyed penguin (Megadyptes antipodes) in New Zealand. However, the avipoxvirus that infects yellow-eyed penguins has remained uncharacterised. Here, we report the complete genome of a novel avipoxvirus, penguinpox virus 2 (PEPV2), which was derived from a virus isolate obtained from a skin lesion of a yellow-eyed penguin. The PEPV2 genome is 349.8 kbp in length and contains 327 predicted genes; five of these genes were found to be unique, while a further two genes were absent compared to shearwaterpox virus 2 (SWPV2). In comparison with penguinpox virus (PEPV) isolated from an African penguin, there was a lack of conservation within the central region of the genome. Subsequent phylogenetic analyses of the PEPV2 genome positioned it within a distinct subclade comprising the recently isolated avipoxvirus genome sequences from shearwater, canary, and magpie bird species, and demonstrated a high degree of sequence similarity with SWPV2 (96.27%). This is the first reported genome sequence of PEPV2 from a yellow-eyed penguin and will help to track the evolution of avipoxvirus infections in this rare and endangered species.
format Online
Article
Text
id pubmed-7911368
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-79113682021-02-28 Genomic Characterisation of a Novel Avipoxvirus Isolated from an Endangered Yellow-Eyed Penguin (Megadyptes antipodes) Sarker, Subir Athukorala, Ajani Bowden, Timothy R. Boyle, David B. Viruses Article Emerging viral diseases have become a significant concern due to their potential consequences for animal and environmental health. Over the past few decades, it has become clear that viruses emerging in wildlife may pose a major threat to vulnerable or endangered species. Diphtheritic stomatitis, likely to be caused by an avipoxvirus, has been recognised as a significant cause of mortality for the endangered yellow-eyed penguin (Megadyptes antipodes) in New Zealand. However, the avipoxvirus that infects yellow-eyed penguins has remained uncharacterised. Here, we report the complete genome of a novel avipoxvirus, penguinpox virus 2 (PEPV2), which was derived from a virus isolate obtained from a skin lesion of a yellow-eyed penguin. The PEPV2 genome is 349.8 kbp in length and contains 327 predicted genes; five of these genes were found to be unique, while a further two genes were absent compared to shearwaterpox virus 2 (SWPV2). In comparison with penguinpox virus (PEPV) isolated from an African penguin, there was a lack of conservation within the central region of the genome. Subsequent phylogenetic analyses of the PEPV2 genome positioned it within a distinct subclade comprising the recently isolated avipoxvirus genome sequences from shearwater, canary, and magpie bird species, and demonstrated a high degree of sequence similarity with SWPV2 (96.27%). This is the first reported genome sequence of PEPV2 from a yellow-eyed penguin and will help to track the evolution of avipoxvirus infections in this rare and endangered species. MDPI 2021-01-28 /pmc/articles/PMC7911368/ /pubmed/33525382 http://dx.doi.org/10.3390/v13020194 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sarker, Subir
Athukorala, Ajani
Bowden, Timothy R.
Boyle, David B.
Genomic Characterisation of a Novel Avipoxvirus Isolated from an Endangered Yellow-Eyed Penguin (Megadyptes antipodes)
title Genomic Characterisation of a Novel Avipoxvirus Isolated from an Endangered Yellow-Eyed Penguin (Megadyptes antipodes)
title_full Genomic Characterisation of a Novel Avipoxvirus Isolated from an Endangered Yellow-Eyed Penguin (Megadyptes antipodes)
title_fullStr Genomic Characterisation of a Novel Avipoxvirus Isolated from an Endangered Yellow-Eyed Penguin (Megadyptes antipodes)
title_full_unstemmed Genomic Characterisation of a Novel Avipoxvirus Isolated from an Endangered Yellow-Eyed Penguin (Megadyptes antipodes)
title_short Genomic Characterisation of a Novel Avipoxvirus Isolated from an Endangered Yellow-Eyed Penguin (Megadyptes antipodes)
title_sort genomic characterisation of a novel avipoxvirus isolated from an endangered yellow-eyed penguin (megadyptes antipodes)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911368/
https://www.ncbi.nlm.nih.gov/pubmed/33525382
http://dx.doi.org/10.3390/v13020194
work_keys_str_mv AT sarkersubir genomiccharacterisationofanovelavipoxvirusisolatedfromanendangeredyelloweyedpenguinmegadyptesantipodes
AT athukoralaajani genomiccharacterisationofanovelavipoxvirusisolatedfromanendangeredyelloweyedpenguinmegadyptesantipodes
AT bowdentimothyr genomiccharacterisationofanovelavipoxvirusisolatedfromanendangeredyelloweyedpenguinmegadyptesantipodes
AT boyledavidb genomiccharacterisationofanovelavipoxvirusisolatedfromanendangeredyelloweyedpenguinmegadyptesantipodes