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Interactions of Bacillus subtilis Basement Spore Coat Layer Proteins

Bacillus subtilis endospores are exceptionally resistant cells encircled by two protective layers: a petidoglycan layer, termed the cortex, and the spore coat, a proteinaceous layer. The formation of both structures depends upon the proper assembly of a basement coat layer, which is composed of two...

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Autores principales: Krajčíková, Daniela, Bugárová, Veronika, Barák, Imrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911427/
https://www.ncbi.nlm.nih.gov/pubmed/33573199
http://dx.doi.org/10.3390/microorganisms9020285
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author Krajčíková, Daniela
Bugárová, Veronika
Barák, Imrich
author_facet Krajčíková, Daniela
Bugárová, Veronika
Barák, Imrich
author_sort Krajčíková, Daniela
collection PubMed
description Bacillus subtilis endospores are exceptionally resistant cells encircled by two protective layers: a petidoglycan layer, termed the cortex, and the spore coat, a proteinaceous layer. The formation of both structures depends upon the proper assembly of a basement coat layer, which is composed of two proteins, SpoIVA and SpoVM. The present work examines the interactions of SpoIVA and SpoVM with coat proteins recruited to the spore surface during the early stages of coat assembly. We showed that the alanine racemase YncD associates with two morphogenetic proteins, SpoIVA and CotE. Mutant spores lacking the yncD gene were less resistant against wet heat and germinated to a greater extent than wild-type spores in the presence of micromolar concentrations of l-alanine. In seeking a link between the coat and cortex formation, we investigated the interactions between SpoVM and SpoIVA and the proteins essential for cortex synthesis and found that SpoVM interacts with a penicillin-binding protein, SpoVD, and we also demonstrated that SpoVM is crucial for the proper localization of SpoVD. This study shows that direct contacts between coat morphogenetic proteins with a complex of cortex-synthesizing proteins could be one of the tools by which bacteria couple cortex and coat formation.
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spelling pubmed-79114272021-02-28 Interactions of Bacillus subtilis Basement Spore Coat Layer Proteins Krajčíková, Daniela Bugárová, Veronika Barák, Imrich Microorganisms Article Bacillus subtilis endospores are exceptionally resistant cells encircled by two protective layers: a petidoglycan layer, termed the cortex, and the spore coat, a proteinaceous layer. The formation of both structures depends upon the proper assembly of a basement coat layer, which is composed of two proteins, SpoIVA and SpoVM. The present work examines the interactions of SpoIVA and SpoVM with coat proteins recruited to the spore surface during the early stages of coat assembly. We showed that the alanine racemase YncD associates with two morphogenetic proteins, SpoIVA and CotE. Mutant spores lacking the yncD gene were less resistant against wet heat and germinated to a greater extent than wild-type spores in the presence of micromolar concentrations of l-alanine. In seeking a link between the coat and cortex formation, we investigated the interactions between SpoVM and SpoIVA and the proteins essential for cortex synthesis and found that SpoVM interacts with a penicillin-binding protein, SpoVD, and we also demonstrated that SpoVM is crucial for the proper localization of SpoVD. This study shows that direct contacts between coat morphogenetic proteins with a complex of cortex-synthesizing proteins could be one of the tools by which bacteria couple cortex and coat formation. MDPI 2021-01-30 /pmc/articles/PMC7911427/ /pubmed/33573199 http://dx.doi.org/10.3390/microorganisms9020285 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Krajčíková, Daniela
Bugárová, Veronika
Barák, Imrich
Interactions of Bacillus subtilis Basement Spore Coat Layer Proteins
title Interactions of Bacillus subtilis Basement Spore Coat Layer Proteins
title_full Interactions of Bacillus subtilis Basement Spore Coat Layer Proteins
title_fullStr Interactions of Bacillus subtilis Basement Spore Coat Layer Proteins
title_full_unstemmed Interactions of Bacillus subtilis Basement Spore Coat Layer Proteins
title_short Interactions of Bacillus subtilis Basement Spore Coat Layer Proteins
title_sort interactions of bacillus subtilis basement spore coat layer proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911427/
https://www.ncbi.nlm.nih.gov/pubmed/33573199
http://dx.doi.org/10.3390/microorganisms9020285
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