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DNA Methylation Profiles of Tph1A and BDNF in Gut and Brain of L. Rhamnosus-Treated Zebrafish

The bidirectional microbiota–gut–brain axis has raised increasing interest over the past years in the context of health and disease, but there is a lack of information on molecular mechanisms underlying this connection. We hypothesized that change in microbiota composition may affect brain epigeneti...

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Autores principales: Cuomo, Mariella, Borrelli, Luca, Della Monica, Rosa, Coretti, Lorena, De Riso, Giulia, D’Angelo Lancellotti di Durazzo, Luna, Fioretti, Alessandro, Lembo, Francesca, Dinan, Timothy G., Cryan, John F., Cocozza, Sergio, Chiariotti, Lorenzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911505/
https://www.ncbi.nlm.nih.gov/pubmed/33499115
http://dx.doi.org/10.3390/biom11020142
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author Cuomo, Mariella
Borrelli, Luca
Della Monica, Rosa
Coretti, Lorena
De Riso, Giulia
D’Angelo Lancellotti di Durazzo, Luna
Fioretti, Alessandro
Lembo, Francesca
Dinan, Timothy G.
Cryan, John F.
Cocozza, Sergio
Chiariotti, Lorenzo
author_facet Cuomo, Mariella
Borrelli, Luca
Della Monica, Rosa
Coretti, Lorena
De Riso, Giulia
D’Angelo Lancellotti di Durazzo, Luna
Fioretti, Alessandro
Lembo, Francesca
Dinan, Timothy G.
Cryan, John F.
Cocozza, Sergio
Chiariotti, Lorenzo
author_sort Cuomo, Mariella
collection PubMed
description The bidirectional microbiota–gut–brain axis has raised increasing interest over the past years in the context of health and disease, but there is a lack of information on molecular mechanisms underlying this connection. We hypothesized that change in microbiota composition may affect brain epigenetics leading to long-lasting effects on specific brain gene regulation. To test this hypothesis, we used Zebrafish (Danio Rerio) as a model system. As previously shown, treatment with high doses of probiotics can modulate behavior in Zebrafish, causing significant changes in the expression of some brain-relevant genes, such as BDNF and Tph1A. Using an ultra-deep targeted analysis, we investigated the methylation state of the BDNF and Tph1A promoter region in the brain and gut of probiotic-treated and untreated Zebrafishes. Thanks to the high resolution power of our analysis, we evaluated cell-to-cell methylation differences. At this resolution level, we found slight DNA methylation changes in probiotic-treated samples, likely related to a subgroup of brain and gut cells, and that specific DNA methylation signatures significantly correlated with specific behavioral scores.
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spelling pubmed-79115052021-02-28 DNA Methylation Profiles of Tph1A and BDNF in Gut and Brain of L. Rhamnosus-Treated Zebrafish Cuomo, Mariella Borrelli, Luca Della Monica, Rosa Coretti, Lorena De Riso, Giulia D’Angelo Lancellotti di Durazzo, Luna Fioretti, Alessandro Lembo, Francesca Dinan, Timothy G. Cryan, John F. Cocozza, Sergio Chiariotti, Lorenzo Biomolecules Article The bidirectional microbiota–gut–brain axis has raised increasing interest over the past years in the context of health and disease, but there is a lack of information on molecular mechanisms underlying this connection. We hypothesized that change in microbiota composition may affect brain epigenetics leading to long-lasting effects on specific brain gene regulation. To test this hypothesis, we used Zebrafish (Danio Rerio) as a model system. As previously shown, treatment with high doses of probiotics can modulate behavior in Zebrafish, causing significant changes in the expression of some brain-relevant genes, such as BDNF and Tph1A. Using an ultra-deep targeted analysis, we investigated the methylation state of the BDNF and Tph1A promoter region in the brain and gut of probiotic-treated and untreated Zebrafishes. Thanks to the high resolution power of our analysis, we evaluated cell-to-cell methylation differences. At this resolution level, we found slight DNA methylation changes in probiotic-treated samples, likely related to a subgroup of brain and gut cells, and that specific DNA methylation signatures significantly correlated with specific behavioral scores. MDPI 2021-01-22 /pmc/articles/PMC7911505/ /pubmed/33499115 http://dx.doi.org/10.3390/biom11020142 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cuomo, Mariella
Borrelli, Luca
Della Monica, Rosa
Coretti, Lorena
De Riso, Giulia
D’Angelo Lancellotti di Durazzo, Luna
Fioretti, Alessandro
Lembo, Francesca
Dinan, Timothy G.
Cryan, John F.
Cocozza, Sergio
Chiariotti, Lorenzo
DNA Methylation Profiles of Tph1A and BDNF in Gut and Brain of L. Rhamnosus-Treated Zebrafish
title DNA Methylation Profiles of Tph1A and BDNF in Gut and Brain of L. Rhamnosus-Treated Zebrafish
title_full DNA Methylation Profiles of Tph1A and BDNF in Gut and Brain of L. Rhamnosus-Treated Zebrafish
title_fullStr DNA Methylation Profiles of Tph1A and BDNF in Gut and Brain of L. Rhamnosus-Treated Zebrafish
title_full_unstemmed DNA Methylation Profiles of Tph1A and BDNF in Gut and Brain of L. Rhamnosus-Treated Zebrafish
title_short DNA Methylation Profiles of Tph1A and BDNF in Gut and Brain of L. Rhamnosus-Treated Zebrafish
title_sort dna methylation profiles of tph1a and bdnf in gut and brain of l. rhamnosus-treated zebrafish
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911505/
https://www.ncbi.nlm.nih.gov/pubmed/33499115
http://dx.doi.org/10.3390/biom11020142
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