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DNA Methylation Profiles of Tph1A and BDNF in Gut and Brain of L. Rhamnosus-Treated Zebrafish
The bidirectional microbiota–gut–brain axis has raised increasing interest over the past years in the context of health and disease, but there is a lack of information on molecular mechanisms underlying this connection. We hypothesized that change in microbiota composition may affect brain epigeneti...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911505/ https://www.ncbi.nlm.nih.gov/pubmed/33499115 http://dx.doi.org/10.3390/biom11020142 |
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author | Cuomo, Mariella Borrelli, Luca Della Monica, Rosa Coretti, Lorena De Riso, Giulia D’Angelo Lancellotti di Durazzo, Luna Fioretti, Alessandro Lembo, Francesca Dinan, Timothy G. Cryan, John F. Cocozza, Sergio Chiariotti, Lorenzo |
author_facet | Cuomo, Mariella Borrelli, Luca Della Monica, Rosa Coretti, Lorena De Riso, Giulia D’Angelo Lancellotti di Durazzo, Luna Fioretti, Alessandro Lembo, Francesca Dinan, Timothy G. Cryan, John F. Cocozza, Sergio Chiariotti, Lorenzo |
author_sort | Cuomo, Mariella |
collection | PubMed |
description | The bidirectional microbiota–gut–brain axis has raised increasing interest over the past years in the context of health and disease, but there is a lack of information on molecular mechanisms underlying this connection. We hypothesized that change in microbiota composition may affect brain epigenetics leading to long-lasting effects on specific brain gene regulation. To test this hypothesis, we used Zebrafish (Danio Rerio) as a model system. As previously shown, treatment with high doses of probiotics can modulate behavior in Zebrafish, causing significant changes in the expression of some brain-relevant genes, such as BDNF and Tph1A. Using an ultra-deep targeted analysis, we investigated the methylation state of the BDNF and Tph1A promoter region in the brain and gut of probiotic-treated and untreated Zebrafishes. Thanks to the high resolution power of our analysis, we evaluated cell-to-cell methylation differences. At this resolution level, we found slight DNA methylation changes in probiotic-treated samples, likely related to a subgroup of brain and gut cells, and that specific DNA methylation signatures significantly correlated with specific behavioral scores. |
format | Online Article Text |
id | pubmed-7911505 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79115052021-02-28 DNA Methylation Profiles of Tph1A and BDNF in Gut and Brain of L. Rhamnosus-Treated Zebrafish Cuomo, Mariella Borrelli, Luca Della Monica, Rosa Coretti, Lorena De Riso, Giulia D’Angelo Lancellotti di Durazzo, Luna Fioretti, Alessandro Lembo, Francesca Dinan, Timothy G. Cryan, John F. Cocozza, Sergio Chiariotti, Lorenzo Biomolecules Article The bidirectional microbiota–gut–brain axis has raised increasing interest over the past years in the context of health and disease, but there is a lack of information on molecular mechanisms underlying this connection. We hypothesized that change in microbiota composition may affect brain epigenetics leading to long-lasting effects on specific brain gene regulation. To test this hypothesis, we used Zebrafish (Danio Rerio) as a model system. As previously shown, treatment with high doses of probiotics can modulate behavior in Zebrafish, causing significant changes in the expression of some brain-relevant genes, such as BDNF and Tph1A. Using an ultra-deep targeted analysis, we investigated the methylation state of the BDNF and Tph1A promoter region in the brain and gut of probiotic-treated and untreated Zebrafishes. Thanks to the high resolution power of our analysis, we evaluated cell-to-cell methylation differences. At this resolution level, we found slight DNA methylation changes in probiotic-treated samples, likely related to a subgroup of brain and gut cells, and that specific DNA methylation signatures significantly correlated with specific behavioral scores. MDPI 2021-01-22 /pmc/articles/PMC7911505/ /pubmed/33499115 http://dx.doi.org/10.3390/biom11020142 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cuomo, Mariella Borrelli, Luca Della Monica, Rosa Coretti, Lorena De Riso, Giulia D’Angelo Lancellotti di Durazzo, Luna Fioretti, Alessandro Lembo, Francesca Dinan, Timothy G. Cryan, John F. Cocozza, Sergio Chiariotti, Lorenzo DNA Methylation Profiles of Tph1A and BDNF in Gut and Brain of L. Rhamnosus-Treated Zebrafish |
title | DNA Methylation Profiles of Tph1A and BDNF in Gut and Brain of L. Rhamnosus-Treated Zebrafish |
title_full | DNA Methylation Profiles of Tph1A and BDNF in Gut and Brain of L. Rhamnosus-Treated Zebrafish |
title_fullStr | DNA Methylation Profiles of Tph1A and BDNF in Gut and Brain of L. Rhamnosus-Treated Zebrafish |
title_full_unstemmed | DNA Methylation Profiles of Tph1A and BDNF in Gut and Brain of L. Rhamnosus-Treated Zebrafish |
title_short | DNA Methylation Profiles of Tph1A and BDNF in Gut and Brain of L. Rhamnosus-Treated Zebrafish |
title_sort | dna methylation profiles of tph1a and bdnf in gut and brain of l. rhamnosus-treated zebrafish |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911505/ https://www.ncbi.nlm.nih.gov/pubmed/33499115 http://dx.doi.org/10.3390/biom11020142 |
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