Cargando…
Indirect Somatic Embryogenesis and Cryopreservation of Agave tequilana Weber Cultivar ‘Chato’
Agave tequilana Weber cultivar ‘Chato’ represents an important genetic supply of wild severely in decline populations of ‘Chato’ for breeding and transformation programs. In this work, the indirect somatic embryogenesis and cryopreservation of Somatic Embryos (SEs) were investigated using the ‘Chato...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911407/ https://www.ncbi.nlm.nih.gov/pubmed/33525378 http://dx.doi.org/10.3390/plants10020249 |
_version_ | 1783656334448656384 |
---|---|
author | Delgado-Aceves, Lourdes González-Arnao, María Teresa Santacruz-Ruvalcaba, Fernando Folgado, Raquel Portillo, Liberato |
author_facet | Delgado-Aceves, Lourdes González-Arnao, María Teresa Santacruz-Ruvalcaba, Fernando Folgado, Raquel Portillo, Liberato |
author_sort | Delgado-Aceves, Lourdes |
collection | PubMed |
description | Agave tequilana Weber cultivar ‘Chato’ represents an important genetic supply of wild severely in decline populations of ‘Chato’ for breeding and transformation programs. In this work, the indirect somatic embryogenesis and cryopreservation of Somatic Embryos (SEs) were investigated using the ‘Chato’ cultivar as a study case. Methods: Embryogenic calli were induced by the cultivation of 1 cm of young leaves from in vitro plants on MS semisolid medium supplemented with 24.84, 33.13, 41.41, 49.69, and 57.98 μM 4-amino-3,5,6-trichloro-2- pyridinecarboxylic acid (picloram) in combination with 2.21, 3.32, and 4.43 μM 6-benzylaminopurine (BAP). The origin and structure of formed SEs were verified by histological analysis. Cryopreservation studies of SEs were performed following the V-cryoplate technique and using for dehydration two vitrification solutions (PVS2 and PVS3). Results: The highest average (52.43 ± 5.74) of produced SEs and the Embryo Forming Capacity (estimated index 52.43) were obtained using 49.69 µM picloram and 3.32 µM BAP in the culture medium. The highest post-cryopreservation regrowth (83%) and plant conversion rate (around 70%) were achieved with PVS2 at 0 °C for 15 min. Conclusion: Our work provides new advances about somatic embryogenesis in Agave and reports the first results on cryopreservation of SEs of this species. |
format | Online Article Text |
id | pubmed-7911407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79114072021-02-28 Indirect Somatic Embryogenesis and Cryopreservation of Agave tequilana Weber Cultivar ‘Chato’ Delgado-Aceves, Lourdes González-Arnao, María Teresa Santacruz-Ruvalcaba, Fernando Folgado, Raquel Portillo, Liberato Plants (Basel) Article Agave tequilana Weber cultivar ‘Chato’ represents an important genetic supply of wild severely in decline populations of ‘Chato’ for breeding and transformation programs. In this work, the indirect somatic embryogenesis and cryopreservation of Somatic Embryos (SEs) were investigated using the ‘Chato’ cultivar as a study case. Methods: Embryogenic calli were induced by the cultivation of 1 cm of young leaves from in vitro plants on MS semisolid medium supplemented with 24.84, 33.13, 41.41, 49.69, and 57.98 μM 4-amino-3,5,6-trichloro-2- pyridinecarboxylic acid (picloram) in combination with 2.21, 3.32, and 4.43 μM 6-benzylaminopurine (BAP). The origin and structure of formed SEs were verified by histological analysis. Cryopreservation studies of SEs were performed following the V-cryoplate technique and using for dehydration two vitrification solutions (PVS2 and PVS3). Results: The highest average (52.43 ± 5.74) of produced SEs and the Embryo Forming Capacity (estimated index 52.43) were obtained using 49.69 µM picloram and 3.32 µM BAP in the culture medium. The highest post-cryopreservation regrowth (83%) and plant conversion rate (around 70%) were achieved with PVS2 at 0 °C for 15 min. Conclusion: Our work provides new advances about somatic embryogenesis in Agave and reports the first results on cryopreservation of SEs of this species. MDPI 2021-01-28 /pmc/articles/PMC7911407/ /pubmed/33525378 http://dx.doi.org/10.3390/plants10020249 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Delgado-Aceves, Lourdes González-Arnao, María Teresa Santacruz-Ruvalcaba, Fernando Folgado, Raquel Portillo, Liberato Indirect Somatic Embryogenesis and Cryopreservation of Agave tequilana Weber Cultivar ‘Chato’ |
title | Indirect Somatic Embryogenesis and Cryopreservation of Agave tequilana Weber Cultivar ‘Chato’ |
title_full | Indirect Somatic Embryogenesis and Cryopreservation of Agave tequilana Weber Cultivar ‘Chato’ |
title_fullStr | Indirect Somatic Embryogenesis and Cryopreservation of Agave tequilana Weber Cultivar ‘Chato’ |
title_full_unstemmed | Indirect Somatic Embryogenesis and Cryopreservation of Agave tequilana Weber Cultivar ‘Chato’ |
title_short | Indirect Somatic Embryogenesis and Cryopreservation of Agave tequilana Weber Cultivar ‘Chato’ |
title_sort | indirect somatic embryogenesis and cryopreservation of agave tequilana weber cultivar ‘chato’ |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911407/ https://www.ncbi.nlm.nih.gov/pubmed/33525378 http://dx.doi.org/10.3390/plants10020249 |
work_keys_str_mv | AT delgadoaceveslourdes indirectsomaticembryogenesisandcryopreservationofagavetequilanawebercultivarchato AT gonzalezarnaomariateresa indirectsomaticembryogenesisandcryopreservationofagavetequilanawebercultivarchato AT santacruzruvalcabafernando indirectsomaticembryogenesisandcryopreservationofagavetequilanawebercultivarchato AT folgadoraquel indirectsomaticembryogenesisandcryopreservationofagavetequilanawebercultivarchato AT portilloliberato indirectsomaticembryogenesisandcryopreservationofagavetequilanawebercultivarchato |