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A Candida parapsilosis Overexpression Collection Reveals Genes Required for Pathogenesis
Relative to the vast data regarding the virulence mechanisms of Candida albicans, there is limited knowledge on the emerging opportunistic human pathogen Candida parapsilosis. The aim of this study was to generate and characterize an overexpression mutant collection to identify and explore virulence...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911391/ https://www.ncbi.nlm.nih.gov/pubmed/33572958 http://dx.doi.org/10.3390/jof7020097 |
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author | Pál, Sára E. Tóth, Renáta Nosanchuk, Joshua D. Vágvölgyi, Csaba Németh, Tibor Gácser, Attila |
author_facet | Pál, Sára E. Tóth, Renáta Nosanchuk, Joshua D. Vágvölgyi, Csaba Németh, Tibor Gácser, Attila |
author_sort | Pál, Sára E. |
collection | PubMed |
description | Relative to the vast data regarding the virulence mechanisms of Candida albicans, there is limited knowledge on the emerging opportunistic human pathogen Candida parapsilosis. The aim of this study was to generate and characterize an overexpression mutant collection to identify and explore virulence factors in C. parapsilosis. With the obtained mutants, we investigated stress tolerance, morphology switch, biofilm formation, phagocytosis, and in vivo virulence in Galleria mellonella larvae and mouse models. In order to evaluate the results, we compared the data from the C. parapsilosis overexpression collection analysis to the results derived from previous deletion mutant library characterizations. Of the 37 overexpression C. parapsilosis mutants, we identified eight with altered phenotypes compared to the controls. This work is the first report to identify CPAR2_107240, CPAR2_108840, CPAR2_302400, CPAR2_406400, and CPAR2_602820 as contributors to C. parapsilosis virulence by regulating functions associated with host-pathogen interactions and biofilm formation. Our findings also confirmed the role of CPAR2_109520, CPAR2_200040, and CPAR2_500180 in pathogenesis. This study was the first attempt to use an overexpression strategy to systematically assess gene function in C. parapsilosis, and our results demonstrate that this approach is effective for such investigations. |
format | Online Article Text |
id | pubmed-7911391 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79113912021-02-28 A Candida parapsilosis Overexpression Collection Reveals Genes Required for Pathogenesis Pál, Sára E. Tóth, Renáta Nosanchuk, Joshua D. Vágvölgyi, Csaba Németh, Tibor Gácser, Attila J Fungi (Basel) Article Relative to the vast data regarding the virulence mechanisms of Candida albicans, there is limited knowledge on the emerging opportunistic human pathogen Candida parapsilosis. The aim of this study was to generate and characterize an overexpression mutant collection to identify and explore virulence factors in C. parapsilosis. With the obtained mutants, we investigated stress tolerance, morphology switch, biofilm formation, phagocytosis, and in vivo virulence in Galleria mellonella larvae and mouse models. In order to evaluate the results, we compared the data from the C. parapsilosis overexpression collection analysis to the results derived from previous deletion mutant library characterizations. Of the 37 overexpression C. parapsilosis mutants, we identified eight with altered phenotypes compared to the controls. This work is the first report to identify CPAR2_107240, CPAR2_108840, CPAR2_302400, CPAR2_406400, and CPAR2_602820 as contributors to C. parapsilosis virulence by regulating functions associated with host-pathogen interactions and biofilm formation. Our findings also confirmed the role of CPAR2_109520, CPAR2_200040, and CPAR2_500180 in pathogenesis. This study was the first attempt to use an overexpression strategy to systematically assess gene function in C. parapsilosis, and our results demonstrate that this approach is effective for such investigations. MDPI 2021-01-29 /pmc/articles/PMC7911391/ /pubmed/33572958 http://dx.doi.org/10.3390/jof7020097 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pál, Sára E. Tóth, Renáta Nosanchuk, Joshua D. Vágvölgyi, Csaba Németh, Tibor Gácser, Attila A Candida parapsilosis Overexpression Collection Reveals Genes Required for Pathogenesis |
title | A Candida parapsilosis Overexpression Collection Reveals Genes Required for Pathogenesis |
title_full | A Candida parapsilosis Overexpression Collection Reveals Genes Required for Pathogenesis |
title_fullStr | A Candida parapsilosis Overexpression Collection Reveals Genes Required for Pathogenesis |
title_full_unstemmed | A Candida parapsilosis Overexpression Collection Reveals Genes Required for Pathogenesis |
title_short | A Candida parapsilosis Overexpression Collection Reveals Genes Required for Pathogenesis |
title_sort | candida parapsilosis overexpression collection reveals genes required for pathogenesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911391/ https://www.ncbi.nlm.nih.gov/pubmed/33572958 http://dx.doi.org/10.3390/jof7020097 |
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