Cargando…

Indirect Somatic Embryogenesis and Cryopreservation of Agave tequilana Weber Cultivar ‘Chato’

Agave tequilana Weber cultivar ‘Chato’ represents an important genetic supply of wild severely in decline populations of ‘Chato’ for breeding and transformation programs. In this work, the indirect somatic embryogenesis and cryopreservation of Somatic Embryos (SEs) were investigated using the ‘Chato...

Descripción completa

Detalles Bibliográficos
Autores principales: Delgado-Aceves, Lourdes, González-Arnao, María Teresa, Santacruz-Ruvalcaba, Fernando, Folgado, Raquel, Portillo, Liberato
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911407/
https://www.ncbi.nlm.nih.gov/pubmed/33525378
http://dx.doi.org/10.3390/plants10020249
_version_ 1783656334448656384
author Delgado-Aceves, Lourdes
González-Arnao, María Teresa
Santacruz-Ruvalcaba, Fernando
Folgado, Raquel
Portillo, Liberato
author_facet Delgado-Aceves, Lourdes
González-Arnao, María Teresa
Santacruz-Ruvalcaba, Fernando
Folgado, Raquel
Portillo, Liberato
author_sort Delgado-Aceves, Lourdes
collection PubMed
description Agave tequilana Weber cultivar ‘Chato’ represents an important genetic supply of wild severely in decline populations of ‘Chato’ for breeding and transformation programs. In this work, the indirect somatic embryogenesis and cryopreservation of Somatic Embryos (SEs) were investigated using the ‘Chato’ cultivar as a study case. Methods: Embryogenic calli were induced by the cultivation of 1 cm of young leaves from in vitro plants on MS semisolid medium supplemented with 24.84, 33.13, 41.41, 49.69, and 57.98 μM 4-amino-3,5,6-trichloro-2- pyridinecarboxylic acid (picloram) in combination with 2.21, 3.32, and 4.43 μM 6-benzylaminopurine (BAP). The origin and structure of formed SEs were verified by histological analysis. Cryopreservation studies of SEs were performed following the V-cryoplate technique and using for dehydration two vitrification solutions (PVS2 and PVS3). Results: The highest average (52.43 ± 5.74) of produced SEs and the Embryo Forming Capacity (estimated index 52.43) were obtained using 49.69 µM picloram and 3.32 µM BAP in the culture medium. The highest post-cryopreservation regrowth (83%) and plant conversion rate (around 70%) were achieved with PVS2 at 0 °C for 15 min. Conclusion: Our work provides new advances about somatic embryogenesis in Agave and reports the first results on cryopreservation of SEs of this species.
format Online
Article
Text
id pubmed-7911407
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-79114072021-02-28 Indirect Somatic Embryogenesis and Cryopreservation of Agave tequilana Weber Cultivar ‘Chato’ Delgado-Aceves, Lourdes González-Arnao, María Teresa Santacruz-Ruvalcaba, Fernando Folgado, Raquel Portillo, Liberato Plants (Basel) Article Agave tequilana Weber cultivar ‘Chato’ represents an important genetic supply of wild severely in decline populations of ‘Chato’ for breeding and transformation programs. In this work, the indirect somatic embryogenesis and cryopreservation of Somatic Embryos (SEs) were investigated using the ‘Chato’ cultivar as a study case. Methods: Embryogenic calli were induced by the cultivation of 1 cm of young leaves from in vitro plants on MS semisolid medium supplemented with 24.84, 33.13, 41.41, 49.69, and 57.98 μM 4-amino-3,5,6-trichloro-2- pyridinecarboxylic acid (picloram) in combination with 2.21, 3.32, and 4.43 μM 6-benzylaminopurine (BAP). The origin and structure of formed SEs were verified by histological analysis. Cryopreservation studies of SEs were performed following the V-cryoplate technique and using for dehydration two vitrification solutions (PVS2 and PVS3). Results: The highest average (52.43 ± 5.74) of produced SEs and the Embryo Forming Capacity (estimated index 52.43) were obtained using 49.69 µM picloram and 3.32 µM BAP in the culture medium. The highest post-cryopreservation regrowth (83%) and plant conversion rate (around 70%) were achieved with PVS2 at 0 °C for 15 min. Conclusion: Our work provides new advances about somatic embryogenesis in Agave and reports the first results on cryopreservation of SEs of this species. MDPI 2021-01-28 /pmc/articles/PMC7911407/ /pubmed/33525378 http://dx.doi.org/10.3390/plants10020249 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Delgado-Aceves, Lourdes
González-Arnao, María Teresa
Santacruz-Ruvalcaba, Fernando
Folgado, Raquel
Portillo, Liberato
Indirect Somatic Embryogenesis and Cryopreservation of Agave tequilana Weber Cultivar ‘Chato’
title Indirect Somatic Embryogenesis and Cryopreservation of Agave tequilana Weber Cultivar ‘Chato’
title_full Indirect Somatic Embryogenesis and Cryopreservation of Agave tequilana Weber Cultivar ‘Chato’
title_fullStr Indirect Somatic Embryogenesis and Cryopreservation of Agave tequilana Weber Cultivar ‘Chato’
title_full_unstemmed Indirect Somatic Embryogenesis and Cryopreservation of Agave tequilana Weber Cultivar ‘Chato’
title_short Indirect Somatic Embryogenesis and Cryopreservation of Agave tequilana Weber Cultivar ‘Chato’
title_sort indirect somatic embryogenesis and cryopreservation of agave tequilana weber cultivar ‘chato’
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911407/
https://www.ncbi.nlm.nih.gov/pubmed/33525378
http://dx.doi.org/10.3390/plants10020249
work_keys_str_mv AT delgadoaceveslourdes indirectsomaticembryogenesisandcryopreservationofagavetequilanawebercultivarchato
AT gonzalezarnaomariateresa indirectsomaticembryogenesisandcryopreservationofagavetequilanawebercultivarchato
AT santacruzruvalcabafernando indirectsomaticembryogenesisandcryopreservationofagavetequilanawebercultivarchato
AT folgadoraquel indirectsomaticembryogenesisandcryopreservationofagavetequilanawebercultivarchato
AT portilloliberato indirectsomaticembryogenesisandcryopreservationofagavetequilanawebercultivarchato